Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model

A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials – silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/grap...

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Autores principales:	Lužajić Božinovski Tijana, Todorović Vera, Milošević Ivan, Gajdov Vladimir, Prokić Bogomir Bolka, Nešović Katarina, Mišković-Stanković Vesna, Marković Danica
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	immunohistochemistry nanocomposite biomaterials rat skin regeneration Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/84f8543154d8402793eb71408c4f50c4
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spelling	oai:doaj.org-article:84f8543154d8402793eb71408c4f50c42021-11-17T21:27:54ZEvaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model1820-744810.2478/acve-2021-0025https://doaj.org/article/84f8543154d8402793eb71408c4f50c42021-09-01T00:00:00Zhttps://doi.org/10.2478/acve-2021-0025https://doaj.org/toc/1820-7448A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials – silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (α-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue.Lužajić Božinovski TijanaTodorović VeraMilošević IvanGajdov VladimirProkić Bogomir BolkaNešović KatarinaMišković-Stanković VesnaMarković DanicaSciendoarticleimmunohistochemistrynanocomposite biomaterialsratskin regenerationVeterinary medicineSF600-1100ENActa Veterinaria, Vol 71, Iss 3, Pp 285-302 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunohistochemistry nanocomposite biomaterials rat skin regeneration Veterinary medicine SF600-1100
spellingShingle	immunohistochemistry nanocomposite biomaterials rat skin regeneration Veterinary medicine SF600-1100 Lužajić Božinovski Tijana Todorović Vera Milošević Ivan Gajdov Vladimir Prokić Bogomir Bolka Nešović Katarina Mišković-Stanković Vesna Marković Danica Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model
description	A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials – silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (α-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue healing potential and established faster integration and shorter retention in the tissue.
format	article
author	Lužajić Božinovski Tijana Todorović Vera Milošević Ivan Gajdov Vladimir Prokić Bogomir Bolka Nešović Katarina Mišković-Stanković Vesna Marković Danica
author_facet	Lužajić Božinovski Tijana Todorović Vera Milošević Ivan Gajdov Vladimir Prokić Bogomir Bolka Nešović Katarina Mišković-Stanković Vesna Marković Danica
author_sort	Lužajić Božinovski Tijana
title	Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model
title_short	Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model
title_full	Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model
title_fullStr	Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model
title_full_unstemmed	Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model
title_sort	evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/ poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/84f8543154d8402793eb71408c4f50c4
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Evaluation of Soft Tissue Regenerative Processes After Subcutaneous Implantation of Silver/ Poly(Vinyl Alcohol) and Novel Silver/Poly(Vinyl Alcohol)/Graphene Hydrogels in an Animal Model

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