<named-content content-type="genus-species">Neisseria gonorrhoeae</named-content> Metalloprotease NGO1686 Is Required for Full Piliation, and Piliation Is Required for Resistance to H<sub>2</sub>O<sub>2</sub>- and Neutrophil-Mediated Killing

ABSTRACT The sexually transmitted infection gonorrhea is caused exclusively by the human-specific pathogen Neisseria gonorrhoeae. Type IV pili are an essential virulence factor uniformly expressed on clinical gonococcal isolates and are required for several aspects of gonococcal pathogenesis, includ...

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Autores principales:	Elizabeth A. Stohl, Erin M. Dale, Alison K. Criss, H. Steven Seifert
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/84c8a14d254c4dea88bfd771be33f51e
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spelling	oai:doaj.org-article:84c8a14d254c4dea88bfd771be33f51e2021-11-15T15:43:08Z<named-content content-type="genus-species">Neisseria gonorrhoeae</named-content> Metalloprotease NGO1686 Is Required for Full Piliation, and Piliation Is Required for Resistance to H<sub>2</sub>O<sub>2</sub>- and Neutrophil-Mediated Killing10.1128/mBio.00399-132150-7511https://doaj.org/article/84c8a14d254c4dea88bfd771be33f51e2013-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00399-13https://doaj.org/toc/2150-7511ABSTRACT The sexually transmitted infection gonorrhea is caused exclusively by the human-specific pathogen Neisseria gonorrhoeae. Type IV pili are an essential virulence factor uniformly expressed on clinical gonococcal isolates and are required for several aspects of gonococcal pathogenesis, including adherence to host tissues, autoagglutination, twitching motility, and the uptake of DNA during transformation. Symptomatic gonococcal infection is characterized by the influx of neutrophils or polymorphonuclear leukocytes (PMNs) to the site of infection. PMNs are a key component of gonococcal pathogenesis, mediating the innate immune response through the use of oxidative and nonoxidative killing mechanisms. The M23B family zinc metallopeptidase NGO1686 is required for gonococci to survive oxidative killing by H2O2- and PMN-mediated killing through unknown mechanisms, but the only known target of NGO1686 is peptidoglycan. We report that the effect of NGO1686 on survival after exposure to H2O2 and PMNs is mediated through its role in elaborating pili and that nonpiliated mutants of N. gonorrhoeae are less resistant to killing by H2O2, LL-37, and PMNs than the corresponding piliated strains. These findings add to the various virulence-associated functions attributable to gonococcal pili and may explain the selection basis for piliation in clinical isolates of N. gonorrhoeae. IMPORTANCE Successful infectious agents need to overcome host defense systems to establish infection. We show that the Neisseria pilus, a major virulence factor of this organism, which causes gonorrhea, helps protect the bacterium from two major killing mechanisms used by the host to combat infections. We also show that to express the pilus, an enzyme needs to partially degrade the cell wall of the bacterium.Elizabeth A. StohlErin M. DaleAlison K. CrissH. Steven SeifertAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 4 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Elizabeth A. Stohl Erin M. Dale Alison K. Criss H. Steven Seifert <named-content content-type="genus-species">Neisseria gonorrhoeae</named-content> Metalloprotease NGO1686 Is Required for Full Piliation, and Piliation Is Required for Resistance to H<sub>2</sub>O<sub>2</sub>- and Neutrophil-Mediated Killing
description	ABSTRACT The sexually transmitted infection gonorrhea is caused exclusively by the human-specific pathogen Neisseria gonorrhoeae. Type IV pili are an essential virulence factor uniformly expressed on clinical gonococcal isolates and are required for several aspects of gonococcal pathogenesis, including adherence to host tissues, autoagglutination, twitching motility, and the uptake of DNA during transformation. Symptomatic gonococcal infection is characterized by the influx of neutrophils or polymorphonuclear leukocytes (PMNs) to the site of infection. PMNs are a key component of gonococcal pathogenesis, mediating the innate immune response through the use of oxidative and nonoxidative killing mechanisms. The M23B family zinc metallopeptidase NGO1686 is required for gonococci to survive oxidative killing by H2O2- and PMN-mediated killing through unknown mechanisms, but the only known target of NGO1686 is peptidoglycan. We report that the effect of NGO1686 on survival after exposure to H2O2 and PMNs is mediated through its role in elaborating pili and that nonpiliated mutants of N. gonorrhoeae are less resistant to killing by H2O2, LL-37, and PMNs than the corresponding piliated strains. These findings add to the various virulence-associated functions attributable to gonococcal pili and may explain the selection basis for piliation in clinical isolates of N. gonorrhoeae. IMPORTANCE Successful infectious agents need to overcome host defense systems to establish infection. We show that the Neisseria pilus, a major virulence factor of this organism, which causes gonorrhea, helps protect the bacterium from two major killing mechanisms used by the host to combat infections. We also show that to express the pilus, an enzyme needs to partially degrade the cell wall of the bacterium.
format	article
author	Elizabeth A. Stohl Erin M. Dale Alison K. Criss H. Steven Seifert
author_facet	Elizabeth A. Stohl Erin M. Dale Alison K. Criss H. Steven Seifert
author_sort	Elizabeth A. Stohl
title	<named-content content-type="genus-species">Neisseria gonorrhoeae</named-content> Metalloprotease NGO1686 Is Required for Full Piliation, and Piliation Is Required for Resistance to H<sub>2</sub>O<sub>2</sub>- and Neutrophil-Mediated Killing
title_short	<named-content content-type="genus-species">Neisseria gonorrhoeae</named-content> Metalloprotease NGO1686 Is Required for Full Piliation, and Piliation Is Required for Resistance to H<sub>2</sub>O<sub>2</sub>- and Neutrophil-Mediated Killing
title_full	<named-content content-type="genus-species">Neisseria gonorrhoeae</named-content> Metalloprotease NGO1686 Is Required for Full Piliation, and Piliation Is Required for Resistance to H<sub>2</sub>O<sub>2</sub>- and Neutrophil-Mediated Killing
title_fullStr	<named-content content-type="genus-species">Neisseria gonorrhoeae</named-content> Metalloprotease NGO1686 Is Required for Full Piliation, and Piliation Is Required for Resistance to H<sub>2</sub>O<sub>2</sub>- and Neutrophil-Mediated Killing
title_full_unstemmed	<named-content content-type="genus-species">Neisseria gonorrhoeae</named-content> Metalloprotease NGO1686 Is Required for Full Piliation, and Piliation Is Required for Resistance to H<sub>2</sub>O<sub>2</sub>- and Neutrophil-Mediated Killing
title_sort	<named-content content-type="genus-species">neisseria gonorrhoeae</named-content> metalloprotease ngo1686 is required for full piliation, and piliation is required for resistance to h<sub>2</sub>o<sub>2</sub>- and neutrophil-mediated killing
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/84c8a14d254c4dea88bfd771be33f51e
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<named-content content-type="genus-species">Neisseria gonorrhoeae</named-content> Metalloprotease NGO1686 Is Required for Full Piliation, and Piliation Is Required for Resistance to H<sub>2</sub>O<sub>2</sub>- and Neutrophil-Mediated Killing

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