Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles

Abstract Even though application of nanoparticles in medicine seems to provide unique solutions for drug delivery and diagnosis diseases, understanding interactions between nanoscale materials and biological systems is imperative. Therefore, this study determined the effect of different types of nan...

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Autores principales:	Paulina Wigner, Krzysztof Zielinski, Sylwia Michlewska, Paulina Danielska, Agnieszka Marczak, Eduardo Junior Ricci, Ralph Santos-Oliveira, Marzena Szwed
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8509673478214422873f51168337be40
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spelling	oai:doaj.org-article:8509673478214422873f51168337be402021-12-02T10:54:23ZDisturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles10.1038/s41598-021-83291-02045-2322https://doaj.org/article/8509673478214422873f51168337be402021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83291-0https://doaj.org/toc/2045-2322Abstract Even though application of nanoparticles in medicine seems to provide unique solutions for drug delivery and diagnosis diseases, understanding interactions between nanoscale materials and biological systems is imperative. Therefore, this study determined the effect of different types of nanoparticles (NPs) on human endothelial cells and examined the types of toxicity responses they can induce. Four different types of NPs were tested (PLA/MMT/TRASTUZUMAB, PLA/EDTMP, PLGA/MDP, and Pluronic F127 MICELLES), representing three putative areas of application: anticancer therapy, scintigraphy, and cosmetology. The experiments were performed on immortalized human umbilical vein endothelial cells (HUVEC-STs). Light contrast phase microscopy as well as cell viability assays showed that only Pluronic F127 MICELLES decreased the number of HUVEC-STs in contrast to PLA/MMT/TRASTUZUMAB, PLA/EDTMP, and PLGA/MDP NPs, which altered cell morphology, but not their confluency. The tested NPs induced not only DNA strand-breaks and alkali-labile sites, but also internucleosomal DNA fragmentation, visualized as a DNA ladder pattern typical of apoptosis. Moreover, generation of free radicals and subsequent mitochondrial membrane potential collapse showed the significance of free radical production during interactions between NPs and endothelial cells. High concentrations of NPs had different degrees of toxicity in human endothelial cells and affected cell proliferation, redox homeostasis, and triggered mitochondrial dysfunction.Paulina WignerKrzysztof ZielinskiSylwia MichlewskaPaulina DanielskaAgnieszka MarczakEduardo Junior RicciRalph Santos-OliveiraMarzena SzwedNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paulina Wigner Krzysztof Zielinski Sylwia Michlewska Paulina Danielska Agnieszka Marczak Eduardo Junior Ricci Ralph Santos-Oliveira Marzena Szwed Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles
description	Abstract Even though application of nanoparticles in medicine seems to provide unique solutions for drug delivery and diagnosis diseases, understanding interactions between nanoscale materials and biological systems is imperative. Therefore, this study determined the effect of different types of nanoparticles (NPs) on human endothelial cells and examined the types of toxicity responses they can induce. Four different types of NPs were tested (PLA/MMT/TRASTUZUMAB, PLA/EDTMP, PLGA/MDP, and Pluronic F127 MICELLES), representing three putative areas of application: anticancer therapy, scintigraphy, and cosmetology. The experiments were performed on immortalized human umbilical vein endothelial cells (HUVEC-STs). Light contrast phase microscopy as well as cell viability assays showed that only Pluronic F127 MICELLES decreased the number of HUVEC-STs in contrast to PLA/MMT/TRASTUZUMAB, PLA/EDTMP, and PLGA/MDP NPs, which altered cell morphology, but not their confluency. The tested NPs induced not only DNA strand-breaks and alkali-labile sites, but also internucleosomal DNA fragmentation, visualized as a DNA ladder pattern typical of apoptosis. Moreover, generation of free radicals and subsequent mitochondrial membrane potential collapse showed the significance of free radical production during interactions between NPs and endothelial cells. High concentrations of NPs had different degrees of toxicity in human endothelial cells and affected cell proliferation, redox homeostasis, and triggered mitochondrial dysfunction.
format	article
author	Paulina Wigner Krzysztof Zielinski Sylwia Michlewska Paulina Danielska Agnieszka Marczak Eduardo Junior Ricci Ralph Santos-Oliveira Marzena Szwed
author_facet	Paulina Wigner Krzysztof Zielinski Sylwia Michlewska Paulina Danielska Agnieszka Marczak Eduardo Junior Ricci Ralph Santos-Oliveira Marzena Szwed
author_sort	Paulina Wigner
title	Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles
title_short	Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles
title_full	Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles
title_fullStr	Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles
title_full_unstemmed	Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles
title_sort	disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8509673478214422873f51168337be40
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Disturbance of cellular homeostasis as a molecular risk evaluation of human endothelial cells exposed to nanoparticles

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