Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize

Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize

Abstract Maize protein quality is determined by the composition of its endosperm proteins, which are classified as nutritionally poor zeins (prolamin and prolamin-like) and nutritionally rich non-zeins (albumin, globulin, glutelin-like, and glutelin). Protein quality is considerably higher in opaque...

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Autores principales: Mehak Sethi, Alla Singh, Harmanjot Kaur, Ramesh Kumar Phagna, Sujay Rakshit, Dharam Paul Chaudhary
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/84f7296c4b634ab0b9714fa3f26c30f1
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spelling oai:doaj.org-article:84f7296c4b634ab0b9714fa3f26c30f12021-12-02T14:16:16ZExpression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize10.1038/s41598-021-81906-02045-2322https://doaj.org/article/84f7296c4b634ab0b9714fa3f26c30f12021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81906-0https://doaj.org/toc/2045-2322Abstract Maize protein quality is determined by the composition of its endosperm proteins, which are classified as nutritionally poor zeins (prolamin and prolamin-like) and nutritionally rich non-zeins (albumin, globulin, glutelin-like, and glutelin). Protein quality is considerably higher in opaque-2 mutants due to increased content of non-zeins over zeins. However, the opaque-2 endosperm is soft, which leads to poor agronomic performance and post-harvest infestation. Endosperm modification of opaque-2 had led to the development of Quality Protein Maize (QPM), which has higher protein quality along with hard kernel endosperm. The present study was planned to analyze the expression dynamics of different protein fractions in the endospem of developing maize kernel in normal, opaque-2 and QPM in response to the introgression of endosperm modifiers. Results revealed that albumin and globulin content decreases, whereas, prolamin, prolamin-like, glutelin-like, and glutelin content increases with kernel maturity. It has been observed that opaque-2 mutation affects protein expression at initial stages, whereas, the effect of endosperm modifiers was observed at the intermediate and later stages of kernel development. It has also been noted that prolamin, glutelin, and glutelin-like fractions can be used as quick markers for quality assessment for differentiating QPM varieties, even at the immature stage of kernel development. Overall, the present study implicates the role of different protein fractions in developing and utilizing nutritionally improved maize varieties.Mehak SethiAlla SinghHarmanjot KaurRamesh Kumar PhagnaSujay RakshitDharam Paul ChaudharyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Mehak Sethi
Alla Singh
Harmanjot Kaur
Ramesh Kumar Phagna
Sujay Rakshit
Dharam Paul Chaudhary
Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize
description Abstract Maize protein quality is determined by the composition of its endosperm proteins, which are classified as nutritionally poor zeins (prolamin and prolamin-like) and nutritionally rich non-zeins (albumin, globulin, glutelin-like, and glutelin). Protein quality is considerably higher in opaque-2 mutants due to increased content of non-zeins over zeins. However, the opaque-2 endosperm is soft, which leads to poor agronomic performance and post-harvest infestation. Endosperm modification of opaque-2 had led to the development of Quality Protein Maize (QPM), which has higher protein quality along with hard kernel endosperm. The present study was planned to analyze the expression dynamics of different protein fractions in the endospem of developing maize kernel in normal, opaque-2 and QPM in response to the introgression of endosperm modifiers. Results revealed that albumin and globulin content decreases, whereas, prolamin, prolamin-like, glutelin-like, and glutelin content increases with kernel maturity. It has been observed that opaque-2 mutation affects protein expression at initial stages, whereas, the effect of endosperm modifiers was observed at the intermediate and later stages of kernel development. It has also been noted that prolamin, glutelin, and glutelin-like fractions can be used as quick markers for quality assessment for differentiating QPM varieties, even at the immature stage of kernel development. Overall, the present study implicates the role of different protein fractions in developing and utilizing nutritionally improved maize varieties.
format article
author Mehak Sethi
Alla Singh
Harmanjot Kaur
Ramesh Kumar Phagna
Sujay Rakshit
Dharam Paul Chaudhary
author_facet Mehak Sethi
Alla Singh
Harmanjot Kaur
Ramesh Kumar Phagna
Sujay Rakshit
Dharam Paul Chaudhary
author_sort Mehak Sethi
title Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize
title_short Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize
title_full Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize
title_fullStr Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize
title_full_unstemmed Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize
title_sort expression profile of protein fractions in the developing kernel of normal, opaque-2 and quality protein maize
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/84f7296c4b634ab0b9714fa3f26c30f1
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