Inhibition of the activated sludge-associated enzyme phosphatase by transition metal oxyanions

Organic esters of phosphoric acid and other organophosphorous compounds are enzymatically hydrolyzed during wastewater treatment by microbial phosphoesterases, especially by phosphomonoesterase (phosphatase). For physiological reasons, the enzyme is inhibited by its main inorganic reaction product,...

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Autores principales:	Beatriz Böger, Marion Wacht, Michaela Leuck, Raquel de O. Vilhena, Maria Riese, Klaus Fischer
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	activated sludge alkaline phosphatase enzyme inhibition tungstate vanadate wastewater treatment Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/84cc28d5a9554d43969162367b7f5a88
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spelling	oai:doaj.org-article:84cc28d5a9554d43969162367b7f5a882021-11-06T11:07:26ZInhibition of the activated sludge-associated enzyme phosphatase by transition metal oxyanions0273-12231996-973210.2166/wst.2021.172https://doaj.org/article/84cc28d5a9554d43969162367b7f5a882021-06-01T00:00:00Zhttp://wst.iwaponline.com/content/83/11/2629https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732Organic esters of phosphoric acid and other organophosphorous compounds are enzymatically hydrolyzed during wastewater treatment by microbial phosphoesterases, especially by phosphomonoesterase (phosphatase). For physiological reasons, the enzyme is inhibited by its main inorganic reaction product, ortho-phosphate. It is known that oxyanions of transition metals, resembling the molecular topology of ortho-phosphate, e.g. vanadate and tungstate, are more potent inhibitors for microbial alkaline phosphatase than phosphate. To proof this effect for activated sludge, a multitude of samples from a communal wastewater treatment plant was exposed at pH values from 7.00 to 8.50 to tungstate, vanadate, and molybdate. Inhibition effects were determined by a sensitive fluorimetric microplate assay and characteristic parameters (IC50 and IC20 concentrations) were deduced from modelled dose-response functions. Mean inhibitor concentrations (in brackets: ranges) causing 50% inactivation (IC50) at pH 7.50 were 2.5 (1.3–4.1) μM tungstate, 2.9 (1.6–5.5) μM vanadate, and 41.4 (33.6–56.7) μM molybdate. Vanadate and tungstate concentrations between 0.6 and 0.7 μM provoked a 20% (IC20) inhibition. The inhibition efficiency of tungstate and molybdate decreased with increasing pH, whereas vanadate reacted pH independently. These results underline the necessity to consider enzyme inhibition assessing the limitations and potentials of biological wastewater treatment processes. HIGHLIGHTS Sensitive fluorimetric microplate assay applied to analyze phosphatase inhibition.; Vanadate and tungstate are strong inhibitors of sludge-associated alkaline phosphatase.; Molybdate has lower inhibition potency than the other oxyanions.; Inhibition efficiency of tungstate and molybdate decreased with increasing pH.; Enzyme inhibition needs more attention in the performance assessment of wastewater treatment.;Beatriz BögerMarion WachtMichaela LeuckRaquel de O. VilhenaMaria RieseKlaus FischerIWA Publishingarticleactivated sludgealkaline phosphataseenzyme inhibitiontungstatevanadatewastewater treatmentEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 83, Iss 11, Pp 2629-2639 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	activated sludge alkaline phosphatase enzyme inhibition tungstate vanadate wastewater treatment Environmental technology. Sanitary engineering TD1-1066
spellingShingle	activated sludge alkaline phosphatase enzyme inhibition tungstate vanadate wastewater treatment Environmental technology. Sanitary engineering TD1-1066 Beatriz Böger Marion Wacht Michaela Leuck Raquel de O. Vilhena Maria Riese Klaus Fischer Inhibition of the activated sludge-associated enzyme phosphatase by transition metal oxyanions
description	Organic esters of phosphoric acid and other organophosphorous compounds are enzymatically hydrolyzed during wastewater treatment by microbial phosphoesterases, especially by phosphomonoesterase (phosphatase). For physiological reasons, the enzyme is inhibited by its main inorganic reaction product, ortho-phosphate. It is known that oxyanions of transition metals, resembling the molecular topology of ortho-phosphate, e.g. vanadate and tungstate, are more potent inhibitors for microbial alkaline phosphatase than phosphate. To proof this effect for activated sludge, a multitude of samples from a communal wastewater treatment plant was exposed at pH values from 7.00 to 8.50 to tungstate, vanadate, and molybdate. Inhibition effects were determined by a sensitive fluorimetric microplate assay and characteristic parameters (IC50 and IC20 concentrations) were deduced from modelled dose-response functions. Mean inhibitor concentrations (in brackets: ranges) causing 50% inactivation (IC50) at pH 7.50 were 2.5 (1.3–4.1) μM tungstate, 2.9 (1.6–5.5) μM vanadate, and 41.4 (33.6–56.7) μM molybdate. Vanadate and tungstate concentrations between 0.6 and 0.7 μM provoked a 20% (IC20) inhibition. The inhibition efficiency of tungstate and molybdate decreased with increasing pH, whereas vanadate reacted pH independently. These results underline the necessity to consider enzyme inhibition assessing the limitations and potentials of biological wastewater treatment processes. HIGHLIGHTS Sensitive fluorimetric microplate assay applied to analyze phosphatase inhibition.; Vanadate and tungstate are strong inhibitors of sludge-associated alkaline phosphatase.; Molybdate has lower inhibition potency than the other oxyanions.; Inhibition efficiency of tungstate and molybdate decreased with increasing pH.; Enzyme inhibition needs more attention in the performance assessment of wastewater treatment.;
format	article
author	Beatriz Böger Marion Wacht Michaela Leuck Raquel de O. Vilhena Maria Riese Klaus Fischer
author_facet	Beatriz Böger Marion Wacht Michaela Leuck Raquel de O. Vilhena Maria Riese Klaus Fischer
author_sort	Beatriz Böger
title	Inhibition of the activated sludge-associated enzyme phosphatase by transition metal oxyanions
title_short	Inhibition of the activated sludge-associated enzyme phosphatase by transition metal oxyanions
title_full	Inhibition of the activated sludge-associated enzyme phosphatase by transition metal oxyanions
title_fullStr	Inhibition of the activated sludge-associated enzyme phosphatase by transition metal oxyanions
title_full_unstemmed	Inhibition of the activated sludge-associated enzyme phosphatase by transition metal oxyanions
title_sort	inhibition of the activated sludge-associated enzyme phosphatase by transition metal oxyanions
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/84cc28d5a9554d43969162367b7f5a88
work_keys_str_mv	AT beatrizboger inhibitionoftheactivatedsludgeassociatedenzymephosphatasebytransitionmetaloxyanions AT marionwacht inhibitionoftheactivatedsludgeassociatedenzymephosphatasebytransitionmetaloxyanions AT michaelaleuck inhibitionoftheactivatedsludgeassociatedenzymephosphatasebytransitionmetaloxyanions AT raqueldeovilhena inhibitionoftheactivatedsludgeassociatedenzymephosphatasebytransitionmetaloxyanions AT mariariese inhibitionoftheactivatedsludgeassociatedenzymephosphatasebytransitionmetaloxyanions AT klausfischer inhibitionoftheactivatedsludgeassociatedenzymephosphatasebytransitionmetaloxyanions
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Inhibition of the activated sludge-associated enzyme phosphatase by transition metal oxyanions

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