Imaging the development of chronic Chagas disease after oral transmission

Abstract Chagas disease is a zoonosis caused by the protozoan parasite Trypanosoma cruzi. Transmission cycles are maintained by haematophagous triatomine bug vectors that carry infective T. cruzi in their faeces. Most human infections are acquired by contamination of mucosal membranes with triatomin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Michael D. Lewis, Amanda F. Francisco, Shiromani Jayawardhana, Harry Langston, Martin C. Taylor, John M. Kelly
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/850b2861c2234ed780d6b62cca0fdb24
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:850b2861c2234ed780d6b62cca0fdb24
record_format	dspace
spelling	oai:doaj.org-article:850b2861c2234ed780d6b62cca0fdb242021-12-02T15:07:44ZImaging the development of chronic Chagas disease after oral transmission10.1038/s41598-018-29564-72045-2322https://doaj.org/article/850b2861c2234ed780d6b62cca0fdb242018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29564-7https://doaj.org/toc/2045-2322Abstract Chagas disease is a zoonosis caused by the protozoan parasite Trypanosoma cruzi. Transmission cycles are maintained by haematophagous triatomine bug vectors that carry infective T. cruzi in their faeces. Most human infections are acquired by contamination of mucosal membranes with triatomine faeces after being bitten, however, T. cruzi can be transmitted by several other routes. Oral transmission is an increasingly important aspect of Chagas disease epidemiology, typically involving food or drink products contaminated with triatomines. This has recently caused numerous outbreaks and been linked to unusually severe acute infections. The long-term impact of oral transmission on infection dynamics and disease pathogenesis is unclear. We used highly sensitive bioluminescence imaging and quantitative histopathology to study orally transmitted T. cruzi infections in mice. Both metacyclic and bloodform trypomastigotes were infectious via the oral cavity, but only metacyclics led to established infections by intra-gastric gavage. Mice displayed only mild acute symptoms but later developed significantly increased myocardial collagen content (p = 0.017), indicative of fibrosis. Gastrointestinal tissues and skin were the principal chronic infection reservoirs. Chronic phase parasite load profiles, tissue distribution and myocardial fibrosis severity were comparable to needle-injected controls. Thus, the oral route neither exacerbates nor ameliorates experimental Chagas disease.Michael D. LewisAmanda F. FranciscoShiromani JayawardhanaHarry LangstonMartin C. TaylorJohn M. KellyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael D. Lewis Amanda F. Francisco Shiromani Jayawardhana Harry Langston Martin C. Taylor John M. Kelly Imaging the development of chronic Chagas disease after oral transmission
description	Abstract Chagas disease is a zoonosis caused by the protozoan parasite Trypanosoma cruzi. Transmission cycles are maintained by haematophagous triatomine bug vectors that carry infective T. cruzi in their faeces. Most human infections are acquired by contamination of mucosal membranes with triatomine faeces after being bitten, however, T. cruzi can be transmitted by several other routes. Oral transmission is an increasingly important aspect of Chagas disease epidemiology, typically involving food or drink products contaminated with triatomines. This has recently caused numerous outbreaks and been linked to unusually severe acute infections. The long-term impact of oral transmission on infection dynamics and disease pathogenesis is unclear. We used highly sensitive bioluminescence imaging and quantitative histopathology to study orally transmitted T. cruzi infections in mice. Both metacyclic and bloodform trypomastigotes were infectious via the oral cavity, but only metacyclics led to established infections by intra-gastric gavage. Mice displayed only mild acute symptoms but later developed significantly increased myocardial collagen content (p = 0.017), indicative of fibrosis. Gastrointestinal tissues and skin were the principal chronic infection reservoirs. Chronic phase parasite load profiles, tissue distribution and myocardial fibrosis severity were comparable to needle-injected controls. Thus, the oral route neither exacerbates nor ameliorates experimental Chagas disease.
format	article
author	Michael D. Lewis Amanda F. Francisco Shiromani Jayawardhana Harry Langston Martin C. Taylor John M. Kelly
author_facet	Michael D. Lewis Amanda F. Francisco Shiromani Jayawardhana Harry Langston Martin C. Taylor John M. Kelly
author_sort	Michael D. Lewis
title	Imaging the development of chronic Chagas disease after oral transmission
title_short	Imaging the development of chronic Chagas disease after oral transmission
title_full	Imaging the development of chronic Chagas disease after oral transmission
title_fullStr	Imaging the development of chronic Chagas disease after oral transmission
title_full_unstemmed	Imaging the development of chronic Chagas disease after oral transmission
title_sort	imaging the development of chronic chagas disease after oral transmission
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/850b2861c2234ed780d6b62cca0fdb24
work_keys_str_mv	AT michaeldlewis imagingthedevelopmentofchronicchagasdiseaseafteroraltransmission AT amandaffrancisco imagingthedevelopmentofchronicchagasdiseaseafteroraltransmission AT shiromanijayawardhana imagingthedevelopmentofchronicchagasdiseaseafteroraltransmission AT harrylangston imagingthedevelopmentofchronicchagasdiseaseafteroraltransmission AT martinctaylor imagingthedevelopmentofchronicchagasdiseaseafteroraltransmission AT johnmkelly imagingthedevelopmentofchronicchagasdiseaseafteroraltransmission
_version_	1718388400118038528

Imaging the development of chronic Chagas disease after oral transmission

Ejemplares similares