Effects of long-term exercise training on adipose tissue expression of fractalkine and MCP-1 in patients with type 2 diabetes and stable coronary artery disease: a substudy of a randomized controlled trial
Ida Unhammer Njerve,1–3 Rune Byrkjeland,1–3 Harald Arnesen,1–3 Sissel Åkra,1,2 Svein Solheim,1,2 Ingebjørg Seljeflot1–3 1Department of Cardiology, Center for Clinical Heart Research, Oslo University Hospital, Ullevål, 2Center for...
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Dove Medical Press
2016
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oai:doaj.org-article:8519df227c15438c9eb082894fca5c4e2021-12-02T00:05:29ZEffects of long-term exercise training on adipose tissue expression of fractalkine and MCP-1 in patients with type 2 diabetes and stable coronary artery disease: a substudy of a randomized controlled trial1178-7007https://doaj.org/article/8519df227c15438c9eb082894fca5c4e2016-03-01T00:00:00Zhttps://www.dovepress.com/effects-of-long-term-exercise-training-on-adipose-tissue-expression-of-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Ida Unhammer Njerve,1–3 Rune Byrkjeland,1–3 Harald Arnesen,1–3 Sissel Åkra,1,2 Svein Solheim,1,2 Ingebjørg Seljeflot1–3 1Department of Cardiology, Center for Clinical Heart Research, Oslo University Hospital, Ullevål, 2Center for Heart Failure Research, Oslo University Hospital, 3Faculty of Medicine, University of Oslo, Oslo, Norway Purpose: Adipose tissue inflammation plays a role in atherosclerosis and type 2 diabetes (T2DM). We aimed to investigate whether 12 months of exercise training in patients with both T2DM and coronary artery disease (CAD) reduced the genetic expression of the proinflammatory markers fractalkine (CX3CL1) and its receptor (CX3CR1) and monocyte chemoattractant protein-1 (MCP-1) in the subcutaneous adipose tissue. Expression of the genes in the circulating leukocytes and circulating levels of the markers were also investigated. Patients and methods: A total of 137 patients with T2DM and CAD were included to study the effects of exercise on atherosclerosis progression and glucose control. Patients were randomized to exercise training (combined aerobic and strength training) or control. At inclusion and after 12 months, fasting blood samples and a subcutaneous adipose tissue sample were taken. RNA was extracted from the adipose tissue and circulating leukocytes, and the expression levels were examined by reverse transcription-polymerase chain reaction. Circulating fractalkine and MCP-1 were determined by enzyme-linked immunosorbent assay. Results: The analyses were performed in 114 patients who completed the study and adhered to the intervention principle. At baseline, gene expression of fractalkine and CX3CR1 in the adipose tissue was similar in the two groups. There were no change within either group and no between-group differences in changes from baseline. Circulating fractalkine increased after 12 months in the exercise group (P=0.044), significantly more compared to controls (P=0.042), however only in the patients with advanced vascular disease. Neither the expression levels of MCP-1 nor the circulating levels changed significantly in either group. At baseline, CX3CR1 expression in the adipose tissue was associated with body mass index (P<0.001). Conclusion: No significant effects of long-term exercise training on adipose tissue expression of fractalkine, CX3CR1, or MCP-1 were found in our patients with combined CAD and T2DM. However, a slight increase in circulating fractalkine after the intervention was recorded. The association of CX3CR1 expression with body mass index might indicate increased immune activation in the adipose tissue. Keywords: diabetes, coronary artery disease, subcutaneous adipose tissue, exercise, MCP-1, fractalkine (CX3CL1)Njerve IUByrkjelRArnesen HÅkra SSolheim SSeljeflot IDove Medical PressarticleDiabetesCoronary artery diseaseSubcutaneous adipose tissueExerciseMCP-1Fractalkine (CX3CL1)Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol 2016, Iss Issue 1, Pp 55-62 (2016) |
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Diabetes Coronary artery disease Subcutaneous adipose tissue Exercise MCP-1 Fractalkine (CX3CL1) Specialties of internal medicine RC581-951 |
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Diabetes Coronary artery disease Subcutaneous adipose tissue Exercise MCP-1 Fractalkine (CX3CL1) Specialties of internal medicine RC581-951 Njerve IU Byrkjel R Arnesen H Åkra S Solheim S Seljeflot I Effects of long-term exercise training on adipose tissue expression of fractalkine and MCP-1 in patients with type 2 diabetes and stable coronary artery disease: a substudy of a randomized controlled trial |
| description |
Ida Unhammer Njerve,1–3 Rune Byrkjeland,1–3 Harald Arnesen,1–3 Sissel Åkra,1,2 Svein Solheim,1,2 Ingebjørg Seljeflot1–3 1Department of Cardiology, Center for Clinical Heart Research, Oslo University Hospital, Ullevål, 2Center for Heart Failure Research, Oslo University Hospital, 3Faculty of Medicine, University of Oslo, Oslo, Norway Purpose: Adipose tissue inflammation plays a role in atherosclerosis and type 2 diabetes (T2DM). We aimed to investigate whether 12 months of exercise training in patients with both T2DM and coronary artery disease (CAD) reduced the genetic expression of the proinflammatory markers fractalkine (CX3CL1) and its receptor (CX3CR1) and monocyte chemoattractant protein-1 (MCP-1) in the subcutaneous adipose tissue. Expression of the genes in the circulating leukocytes and circulating levels of the markers were also investigated. Patients and methods: A total of 137 patients with T2DM and CAD were included to study the effects of exercise on atherosclerosis progression and glucose control. Patients were randomized to exercise training (combined aerobic and strength training) or control. At inclusion and after 12 months, fasting blood samples and a subcutaneous adipose tissue sample were taken. RNA was extracted from the adipose tissue and circulating leukocytes, and the expression levels were examined by reverse transcription-polymerase chain reaction. Circulating fractalkine and MCP-1 were determined by enzyme-linked immunosorbent assay. Results: The analyses were performed in 114 patients who completed the study and adhered to the intervention principle. At baseline, gene expression of fractalkine and CX3CR1 in the adipose tissue was similar in the two groups. There were no change within either group and no between-group differences in changes from baseline. Circulating fractalkine increased after 12 months in the exercise group (P=0.044), significantly more compared to controls (P=0.042), however only in the patients with advanced vascular disease. Neither the expression levels of MCP-1 nor the circulating levels changed significantly in either group. At baseline, CX3CR1 expression in the adipose tissue was associated with body mass index (P<0.001). Conclusion: No significant effects of long-term exercise training on adipose tissue expression of fractalkine, CX3CR1, or MCP-1 were found in our patients with combined CAD and T2DM. However, a slight increase in circulating fractalkine after the intervention was recorded. The association of CX3CR1 expression with body mass index might indicate increased immune activation in the adipose tissue. Keywords: diabetes, coronary artery disease, subcutaneous adipose tissue, exercise, MCP-1, fractalkine (CX3CL1) |
| format |
article |
| author |
Njerve IU Byrkjel R Arnesen H Åkra S Solheim S Seljeflot I |
| author_facet |
Njerve IU Byrkjel R Arnesen H Åkra S Solheim S Seljeflot I |
| author_sort |
Njerve IU |
| title |
Effects of long-term exercise training on adipose tissue expression of fractalkine and MCP-1 in patients with type 2 diabetes and stable coronary artery disease: a substudy of a randomized controlled trial |
| title_short |
Effects of long-term exercise training on adipose tissue expression of fractalkine and MCP-1 in patients with type 2 diabetes and stable coronary artery disease: a substudy of a randomized controlled trial |
| title_full |
Effects of long-term exercise training on adipose tissue expression of fractalkine and MCP-1 in patients with type 2 diabetes and stable coronary artery disease: a substudy of a randomized controlled trial |
| title_fullStr |
Effects of long-term exercise training on adipose tissue expression of fractalkine and MCP-1 in patients with type 2 diabetes and stable coronary artery disease: a substudy of a randomized controlled trial |
| title_full_unstemmed |
Effects of long-term exercise training on adipose tissue expression of fractalkine and MCP-1 in patients with type 2 diabetes and stable coronary artery disease: a substudy of a randomized controlled trial |
| title_sort |
effects of long-term exercise training on adipose tissue expression of fractalkine and mcp-1 in patients with type 2 diabetes and stable coronary artery disease: a substudy of a randomized controlled trial |
| publisher |
Dove Medical Press |
| publishDate |
2016 |
| url |
https://doaj.org/article/8519df227c15438c9eb082894fca5c4e |
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