INTRALABORATORY VALIDATION OF AN ANALYTICAL METHODOLOGY BY UV/VIS SPECTROMETRY IN ORDER TO EVALUATE THE OCULAR IRRITANT POTENTIAL OF TENSOACTIVES IN RED BLOOD CELLS

Red blood cell assay (RBC) intralaboratory validation was presented, specifically for linearity, repeatability and intermediate precision. RBC is an in vitro methodology employed for ocular irritation evaluation measuring surfactants adverse effects on cell membranes and proteins and is an alternati...

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Autores principales:	Luz Adriana LEGUIZAMÓN, Ever Andrés HERRERA, Jorge Ariel MARTÍNEZ, María C. LOZANO
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2011
Materias:	Sodium dodecylsulphate surfactants hemolysis validation studies animal testing alternatives Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/8513b0f82d3046d2a890440061d0e794
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spelling	oai:doaj.org-article:8513b0f82d3046d2a890440061d0e7942021-11-19T04:14:07ZINTRALABORATORY VALIDATION OF AN ANALYTICAL METHODOLOGY BY UV/VIS SPECTROMETRY IN ORDER TO EVALUATE THE OCULAR IRRITANT POTENTIAL OF TENSOACTIVES IN RED BLOOD CELLS0121-40042145-2660https://doaj.org/article/8513b0f82d3046d2a890440061d0e7942011-05-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/8777https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660Red blood cell assay (RBC) intralaboratory validation was presented, specifically for linearity, repeatability and intermediate precision. RBC is an in vitro methodology employed for ocular irritation evaluation measuring surfactants adverse effects on cell membranes and proteins and is an alternative for Draize ocular test. Free hemoglobin after red blood cell lysis was determined through spectrometry at 577 nm in order to estimate medium hemolytic concentration (HC50) indicating cell damage by surfactant. Also hemoglobin denaturation induced by high concentrations of surfactants was considered measuring denaturation index (DI) through absorbance ratio at 542 and 577 determinations. HC50 / DI is an indicator of the dodecylsulphate (SDS) irritant potential, surfactant used in order to validate this assay. Parameters previously mentioned were validated and we present an easy and reliable alternative methodology for animal experimentation to assess surfactants ocular irritation for SDS.Luz Adriana LEGUIZAMÓNEver Andrés HERRERAJorge Ariel MARTÍNEZMaría C. LOZANOUniversidad de AntioquiaarticleSodium dodecylsulphatesurfactantshemolysisvalidation studiesanimal testing alternativesFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 18, Iss 1 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sodium dodecylsulphate surfactants hemolysis validation studies animal testing alternatives Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Sodium dodecylsulphate surfactants hemolysis validation studies animal testing alternatives Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Luz Adriana LEGUIZAMÓN Ever Andrés HERRERA Jorge Ariel MARTÍNEZ María C. LOZANO INTRALABORATORY VALIDATION OF AN ANALYTICAL METHODOLOGY BY UV/VIS SPECTROMETRY IN ORDER TO EVALUATE THE OCULAR IRRITANT POTENTIAL OF TENSOACTIVES IN RED BLOOD CELLS
description	Red blood cell assay (RBC) intralaboratory validation was presented, specifically for linearity, repeatability and intermediate precision. RBC is an in vitro methodology employed for ocular irritation evaluation measuring surfactants adverse effects on cell membranes and proteins and is an alternative for Draize ocular test. Free hemoglobin after red blood cell lysis was determined through spectrometry at 577 nm in order to estimate medium hemolytic concentration (HC50) indicating cell damage by surfactant. Also hemoglobin denaturation induced by high concentrations of surfactants was considered measuring denaturation index (DI) through absorbance ratio at 542 and 577 determinations. HC50 / DI is an indicator of the dodecylsulphate (SDS) irritant potential, surfactant used in order to validate this assay. Parameters previously mentioned were validated and we present an easy and reliable alternative methodology for animal experimentation to assess surfactants ocular irritation for SDS.
format	article
author	Luz Adriana LEGUIZAMÓN Ever Andrés HERRERA Jorge Ariel MARTÍNEZ María C. LOZANO
author_facet	Luz Adriana LEGUIZAMÓN Ever Andrés HERRERA Jorge Ariel MARTÍNEZ María C. LOZANO
author_sort	Luz Adriana LEGUIZAMÓN
title	INTRALABORATORY VALIDATION OF AN ANALYTICAL METHODOLOGY BY UV/VIS SPECTROMETRY IN ORDER TO EVALUATE THE OCULAR IRRITANT POTENTIAL OF TENSOACTIVES IN RED BLOOD CELLS
title_short	INTRALABORATORY VALIDATION OF AN ANALYTICAL METHODOLOGY BY UV/VIS SPECTROMETRY IN ORDER TO EVALUATE THE OCULAR IRRITANT POTENTIAL OF TENSOACTIVES IN RED BLOOD CELLS
title_full	INTRALABORATORY VALIDATION OF AN ANALYTICAL METHODOLOGY BY UV/VIS SPECTROMETRY IN ORDER TO EVALUATE THE OCULAR IRRITANT POTENTIAL OF TENSOACTIVES IN RED BLOOD CELLS
title_fullStr	INTRALABORATORY VALIDATION OF AN ANALYTICAL METHODOLOGY BY UV/VIS SPECTROMETRY IN ORDER TO EVALUATE THE OCULAR IRRITANT POTENTIAL OF TENSOACTIVES IN RED BLOOD CELLS
title_full_unstemmed	INTRALABORATORY VALIDATION OF AN ANALYTICAL METHODOLOGY BY UV/VIS SPECTROMETRY IN ORDER TO EVALUATE THE OCULAR IRRITANT POTENTIAL OF TENSOACTIVES IN RED BLOOD CELLS
title_sort	intralaboratory validation of an analytical methodology by uv/vis spectrometry in order to evaluate the ocular irritant potential of tensoactives in red blood cells
publisher	Universidad de Antioquia
publishDate	2011
url	https://doaj.org/article/8513b0f82d3046d2a890440061d0e794
work_keys_str_mv	AT luzadrianaleguizamon intralaboratoryvalidationofananalyticalmethodologybyuvvisspectrometryinordertoevaluatetheocularirritantpotentialoftensoactivesinredbloodcells AT everandresherrera intralaboratoryvalidationofananalyticalmethodologybyuvvisspectrometryinordertoevaluatetheocularirritantpotentialoftensoactivesinredbloodcells AT jorgearielmartinez intralaboratoryvalidationofananalyticalmethodologybyuvvisspectrometryinordertoevaluatetheocularirritantpotentialoftensoactivesinredbloodcells AT mariaclozano intralaboratoryvalidationofananalyticalmethodologybyuvvisspectrometryinordertoevaluatetheocularirritantpotentialoftensoactivesinredbloodcells
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INTRALABORATORY VALIDATION OF AN ANALYTICAL METHODOLOGY BY UV/VIS SPECTROMETRY IN ORDER TO EVALUATE THE OCULAR IRRITANT POTENTIAL OF TENSOACTIVES IN RED BLOOD CELLS

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