Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases

RNA crosslinking and proximity ligation methods are used to identify transcriptome-wide base pairing interactions. Here, the authors report PARIS2 (psoralen analysis of RNA interactions and structures 2), a method for RNA duplex determination in vivo with higher efficiency than the previous PARIS me...

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Autores principales: Minjie Zhang, Kongpan Li, Jianhui Bai, Willem A. Velema, Chengqing Yu, Ryan van Damme, Wilson H. Lee, Maia L. Corpuz, Jian-Fu Chen, Zhipeng Lu
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/84b70984cb2f4a888c00803699c67bcd
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spelling oai:doaj.org-article:84b70984cb2f4a888c00803699c67bcd2021-12-02T18:28:38ZOptimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases10.1038/s41467-021-22552-y2041-1723https://doaj.org/article/84b70984cb2f4a888c00803699c67bcd2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22552-yhttps://doaj.org/toc/2041-1723RNA crosslinking and proximity ligation methods are used to identify transcriptome-wide base pairing interactions. Here, the authors report PARIS2 (psoralen analysis of RNA interactions and structures 2), a method for RNA duplex determination in vivo with higher efficiency than the previous PARIS method.Minjie ZhangKongpan LiJianhui BaiWillem A. VelemaChengqing YuRyan van DammeWilson H. LeeMaia L. CorpuzJian-Fu ChenZhipeng LuNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Minjie Zhang
Kongpan Li
Jianhui Bai
Willem A. Velema
Chengqing Yu
Ryan van Damme
Wilson H. Lee
Maia L. Corpuz
Jian-Fu Chen
Zhipeng Lu
Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases
description RNA crosslinking and proximity ligation methods are used to identify transcriptome-wide base pairing interactions. Here, the authors report PARIS2 (psoralen analysis of RNA interactions and structures 2), a method for RNA duplex determination in vivo with higher efficiency than the previous PARIS method.
format article
author Minjie Zhang
Kongpan Li
Jianhui Bai
Willem A. Velema
Chengqing Yu
Ryan van Damme
Wilson H. Lee
Maia L. Corpuz
Jian-Fu Chen
Zhipeng Lu
author_facet Minjie Zhang
Kongpan Li
Jianhui Bai
Willem A. Velema
Chengqing Yu
Ryan van Damme
Wilson H. Lee
Maia L. Corpuz
Jian-Fu Chen
Zhipeng Lu
author_sort Minjie Zhang
title Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases
title_short Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases
title_full Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases
title_fullStr Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases
title_full_unstemmed Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases
title_sort optimized photochemistry enables efficient analysis of dynamic rna structuromes and interactomes in genetic and infectious diseases
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/84b70984cb2f4a888c00803699c67bcd
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AT jianhuibai optimizedphotochemistryenablesefficientanalysisofdynamicrnastructuromesandinteractomesingeneticandinfectiousdiseases
AT willemavelema optimizedphotochemistryenablesefficientanalysisofdynamicrnastructuromesandinteractomesingeneticandinfectiousdiseases
AT chengqingyu optimizedphotochemistryenablesefficientanalysisofdynamicrnastructuromesandinteractomesingeneticandinfectiousdiseases
AT ryanvandamme optimizedphotochemistryenablesefficientanalysisofdynamicrnastructuromesandinteractomesingeneticandinfectiousdiseases
AT wilsonhlee optimizedphotochemistryenablesefficientanalysisofdynamicrnastructuromesandinteractomesingeneticandinfectiousdiseases
AT maialcorpuz optimizedphotochemistryenablesefficientanalysisofdynamicrnastructuromesandinteractomesingeneticandinfectiousdiseases
AT jianfuchen optimizedphotochemistryenablesefficientanalysisofdynamicrnastructuromesandinteractomesingeneticandinfectiousdiseases
AT zhipenglu optimizedphotochemistryenablesefficientanalysisofdynamicrnastructuromesandinteractomesingeneticandinfectiousdiseases
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