<italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Vivo</italic> Studies of a Rapid and Selective Breath Test for Tuberculosis Based upon Mycobacterial CO Dehydrogenase

ABSTRACT One of the major hurdles in treating tuberculosis (TB) is the time-consuming and difficult methodology for diagnosis. Stable-isotope breath tests hold great potential for rapidly diagnosing an infectious disease, monitoring therapy, and determining a bacterial phenotype in a rapid, point-of...

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Autores principales:	Mamoudou Maiga, Seong Won Choi, Viorel Atudorei, Mariama C. Maiga, Zachary D. Sharp, William R. Bishai, Graham S. Timmins
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/851a2229eb62494c94bb4c36f2be6d40
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spelling	oai:doaj.org-article:851a2229eb62494c94bb4c36f2be6d402021-11-15T15:45:12Z<italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Vivo</italic> Studies of a Rapid and Selective Breath Test for Tuberculosis Based upon Mycobacterial CO Dehydrogenase10.1128/mBio.00990-142150-7511https://doaj.org/article/851a2229eb62494c94bb4c36f2be6d402014-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00990-14https://doaj.org/toc/2150-7511ABSTRACT One of the major hurdles in treating tuberculosis (TB) is the time-consuming and difficult methodology for diagnosis. Stable-isotope breath tests hold great potential for rapidly diagnosing an infectious disease, monitoring therapy, and determining a bacterial phenotype in a rapid, point-of-care manner that does not require invasive sampling. Here we describe the preclinical development of a potentially highly selective TB diagnostic breath test based upon the organism’s CO dehydrogenase activity. After development of the test in vitro, we were able to use the breath test to discriminate between infected and control rabbits, demonstrating that a diagnosis can potentially be made and also that a complex bacterial phenotype can be noninvasively and rapidly studied in the host. IMPORTANCE Tuberculosis (TB) remains a major infectious cause of disease and death worldwide, and effective diagnosis and then treatment are the tools with which we fight TB. The more quickly and more specific the diagnosis can be made, the better, and this is also true of diagnosis being as close to the patient (point of care) as possible. Here we report our preclinical development of breath tests based upon specific mycobacterial metabolism that could, with development, allow rapid point-of-care diagnosis through measuring the mycobacterial conversion of labeled CO to labeled CO2.Mamoudou MaigaSeong Won ChoiViorel AtudoreiMariama C. MaigaZachary D. SharpWilliam R. BishaiGraham S. TimminsAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 2 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Mamoudou Maiga Seong Won Choi Viorel Atudorei Mariama C. Maiga Zachary D. Sharp William R. Bishai Graham S. Timmins <italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Vivo</italic> Studies of a Rapid and Selective Breath Test for Tuberculosis Based upon Mycobacterial CO Dehydrogenase
description	ABSTRACT One of the major hurdles in treating tuberculosis (TB) is the time-consuming and difficult methodology for diagnosis. Stable-isotope breath tests hold great potential for rapidly diagnosing an infectious disease, monitoring therapy, and determining a bacterial phenotype in a rapid, point-of-care manner that does not require invasive sampling. Here we describe the preclinical development of a potentially highly selective TB diagnostic breath test based upon the organism’s CO dehydrogenase activity. After development of the test in vitro, we were able to use the breath test to discriminate between infected and control rabbits, demonstrating that a diagnosis can potentially be made and also that a complex bacterial phenotype can be noninvasively and rapidly studied in the host. IMPORTANCE Tuberculosis (TB) remains a major infectious cause of disease and death worldwide, and effective diagnosis and then treatment are the tools with which we fight TB. The more quickly and more specific the diagnosis can be made, the better, and this is also true of diagnosis being as close to the patient (point of care) as possible. Here we report our preclinical development of breath tests based upon specific mycobacterial metabolism that could, with development, allow rapid point-of-care diagnosis through measuring the mycobacterial conversion of labeled CO to labeled CO2.
format	article
author	Mamoudou Maiga Seong Won Choi Viorel Atudorei Mariama C. Maiga Zachary D. Sharp William R. Bishai Graham S. Timmins
author_facet	Mamoudou Maiga Seong Won Choi Viorel Atudorei Mariama C. Maiga Zachary D. Sharp William R. Bishai Graham S. Timmins
author_sort	Mamoudou Maiga
title	<italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Vivo</italic> Studies of a Rapid and Selective Breath Test for Tuberculosis Based upon Mycobacterial CO Dehydrogenase
title_short	<italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Vivo</italic> Studies of a Rapid and Selective Breath Test for Tuberculosis Based upon Mycobacterial CO Dehydrogenase
title_full	<italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Vivo</italic> Studies of a Rapid and Selective Breath Test for Tuberculosis Based upon Mycobacterial CO Dehydrogenase
title_fullStr	<italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Vivo</italic> Studies of a Rapid and Selective Breath Test for Tuberculosis Based upon Mycobacterial CO Dehydrogenase
title_full_unstemmed	<italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Vivo</italic> Studies of a Rapid and Selective Breath Test for Tuberculosis Based upon Mycobacterial CO Dehydrogenase
title_sort	<italic toggle="yes">in vitro</italic> and <italic toggle="yes">in vivo</italic> studies of a rapid and selective breath test for tuberculosis based upon mycobacterial co dehydrogenase
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/851a2229eb62494c94bb4c36f2be6d40
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<italic toggle="yes">In Vitro</italic> and <italic toggle="yes">In Vivo</italic> Studies of a Rapid and Selective Breath Test for Tuberculosis Based upon Mycobacterial CO Dehydrogenase

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