Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases

RNA crosslinking and proximity ligation methods are used to identify transcriptome-wide base pairing interactions. Here, the authors report PARIS2 (psoralen analysis of RNA interactions and structures 2), a method for RNA duplex determination in vivo with higher efficiency than the previous PARIS me...

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Auteurs principaux: Minjie Zhang, Kongpan Li, Jianhui Bai, Willem A. Velema, Chengqing Yu, Ryan van Damme, Wilson H. Lee, Maia L. Corpuz, Jian-Fu Chen, Zhipeng Lu
Format: article
Langue:EN
Publié: Nature Portfolio 2021
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Accès en ligne:https://doaj.org/article/84b70984cb2f4a888c00803699c67bcd
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Résumé:RNA crosslinking and proximity ligation methods are used to identify transcriptome-wide base pairing interactions. Here, the authors report PARIS2 (psoralen analysis of RNA interactions and structures 2), a method for RNA duplex determination in vivo with higher efficiency than the previous PARIS method.