Towards positional isolation of three quantitative trait loci conferring resistance to powdery mildew in two Spanish barley landraces.

Three quantitative trait loci (QTL) conferring broad spectrum resistance to powdery mildew, caused by the fungus Blumeria graminis f. sp. hordei, were previously identified on chromosomes 7HS, 7HL and 6HL in the Spanish barley landrace-derived lines SBCC097 and SBCC145. In the present work, a genome...

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Autores principales:	Cristina Silvar, Dragan Perovic, Thomas Nussbaumer, Manuel Spannagl, Björn Usadel, Ana Casas, Ernesto Igartua, Frank Ordon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/84c8067ee6da45dba2d1fcfca5f1a2fc
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spelling	oai:doaj.org-article:84c8067ee6da45dba2d1fcfca5f1a2fc2021-11-18T07:40:18ZTowards positional isolation of three quantitative trait loci conferring resistance to powdery mildew in two Spanish barley landraces.1932-620310.1371/journal.pone.0067336https://doaj.org/article/84c8067ee6da45dba2d1fcfca5f1a2fc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23826271/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Three quantitative trait loci (QTL) conferring broad spectrum resistance to powdery mildew, caused by the fungus Blumeria graminis f. sp. hordei, were previously identified on chromosomes 7HS, 7HL and 6HL in the Spanish barley landrace-derived lines SBCC097 and SBCC145. In the present work, a genome-wide putative linear gene index of barley (Genome Zipper) and the first draft of the physical, genetic and functional sequence of the barley genome were used to go one step further in the shortening and explicit demarcation on the barley genome of these regions conferring resistance to powdery mildew as well as in the identification of candidate genes. First, a comparative analysis of the target regions to the barley Genome Zippers of chromosomes 7H and 6H allowed the development of 25 new gene-based molecular markers, which slightly better delimit the QTL intervals. These new markers provided the framework for anchoring of genetic and physical maps, figuring out the outline of the barley genome at the target regions in SBCC097 and SBCC145. The outermost flanking markers of QTLs on 7HS, 7HL and 6HL defined a physical area of 4 Mb, 3.7 Mb and 3.2 Mb, respectively. In total, 21, 10 and 16 genes on 7HS, 7HL and 6HL, respectively, could be interpreted as potential candidates to explain the resistance to powdery mildew, as they encode proteins of related functions with respect to the known pathogen defense-related processes. The majority of these were annotated as belonging to the NBS-LRR class or protein kinase family.Cristina SilvarDragan PerovicThomas NussbaumerManuel SpannaglBjörn UsadelAna CasasErnesto IgartuaFrank OrdonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e67336 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristina Silvar Dragan Perovic Thomas Nussbaumer Manuel Spannagl Björn Usadel Ana Casas Ernesto Igartua Frank Ordon Towards positional isolation of three quantitative trait loci conferring resistance to powdery mildew in two Spanish barley landraces.
description	Three quantitative trait loci (QTL) conferring broad spectrum resistance to powdery mildew, caused by the fungus Blumeria graminis f. sp. hordei, were previously identified on chromosomes 7HS, 7HL and 6HL in the Spanish barley landrace-derived lines SBCC097 and SBCC145. In the present work, a genome-wide putative linear gene index of barley (Genome Zipper) and the first draft of the physical, genetic and functional sequence of the barley genome were used to go one step further in the shortening and explicit demarcation on the barley genome of these regions conferring resistance to powdery mildew as well as in the identification of candidate genes. First, a comparative analysis of the target regions to the barley Genome Zippers of chromosomes 7H and 6H allowed the development of 25 new gene-based molecular markers, which slightly better delimit the QTL intervals. These new markers provided the framework for anchoring of genetic and physical maps, figuring out the outline of the barley genome at the target regions in SBCC097 and SBCC145. The outermost flanking markers of QTLs on 7HS, 7HL and 6HL defined a physical area of 4 Mb, 3.7 Mb and 3.2 Mb, respectively. In total, 21, 10 and 16 genes on 7HS, 7HL and 6HL, respectively, could be interpreted as potential candidates to explain the resistance to powdery mildew, as they encode proteins of related functions with respect to the known pathogen defense-related processes. The majority of these were annotated as belonging to the NBS-LRR class or protein kinase family.
format	article
author	Cristina Silvar Dragan Perovic Thomas Nussbaumer Manuel Spannagl Björn Usadel Ana Casas Ernesto Igartua Frank Ordon
author_facet	Cristina Silvar Dragan Perovic Thomas Nussbaumer Manuel Spannagl Björn Usadel Ana Casas Ernesto Igartua Frank Ordon
author_sort	Cristina Silvar
title	Towards positional isolation of three quantitative trait loci conferring resistance to powdery mildew in two Spanish barley landraces.
title_short	Towards positional isolation of three quantitative trait loci conferring resistance to powdery mildew in two Spanish barley landraces.
title_full	Towards positional isolation of three quantitative trait loci conferring resistance to powdery mildew in two Spanish barley landraces.
title_fullStr	Towards positional isolation of three quantitative trait loci conferring resistance to powdery mildew in two Spanish barley landraces.
title_full_unstemmed	Towards positional isolation of three quantitative trait loci conferring resistance to powdery mildew in two Spanish barley landraces.
title_sort	towards positional isolation of three quantitative trait loci conferring resistance to powdery mildew in two spanish barley landraces.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/84c8067ee6da45dba2d1fcfca5f1a2fc
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Towards positional isolation of three quantitative trait loci conferring resistance to powdery mildew in two Spanish barley landraces.

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