Aging, estrogen loss and epoxyeicosatrienoic acids (EETs).

Inflammation is a key element in many cardiovascular diseases. Both estrogen loss, caused by menopause, and aging have inflammatory consequences. Epoxyeicosatrienoic acids (EETs) are anti-inflammatory molecules synthesized by various cytochrome P450 (Cyp) enzymes from arachidonic acid. EETs are in t...

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Autores principales:	Alison R Lee, Angela S Pechenino, Hua Dong, Bruce D Hammock, Anne A Knowlton
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8520dc3d24a64317af40b2fa3eda9ffe
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spelling	oai:doaj.org-article:8520dc3d24a64317af40b2fa3eda9ffe2021-11-18T08:59:58ZAging, estrogen loss and epoxyeicosatrienoic acids (EETs).1932-620310.1371/journal.pone.0070719https://doaj.org/article/8520dc3d24a64317af40b2fa3eda9ffe2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23967089/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Inflammation is a key element in many cardiovascular diseases. Both estrogen loss, caused by menopause, and aging have inflammatory consequences. Epoxyeicosatrienoic acids (EETs) are anti-inflammatory molecules synthesized by various cytochrome P450 (Cyp) enzymes from arachidonic acid. EETs are in the third (Cytochrome P450) pathway of arachindonic acid metabolism, others being cyclooxygenases and lipoxygenases. We hypothesized that aging and estrogen loss would reduce levels of anti-inflammatory EETs. Adult (6 mo) and aged (22 mo) ovariectomized rats with (OP) and without (Ovx) 17-∃-estradiol replacement were used in this study. Mass spectrometry was used to measure levels of EETs and their metabolites, dihydroxyeicosatrienoic acids (DHETs). Levels of Cyp2C2, Cyp2C6, and Cyp2J2, the principal Cyps responsible for EETs synthesis, as well as soluble epoxide hydrolase (sEH), which metabolizes EETS to DHETs, were determined via western blot. Overall Cyp levels decreased with age, though Cyp2C6 increased in the liver. sEH was increased in the kidney with estrogen replacement. Despite protein changes, no differences were measured in plasma or aortic tissue levels of EETs. However, plasma 14,15 DHET was increased in aged Ovx, and 5,6 DHET in adult OP. In conclusion neither aging nor estrogen loss decreased the anti-inflammatory EETs in the cardiovascular system.Alison R LeeAngela S PecheninoHua DongBruce D HammockAnne A KnowltonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e70719 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alison R Lee Angela S Pechenino Hua Dong Bruce D Hammock Anne A Knowlton Aging, estrogen loss and epoxyeicosatrienoic acids (EETs).
description	Inflammation is a key element in many cardiovascular diseases. Both estrogen loss, caused by menopause, and aging have inflammatory consequences. Epoxyeicosatrienoic acids (EETs) are anti-inflammatory molecules synthesized by various cytochrome P450 (Cyp) enzymes from arachidonic acid. EETs are in the third (Cytochrome P450) pathway of arachindonic acid metabolism, others being cyclooxygenases and lipoxygenases. We hypothesized that aging and estrogen loss would reduce levels of anti-inflammatory EETs. Adult (6 mo) and aged (22 mo) ovariectomized rats with (OP) and without (Ovx) 17-∃-estradiol replacement were used in this study. Mass spectrometry was used to measure levels of EETs and their metabolites, dihydroxyeicosatrienoic acids (DHETs). Levels of Cyp2C2, Cyp2C6, and Cyp2J2, the principal Cyps responsible for EETs synthesis, as well as soluble epoxide hydrolase (sEH), which metabolizes EETS to DHETs, were determined via western blot. Overall Cyp levels decreased with age, though Cyp2C6 increased in the liver. sEH was increased in the kidney with estrogen replacement. Despite protein changes, no differences were measured in plasma or aortic tissue levels of EETs. However, plasma 14,15 DHET was increased in aged Ovx, and 5,6 DHET in adult OP. In conclusion neither aging nor estrogen loss decreased the anti-inflammatory EETs in the cardiovascular system.
format	article
author	Alison R Lee Angela S Pechenino Hua Dong Bruce D Hammock Anne A Knowlton
author_facet	Alison R Lee Angela S Pechenino Hua Dong Bruce D Hammock Anne A Knowlton
author_sort	Alison R Lee
title	Aging, estrogen loss and epoxyeicosatrienoic acids (EETs).
title_short	Aging, estrogen loss and epoxyeicosatrienoic acids (EETs).
title_full	Aging, estrogen loss and epoxyeicosatrienoic acids (EETs).
title_fullStr	Aging, estrogen loss and epoxyeicosatrienoic acids (EETs).
title_full_unstemmed	Aging, estrogen loss and epoxyeicosatrienoic acids (EETs).
title_sort	aging, estrogen loss and epoxyeicosatrienoic acids (eets).
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8520dc3d24a64317af40b2fa3eda9ffe
work_keys_str_mv	AT alisonrlee agingestrogenlossandepoxyeicosatrienoicacidseets AT angelaspechenino agingestrogenlossandepoxyeicosatrienoicacidseets AT huadong agingestrogenlossandepoxyeicosatrienoicacidseets AT brucedhammock agingestrogenlossandepoxyeicosatrienoicacidseets AT anneaknowlton agingestrogenlossandepoxyeicosatrienoicacidseets
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Aging, estrogen loss and epoxyeicosatrienoic acids (EETs).

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