bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway

Abstract Burn injuries are most challenging to manage since it causes loss of the integrity of large portions of the skin leading to major disability or even death. Over the years, hydrogels are considered as a significant delivery system for wound treatment because of several advantages over other...

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Autores principales:	Srijita Chakrabarti, Bhaskar Mazumder, Jadab Rajkonwar, Manash Pratim Pathak, Pompy Patowary, Pronobesh Chattopadhyay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/850f6ef9e32d459b98d798a925007988
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spelling	oai:doaj.org-article:850f6ef9e32d459b98d798a9250079882021-12-02T13:30:09ZbFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway10.1038/s41598-021-82888-92045-2322https://doaj.org/article/850f6ef9e32d459b98d798a9250079882021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82888-9https://doaj.org/toc/2045-2322Abstract Burn injuries are most challenging to manage since it causes loss of the integrity of large portions of the skin leading to major disability or even death. Over the years, hydrogels are considered as a significant delivery system for wound treatment because of several advantages over other conventional formulations. We hypothesized that the bFGF-collagen-AgSD incorporated hydrogel formulation can accelerate the rate of burn healing in animal model and would promote fibroblast cell proliferation. Neovascularization and re-epithelialization is a hall mark of burn wound healing. In the present study, histopathological investigation and scanning electron microscopy of skin tissue of Wistar rats showed almost complete epithelialisation after 16 days in the treatment group. The developed hydrogel showed significantly accelerated wound closure compared with a standard and control group. The faster wound closure resulted from increased re-epithelialization and granulation tissue formation because of the presence of collagen and growth factor. Expressions of proteins such as TrkA, p- TrkA, ERK1/2, p-ERK1/2, NF-kβ, and p-NF-kβ involved in nerve growth factor (NGF) signalling pathway were analysed by western blot. All the findings obtained from this study indicated that the hydrogel can be considered as a promising delivery system against second degree burn by faster healing.Srijita ChakrabartiBhaskar MazumderJadab RajkonwarManash Pratim PathakPompy PatowaryPronobesh ChattopadhyayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Srijita Chakrabarti Bhaskar Mazumder Jadab Rajkonwar Manash Pratim Pathak Pompy Patowary Pronobesh Chattopadhyay bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway
description	Abstract Burn injuries are most challenging to manage since it causes loss of the integrity of large portions of the skin leading to major disability or even death. Over the years, hydrogels are considered as a significant delivery system for wound treatment because of several advantages over other conventional formulations. We hypothesized that the bFGF-collagen-AgSD incorporated hydrogel formulation can accelerate the rate of burn healing in animal model and would promote fibroblast cell proliferation. Neovascularization and re-epithelialization is a hall mark of burn wound healing. In the present study, histopathological investigation and scanning electron microscopy of skin tissue of Wistar rats showed almost complete epithelialisation after 16 days in the treatment group. The developed hydrogel showed significantly accelerated wound closure compared with a standard and control group. The faster wound closure resulted from increased re-epithelialization and granulation tissue formation because of the presence of collagen and growth factor. Expressions of proteins such as TrkA, p- TrkA, ERK1/2, p-ERK1/2, NF-kβ, and p-NF-kβ involved in nerve growth factor (NGF) signalling pathway were analysed by western blot. All the findings obtained from this study indicated that the hydrogel can be considered as a promising delivery system against second degree burn by faster healing.
format	article
author	Srijita Chakrabarti Bhaskar Mazumder Jadab Rajkonwar Manash Pratim Pathak Pompy Patowary Pronobesh Chattopadhyay
author_facet	Srijita Chakrabarti Bhaskar Mazumder Jadab Rajkonwar Manash Pratim Pathak Pompy Patowary Pronobesh Chattopadhyay
author_sort	Srijita Chakrabarti
title	bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway
title_short	bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway
title_full	bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway
title_fullStr	bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway
title_full_unstemmed	bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway
title_sort	bfgf and collagen matrix hydrogel attenuates burn wound inflammation through activation of erk and trk pathway
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/850f6ef9e32d459b98d798a925007988
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bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway

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