Genomic instability of I elements of Drosophila melanogaster in absence of dysgenic crosses.

Retrotranspostion of I factors in the female germline of Drosophila melanogaster is responsible for the so called I-R hybrid dysgenesis, a phenomenon that produces a broad spectrum of genetic abnormalities including reduced fertility, increased frequency of mutations and chromosome loss. Transpositi...

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Autores principales:	Roberta Moschetti, Patrizio Dimitri, Ruggiero Caizzi, Nikolaj Junakovic
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8525f6a625a44519a0e006be8e8010c8
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spelling	oai:doaj.org-article:8525f6a625a44519a0e006be8e8010c82021-11-18T07:03:42ZGenomic instability of I elements of Drosophila melanogaster in absence of dysgenic crosses.1932-620310.1371/journal.pone.0013142https://doaj.org/article/8525f6a625a44519a0e006be8e8010c82010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20957225/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Retrotranspostion of I factors in the female germline of Drosophila melanogaster is responsible for the so called I-R hybrid dysgenesis, a phenomenon that produces a broad spectrum of genetic abnormalities including reduced fertility, increased frequency of mutations and chromosome loss. Transposition of I factor depends on cellular conditions that are established in the oocytes of the reactive females and transmitted to their daughters. The so-called reactivity is a cellular state that may exhibit variable levels of expression and represents a permissive condition for I transposition at high levels. Defective I elements have been proposed to be the genetic determinants of reactivity and, through their differential expression, to modulate transposition of active copies in somatic and/or germ line cells. Recently, control of transposable element activity in the germ line has been found to depend on pi-RNAs, small repressive RNAs interacting with Piwi-family proteins and derived from larger transposable elements (TE)-derived primary transcripts. In particular, maternally transmitted I-element piRNAs originating from the 42AB region of polytene chromosomes were found to be involved in control of I element mobility. In the present work, we use a combination of cytological and molecular approaches to study the activity of I elements in three sublines of the inducer y; cn bw; sp isogenic strain and in dysgenic and non-dysgenic genetic backgrounds. Overall, the results of FISH and Southern blotting experiments clearly show that I elements are highly unstable in the Montpellier subline in the absence of classical dysgenic conditions. Such instability appears to be correlated to the amount of 5' and 3' I element transcripts detected by quantitative and real-time RT-PCR. The results of this study indicate that I elements can be highly active in the absence of a dysgenic crosses. Moreover, in the light of our results caution should be taken to assimilate the genomic annotation data on transposable elements to all y; cn bw sp sublines.Roberta MoschettiPatrizio DimitriRuggiero CaizziNikolaj JunakovicPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roberta Moschetti Patrizio Dimitri Ruggiero Caizzi Nikolaj Junakovic Genomic instability of I elements of Drosophila melanogaster in absence of dysgenic crosses.
description	Retrotranspostion of I factors in the female germline of Drosophila melanogaster is responsible for the so called I-R hybrid dysgenesis, a phenomenon that produces a broad spectrum of genetic abnormalities including reduced fertility, increased frequency of mutations and chromosome loss. Transposition of I factor depends on cellular conditions that are established in the oocytes of the reactive females and transmitted to their daughters. The so-called reactivity is a cellular state that may exhibit variable levels of expression and represents a permissive condition for I transposition at high levels. Defective I elements have been proposed to be the genetic determinants of reactivity and, through their differential expression, to modulate transposition of active copies in somatic and/or germ line cells. Recently, control of transposable element activity in the germ line has been found to depend on pi-RNAs, small repressive RNAs interacting with Piwi-family proteins and derived from larger transposable elements (TE)-derived primary transcripts. In particular, maternally transmitted I-element piRNAs originating from the 42AB region of polytene chromosomes were found to be involved in control of I element mobility. In the present work, we use a combination of cytological and molecular approaches to study the activity of I elements in three sublines of the inducer y; cn bw; sp isogenic strain and in dysgenic and non-dysgenic genetic backgrounds. Overall, the results of FISH and Southern blotting experiments clearly show that I elements are highly unstable in the Montpellier subline in the absence of classical dysgenic conditions. Such instability appears to be correlated to the amount of 5' and 3' I element transcripts detected by quantitative and real-time RT-PCR. The results of this study indicate that I elements can be highly active in the absence of a dysgenic crosses. Moreover, in the light of our results caution should be taken to assimilate the genomic annotation data on transposable elements to all y; cn bw sp sublines.
format	article
author	Roberta Moschetti Patrizio Dimitri Ruggiero Caizzi Nikolaj Junakovic
author_facet	Roberta Moschetti Patrizio Dimitri Ruggiero Caizzi Nikolaj Junakovic
author_sort	Roberta Moschetti
title	Genomic instability of I elements of Drosophila melanogaster in absence of dysgenic crosses.
title_short	Genomic instability of I elements of Drosophila melanogaster in absence of dysgenic crosses.
title_full	Genomic instability of I elements of Drosophila melanogaster in absence of dysgenic crosses.
title_fullStr	Genomic instability of I elements of Drosophila melanogaster in absence of dysgenic crosses.
title_full_unstemmed	Genomic instability of I elements of Drosophila melanogaster in absence of dysgenic crosses.
title_sort	genomic instability of i elements of drosophila melanogaster in absence of dysgenic crosses.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/8525f6a625a44519a0e006be8e8010c8
work_keys_str_mv	AT robertamoschetti genomicinstabilityofielementsofdrosophilamelanogasterinabsenceofdysgeniccrosses AT patriziodimitri genomicinstabilityofielementsofdrosophilamelanogasterinabsenceofdysgeniccrosses AT ruggierocaizzi genomicinstabilityofielementsofdrosophilamelanogasterinabsenceofdysgeniccrosses AT nikolajjunakovic genomicinstabilityofielementsofdrosophilamelanogasterinabsenceofdysgeniccrosses
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Genomic instability of I elements of Drosophila melanogaster in absence of dysgenic crosses.

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