Tobacco mosaic virus movement protein enhances the spread of RNA silencing.

Eukaryotic cells restrain the activity of foreign genetic elements, including viruses, through RNA silencing. Although viruses encode suppressors of silencing to support their propagation, viruses may also exploit silencing to regulate host gene expression or to control the level of their accumulati...

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Autores principales:	Hannes Vogler, Myoung-Ok Kwon, Vy Dang, Adrian Sambade, Monika Fasler, Jamie Ashby, Manfred Heinlein
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/852e733f4e0c4f46ada999e08fa9bbda
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spelling	oai:doaj.org-article:852e733f4e0c4f46ada999e08fa9bbda2021-11-25T05:46:38ZTobacco mosaic virus movement protein enhances the spread of RNA silencing.1553-73661553-737410.1371/journal.ppat.1000038https://doaj.org/article/852e733f4e0c4f46ada999e08fa9bbda2008-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18389061/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Eukaryotic cells restrain the activity of foreign genetic elements, including viruses, through RNA silencing. Although viruses encode suppressors of silencing to support their propagation, viruses may also exploit silencing to regulate host gene expression or to control the level of their accumulation and thus to reduce damage to the host. RNA silencing in plants propagates from cell to cell and systemically via a sequence-specific signal. Since the signal spreads between cells through plasmodesmata like the viruses themselves, virus-encoded plasmodesmata-manipulating movement proteins (MP) may have a central role in compatible virus:host interactions by suppressing or enhancing the spread of the signal. Here, we have addressed the propagation of GFP silencing in the presence and absence of MP and MP mutants. We show that the protein enhances the spread of silencing. Small RNA analysis indicates that MP does not enhance the silencing pathway but rather enhances the transport of the signal through plasmodesmata. The ability to enhance the spread of silencing is maintained by certain MP mutants that can move between cells but which have defects in subcellular localization and do not support the spread of viral RNA. Using MP expressing and non-expressing virus mutants with a disabled silencing suppressing function, we provide evidence indicating that viral MP contributes to anti-viral silencing during infection. Our results suggest a role of MP in controlling virus propagation in the infected host by supporting the spread of silencing signal. This activity of MP involves only a subset of its properties implicated in the spread of viral RNA.Hannes VoglerMyoung-Ok KwonVy DangAdrian SambadeMonika FaslerJamie AshbyManfred HeinleinPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 4, Iss 4, p e1000038 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Hannes Vogler Myoung-Ok Kwon Vy Dang Adrian Sambade Monika Fasler Jamie Ashby Manfred Heinlein Tobacco mosaic virus movement protein enhances the spread of RNA silencing.
description	Eukaryotic cells restrain the activity of foreign genetic elements, including viruses, through RNA silencing. Although viruses encode suppressors of silencing to support their propagation, viruses may also exploit silencing to regulate host gene expression or to control the level of their accumulation and thus to reduce damage to the host. RNA silencing in plants propagates from cell to cell and systemically via a sequence-specific signal. Since the signal spreads between cells through plasmodesmata like the viruses themselves, virus-encoded plasmodesmata-manipulating movement proteins (MP) may have a central role in compatible virus:host interactions by suppressing or enhancing the spread of the signal. Here, we have addressed the propagation of GFP silencing in the presence and absence of MP and MP mutants. We show that the protein enhances the spread of silencing. Small RNA analysis indicates that MP does not enhance the silencing pathway but rather enhances the transport of the signal through plasmodesmata. The ability to enhance the spread of silencing is maintained by certain MP mutants that can move between cells but which have defects in subcellular localization and do not support the spread of viral RNA. Using MP expressing and non-expressing virus mutants with a disabled silencing suppressing function, we provide evidence indicating that viral MP contributes to anti-viral silencing during infection. Our results suggest a role of MP in controlling virus propagation in the infected host by supporting the spread of silencing signal. This activity of MP involves only a subset of its properties implicated in the spread of viral RNA.
format	article
author	Hannes Vogler Myoung-Ok Kwon Vy Dang Adrian Sambade Monika Fasler Jamie Ashby Manfred Heinlein
author_facet	Hannes Vogler Myoung-Ok Kwon Vy Dang Adrian Sambade Monika Fasler Jamie Ashby Manfred Heinlein
author_sort	Hannes Vogler
title	Tobacco mosaic virus movement protein enhances the spread of RNA silencing.
title_short	Tobacco mosaic virus movement protein enhances the spread of RNA silencing.
title_full	Tobacco mosaic virus movement protein enhances the spread of RNA silencing.
title_fullStr	Tobacco mosaic virus movement protein enhances the spread of RNA silencing.
title_full_unstemmed	Tobacco mosaic virus movement protein enhances the spread of RNA silencing.
title_sort	tobacco mosaic virus movement protein enhances the spread of rna silencing.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/852e733f4e0c4f46ada999e08fa9bbda
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Tobacco mosaic virus movement protein enhances the spread of RNA silencing.

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