Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells
Alokita Karmakar2, Stacie M Bratton1, Enkeleda Dervishi2, Anindya Ghosh3, Meena Mahmood2, Yang Xu2, Lamya Mohammed Saeed2, Thikra Mustafa2, Dan Casciano2, Anna Radominska-Pandya1, Alexandru S Biris21Biochemistry Department, University of Arkansas for Medical Sciences; 2Nanotechnology Center, Applied...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2011
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/84d647854c8d47919687049201c96301 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:84d647854c8d47919687049201c96301 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:84d647854c8d47919687049201c963012021-12-02T02:01:53ZEthylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells1176-91141178-2013https://doaj.org/article/84d647854c8d47919687049201c963012011-05-01T00:00:00Zhttp://www.dovepress.com/ethylenediamine-functionalized-single-walled-nanotube-f-swnt-assisted--a7486https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Alokita Karmakar2, Stacie M Bratton1, Enkeleda Dervishi2, Anindya Ghosh3, Meena Mahmood2, Yang Xu2, Lamya Mohammed Saeed2, Thikra Mustafa2, Dan Casciano2, Anna Radominska-Pandya1, Alexandru S Biris21Biochemistry Department, University of Arkansas for Medical Sciences; 2Nanotechnology Center, Applied Science Department; 3Department of Chemistry, University of Arkansas, Little Rock, AR, USAAbstract: A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 µg mL-1 and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials.Keywords: carbon nanotubes, gene delivery, cancer cells, p53 oncogene suppressorKarmakar ABratton SMDervishi EGhosh AMahmood MXu YSaeed LMustafa TCasciano DRadominska-Pandya ABiris ASDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1045-1055 (2011) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine (General) R5-920 |
| spellingShingle |
Medicine (General) R5-920 Karmakar A Bratton SM Dervishi E Ghosh A Mahmood M Xu Y Saeed L Mustafa T Casciano D Radominska-Pandya A Biris AS Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| description |
Alokita Karmakar2, Stacie M Bratton1, Enkeleda Dervishi2, Anindya Ghosh3, Meena Mahmood2, Yang Xu2, Lamya Mohammed Saeed2, Thikra Mustafa2, Dan Casciano2, Anna Radominska-Pandya1, Alexandru S Biris21Biochemistry Department, University of Arkansas for Medical Sciences; 2Nanotechnology Center, Applied Science Department; 3Department of Chemistry, University of Arkansas, Little Rock, AR, USAAbstract: A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 µg mL-1 and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials.Keywords: carbon nanotubes, gene delivery, cancer cells, p53 oncogene suppressor |
| format |
article |
| author |
Karmakar A Bratton SM Dervishi E Ghosh A Mahmood M Xu Y Saeed L Mustafa T Casciano D Radominska-Pandya A Biris AS |
| author_facet |
Karmakar A Bratton SM Dervishi E Ghosh A Mahmood M Xu Y Saeed L Mustafa T Casciano D Radominska-Pandya A Biris AS |
| author_sort |
Karmakar A |
| title |
Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| title_short |
Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| title_full |
Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| title_fullStr |
Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| title_full_unstemmed |
Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells |
| title_sort |
ethylenediamine functionalized-single-walled nanotube (f-swnt)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer mcf-7 cells |
| publisher |
Dove Medical Press |
| publishDate |
2011 |
| url |
https://doaj.org/article/84d647854c8d47919687049201c96301 |
| work_keys_str_mv |
AT karmakara ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells AT brattonsm ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells AT dervishie ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells AT ghosha ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells AT mahmoodm ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells AT xuy ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells AT saeedl ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells AT mustafat ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells AT cascianod ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells AT radominskapandyaa ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells AT birisas ethylenediaminefunctionalizedsinglewallednanotubefswntassistedinvitrodeliveryoftheoncogenesuppressorp53genetobreastcancermcf7cells |
| _version_ |
1718402788418912256 |