Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice

Abstract There is no simple method to measure glomerular filtration rate (GFR) in mice, which limits the use of mice in models of renal diseases. We aimed at simplifying the plasma clearance of iohexol in mice, using dried blood spot (DBS) sampling in order to reduce the amount of blood taken for an...

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Autores principales:	Ana Elena Rodríguez-Rodríguez, Sergio Luis-Lima, Javier Donate-Correa, Laura Diaz-Martín, María Rosa Arnau, Alejandro Jiménez-Sosa, Flavio Gaspari, Alberto Ortiz, Esteban Porrini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/84dec4fe7eb64ad9b3eb968af3079f7c
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spelling	oai:doaj.org-article:84dec4fe7eb64ad9b3eb968af3079f7c2021-12-02T11:35:53ZIohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice10.1038/s41598-021-83934-22045-2322https://doaj.org/article/84dec4fe7eb64ad9b3eb968af3079f7c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83934-2https://doaj.org/toc/2045-2322Abstract There is no simple method to measure glomerular filtration rate (GFR) in mice, which limits the use of mice in models of renal diseases. We aimed at simplifying the plasma clearance of iohexol in mice, using dried blood spot (DBS) sampling in order to reduce the amount of blood taken for analysis. GFR was measured simultaneously by a reference method in total blood—as described before—and tested method using DBS in fifteen male and six female C57BL/6J mice. Total blood extraction was 50 μL for the reference methods and 25μL for the tested methods, distributed in 5 samples. The agreement of GFR values between both methods was analyzed with the concordance correlation coefficient (CCC), total deviation index (TDI) and coverage probability (CP). The agreement between both methods was excellent, showing a TDI = 8.1%, which indicates that 90% of the GFR values obtained with DBS showed an error ranging from − 8 to + 8% of the reference method; a CCC of 0.996 (CI: 0.992), reflecting high precision and accuracy and a CP of 94 (CI: 83), indicating that 6% of the GFR values obtained with DBS had an error greater than 10% of the method in blood. So, both methods are interchangeable. DBS represent a major simplification of GFR measurement in mice. Also, DBS improves animal welfare by reducing the total blood required and refining the procedure.Ana Elena Rodríguez-RodríguezSergio Luis-LimaJavier Donate-CorreaLaura Diaz-MartínMaría Rosa ArnauAlejandro Jiménez-SosaFlavio GaspariAlberto OrtizEsteban PorriniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Elena Rodríguez-Rodríguez Sergio Luis-Lima Javier Donate-Correa Laura Diaz-Martín María Rosa Arnau Alejandro Jiménez-Sosa Flavio Gaspari Alberto Ortiz Esteban Porrini Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
description	Abstract There is no simple method to measure glomerular filtration rate (GFR) in mice, which limits the use of mice in models of renal diseases. We aimed at simplifying the plasma clearance of iohexol in mice, using dried blood spot (DBS) sampling in order to reduce the amount of blood taken for analysis. GFR was measured simultaneously by a reference method in total blood—as described before—and tested method using DBS in fifteen male and six female C57BL/6J mice. Total blood extraction was 50 μL for the reference methods and 25μL for the tested methods, distributed in 5 samples. The agreement of GFR values between both methods was analyzed with the concordance correlation coefficient (CCC), total deviation index (TDI) and coverage probability (CP). The agreement between both methods was excellent, showing a TDI = 8.1%, which indicates that 90% of the GFR values obtained with DBS showed an error ranging from − 8 to + 8% of the reference method; a CCC of 0.996 (CI: 0.992), reflecting high precision and accuracy and a CP of 94 (CI: 83), indicating that 6% of the GFR values obtained with DBS had an error greater than 10% of the method in blood. So, both methods are interchangeable. DBS represent a major simplification of GFR measurement in mice. Also, DBS improves animal welfare by reducing the total blood required and refining the procedure.
format	article
author	Ana Elena Rodríguez-Rodríguez Sergio Luis-Lima Javier Donate-Correa Laura Diaz-Martín María Rosa Arnau Alejandro Jiménez-Sosa Flavio Gaspari Alberto Ortiz Esteban Porrini
author_facet	Ana Elena Rodríguez-Rodríguez Sergio Luis-Lima Javier Donate-Correa Laura Diaz-Martín María Rosa Arnau Alejandro Jiménez-Sosa Flavio Gaspari Alberto Ortiz Esteban Porrini
author_sort	Ana Elena Rodríguez-Rodríguez
title	Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
title_short	Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
title_full	Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
title_fullStr	Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
title_full_unstemmed	Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice
title_sort	iohexol plasma clearance simplified by dried blood spot (dbs) sampling to measure renal function in conscious mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/84dec4fe7eb64ad9b3eb968af3079f7c
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Iohexol plasma clearance simplified by Dried Blood Spot (DBS) sampling to measure renal function in conscious mice

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