Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing

Abstract Inspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblas...

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Autores principales:	Jeong Yu Lee, Dae-Jin Min, Wanil Kim, Bum-Ho Bin, Kyuhan Kim, Eun-Gyung Cho
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8517c21511cf466c8809c614580d5ba5
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spelling	oai:doaj.org-article:8517c21511cf466c8809c614580d5ba52021-12-02T14:16:34ZNon pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing10.1038/s41598-021-81878-12045-2322https://doaj.org/article/8517c21511cf466c8809c614580d5ba52021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81878-1https://doaj.org/toc/2045-2322Abstract Inspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short-term ultrasound of various amplitudes significantly increased the proliferation and migration of fibroblasts and subsequently increased the production of the extracellular matrix components fibronectin and collagen type I, both of which are important for wound healing and are secreted by fibroblasts. In addition, ultrasound treatment increased the contraction of a fibroblast-embedded three-dimensional collagen matrix, and the effect was synergistically increased in the presence of TGF-β. RNA-sequencing and bioinformatics analyses revealed changes in gene expression and p38 and ERK1/2 MAPK pathway activation in the ultrasound-stimulated fibroblasts. Our findings suggest that ultrasound as a mechanical stimulus can activate human dermal fibroblasts. Therefore, the activation of fibroblasts using ultrasound may improve the healing of various types of wounds and increase skin regeneration.Jeong Yu LeeDae-Jin MinWanil KimBum-Ho BinKyuhan KimEun-Gyung ChoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeong Yu Lee Dae-Jin Min Wanil Kim Bum-Ho Bin Kyuhan Kim Eun-Gyung Cho Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
description	Abstract Inspired by the effectiveness of low-intensity ultrasound on tissue regeneration, we investigated the potential effect of short-term high-intensity ultrasound treatment for acceleration of wound healing in an in vitro wound model and dermal equivalent, both comprising human dermal fibroblasts. Short-term ultrasound of various amplitudes significantly increased the proliferation and migration of fibroblasts and subsequently increased the production of the extracellular matrix components fibronectin and collagen type I, both of which are important for wound healing and are secreted by fibroblasts. In addition, ultrasound treatment increased the contraction of a fibroblast-embedded three-dimensional collagen matrix, and the effect was synergistically increased in the presence of TGF-β. RNA-sequencing and bioinformatics analyses revealed changes in gene expression and p38 and ERK1/2 MAPK pathway activation in the ultrasound-stimulated fibroblasts. Our findings suggest that ultrasound as a mechanical stimulus can activate human dermal fibroblasts. Therefore, the activation of fibroblasts using ultrasound may improve the healing of various types of wounds and increase skin regeneration.
format	article
author	Jeong Yu Lee Dae-Jin Min Wanil Kim Bum-Ho Bin Kyuhan Kim Eun-Gyung Cho
author_facet	Jeong Yu Lee Dae-Jin Min Wanil Kim Bum-Ho Bin Kyuhan Kim Eun-Gyung Cho
author_sort	Jeong Yu Lee
title	Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title_short	Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title_full	Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title_fullStr	Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title_full_unstemmed	Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
title_sort	non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8517c21511cf466c8809c614580d5ba5
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Non pharmacological high-intensity ultrasound treatment of human dermal fibroblasts to accelerate wound healing

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