Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients

Abstract Background Escherichia coli is among the most common uropathogens. Increased antibiotic resistance in Gram negative bacilli is global concern. Alternative therapeutic options including vaccines against uropathogenic E. coli (UPEC) have been developed. In this study, we compared the genotypi...

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Autores principales:	Miri Hyun, Ji Yeon Lee, Hyun ah Kim
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Urinary tract infection Escherichia coli Phylogenetic group Virulence factor Antimicrobial susceptibility Therapeutics. Pharmacology RM1-950 Infectious and parasitic diseases RC109-216 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/84d8fd6e8ca54bdfbd6a6b3546e2cb50
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spelling	oai:doaj.org-article:84d8fd6e8ca54bdfbd6a6b3546e2cb502021-11-14T12:11:37ZDifferences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients10.1186/s12941-021-00481-41476-0711https://doaj.org/article/84d8fd6e8ca54bdfbd6a6b3546e2cb502021-11-01T00:00:00Zhttps://doi.org/10.1186/s12941-021-00481-4https://doaj.org/toc/1476-0711Abstract Background Escherichia coli is among the most common uropathogens. Increased antibiotic resistance in Gram negative bacilli is global concern. Alternative therapeutic options including vaccines against uropathogenic E. coli (UPEC) have been developed. In this study, we compared the genotypic characteristics and antimicrobial susceptibility of UPEC according to phylogenetic groups. Methods We retrospectively reviewed the medical records of pyelonephritis patients with UPEC between February 2015 and June 2018. The study was conducted at a medical center in Korea. We compared the clinical and genotypic characteristics of UPEC according to phylogenetic groups. The phylogenetic groups and 29 virulence factors were identified using multiplex polymerase chain reaction. Results Phylogenetic group analysis revealed that most uropathogenic E. coli belonged to groups B2 and D: B2 (276, 77.7%), D (62, 17.5%), B1 (12, 3.4%), and A (5, 1.4%). Among the virulence factors, fyuA, fimH, traT, iutA, papG allele II, and papC were the most frequently observed. Phylogenetic group B2 was more closely related to virulence factors, including fimH, sfa/focED, focG, hlyA, cnf1, fyuA, and PAI, than group D. Groups B2 and D showed similar clinical presentations and complications. Group B2 had mostly healthcare-associated infections and antimicrobial resistance. Group D mostly had community-acquired infections. The K1 serotype was prevalent in group B2, and K5 was the most prevalent in group D. Conclusions Phylogenetic group B2 had more proportions and types of virulence factors than group D. Group B2 showed a high presentation of virulence factors related to adhesions and toxins. An increased presentation of antimicrobial resistance and healthcare-associated infections was also noted. Considering the genetic characteristics of UPEC, alternative therapeutic options targeting frequent virulence factors might be considered in addition to antibiotics.Miri HyunJi Yeon LeeHyun ah KimBMCarticleUrinary tract infectionEscherichia coliPhylogenetic groupVirulence factorAntimicrobial susceptibilityTherapeutics. PharmacologyRM1-950Infectious and parasitic diseasesRC109-216MicrobiologyQR1-502ENAnnals of Clinical Microbiology and Antimicrobials, Vol 20, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Urinary tract infection Escherichia coli Phylogenetic group Virulence factor Antimicrobial susceptibility Therapeutics. Pharmacology RM1-950 Infectious and parasitic diseases RC109-216 Microbiology QR1-502
spellingShingle	Urinary tract infection Escherichia coli Phylogenetic group Virulence factor Antimicrobial susceptibility Therapeutics. Pharmacology RM1-950 Infectious and parasitic diseases RC109-216 Microbiology QR1-502 Miri Hyun Ji Yeon Lee Hyun ah Kim Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients
description	Abstract Background Escherichia coli is among the most common uropathogens. Increased antibiotic resistance in Gram negative bacilli is global concern. Alternative therapeutic options including vaccines against uropathogenic E. coli (UPEC) have been developed. In this study, we compared the genotypic characteristics and antimicrobial susceptibility of UPEC according to phylogenetic groups. Methods We retrospectively reviewed the medical records of pyelonephritis patients with UPEC between February 2015 and June 2018. The study was conducted at a medical center in Korea. We compared the clinical and genotypic characteristics of UPEC according to phylogenetic groups. The phylogenetic groups and 29 virulence factors were identified using multiplex polymerase chain reaction. Results Phylogenetic group analysis revealed that most uropathogenic E. coli belonged to groups B2 and D: B2 (276, 77.7%), D (62, 17.5%), B1 (12, 3.4%), and A (5, 1.4%). Among the virulence factors, fyuA, fimH, traT, iutA, papG allele II, and papC were the most frequently observed. Phylogenetic group B2 was more closely related to virulence factors, including fimH, sfa/focED, focG, hlyA, cnf1, fyuA, and PAI, than group D. Groups B2 and D showed similar clinical presentations and complications. Group B2 had mostly healthcare-associated infections and antimicrobial resistance. Group D mostly had community-acquired infections. The K1 serotype was prevalent in group B2, and K5 was the most prevalent in group D. Conclusions Phylogenetic group B2 had more proportions and types of virulence factors than group D. Group B2 showed a high presentation of virulence factors related to adhesions and toxins. An increased presentation of antimicrobial resistance and healthcare-associated infections was also noted. Considering the genetic characteristics of UPEC, alternative therapeutic options targeting frequent virulence factors might be considered in addition to antibiotics.
format	article
author	Miri Hyun Ji Yeon Lee Hyun ah Kim
author_facet	Miri Hyun Ji Yeon Lee Hyun ah Kim
author_sort	Miri Hyun
title	Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients
title_short	Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients
title_full	Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients
title_fullStr	Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients
title_full_unstemmed	Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients
title_sort	differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic escherichia coli strains isolated from korean patients
publisher	BMC
publishDate	2021
url	https://doaj.org/article/84d8fd6e8ca54bdfbd6a6b3546e2cb50
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_version_	1718429415636992000

Differences of virulence factors, and antimicrobial susceptibility according to phylogenetic group in uropathogenic Escherichia coli strains isolated from Korean patients

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