The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in...

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Autores principales:	Hansjörg Götzke, Markus Kilisch, Markel Martínez-Carranza, Shama Sograte-Idrissi, Abirami Rajavel, Thomas Schlichthaerle, Niklas Engels, Ralf Jungmann, Pål Stenmark, Felipe Opazo, Steffen Frey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/852bbbdabda242768df8b322b02e6289
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spelling	oai:doaj.org-article:852bbbdabda242768df8b322b02e62892021-12-02T16:58:04ZThe ALFA-tag is a highly versatile tool for nanobody-based bioscience applications10.1038/s41467-019-12301-72041-1723https://doaj.org/article/852bbbdabda242768df8b322b02e62892019-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12301-7https://doaj.org/toc/2041-1723Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.Hansjörg GötzkeMarkus KilischMarkel Martínez-CarranzaShama Sograte-IdrissiAbirami RajavelThomas SchlichthaerleNiklas EngelsRalf JungmannPål StenmarkFelipe OpazoSteffen FreyNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Hansjörg Götzke Markus Kilisch Markel Martínez-Carranza Shama Sograte-Idrissi Abirami Rajavel Thomas Schlichthaerle Niklas Engels Ralf Jungmann Pål Stenmark Felipe Opazo Steffen Frey The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
description	Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.
format	article
author	Hansjörg Götzke Markus Kilisch Markel Martínez-Carranza Shama Sograte-Idrissi Abirami Rajavel Thomas Schlichthaerle Niklas Engels Ralf Jungmann Pål Stenmark Felipe Opazo Steffen Frey
author_facet	Hansjörg Götzke Markus Kilisch Markel Martínez-Carranza Shama Sograte-Idrissi Abirami Rajavel Thomas Schlichthaerle Niklas Engels Ralf Jungmann Pål Stenmark Felipe Opazo Steffen Frey
author_sort	Hansjörg Götzke
title	The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
title_short	The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
title_full	The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
title_fullStr	The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
title_full_unstemmed	The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
title_sort	alfa-tag is a highly versatile tool for nanobody-based bioscience applications
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/852bbbdabda242768df8b322b02e6289
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The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

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