Ability of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls

The objective of the present study was to investigate whether fertility differences in bulls are reflected in variations of sperm quality when analysing only one ejaculate per male. Two experiments were performed. In the first experiment, frozen semen samples from 20 adult bulls were tested; 10 bull...

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Autores principales:	Jesús L. Yániz, Inmaculada Palacín, Miguel A. Silvestre, Carlos Olegario Hidalgo, Carolina Tamargo, Pilar Santolaria
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Bos taurus</i> spermiogram male fertility capacitation acrosome reaction Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/853a369637da40f2a7d94d21b53a64f3
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spelling	oai:doaj.org-article:853a369637da40f2a7d94d21b53a64f32021-11-25T16:47:21ZAbility of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls10.3390/biology101111352079-7737https://doaj.org/article/853a369637da40f2a7d94d21b53a64f32021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1135https://doaj.org/toc/2079-7737The objective of the present study was to investigate whether fertility differences in bulls are reflected in variations of sperm quality when analysing only one ejaculate per male. Two experiments were performed. In the first experiment, frozen semen samples from 20 adult bulls were tested; 10 bulls had high field fertility and 10 bulls had low field fertility. Analyses of sperm motility, membrane integrity, and membrane–acrosome integrity with the ISAS3Fun method were performed. Sperm morphometry of the fluorescence sperm subpopulations obtained with the ISAS3Fun method was also analysed. Significant differences between high- and low-fertility groups were only found with the ISAS3Fun technique, specifically in sperm acrosome integrity, the proportion of spermatozoa with an intact acrosome and damaged membrane, and in sperm head width of spermatozoa with intact structures. Discriminant analyses allowed us to correctly classify 90% of sperm samples in their fertility group using sperm quality parameters. Given that only the results obtained with the ISAS3Fun technique were related to bull fertility, we performed a second experiment aimed to validate the efficacy of this technique to detect the acrosomal integrity of bull spermatozoa, comparing them with the conventional FITC-PNA/propidium iodide (PNA/PI) combination under capacitating conditions. The results indicated that the ISAS3Fun combination provided an accurate assessment of both viability and acrosomal integrity for ejaculated spermatozoa, while the PNA/PI combination underestimated the extension of acrosomal damage due to false negatives. It was concluded that the simultaneous assessment of sperm plasma membranes and acrosome integrity with the ISAS3Fun method is precise and seems to have a greater potential to discriminate between high- and low-fertility bulls than more conventional in vitro sperm quality tests.Jesús L. YánizInmaculada PalacínMiguel A. SilvestreCarlos Olegario HidalgoCarolina TamargoPilar SantolariaMDPI AGarticle<i>Bos taurus</i>spermiogrammale fertilitycapacitationacrosome reactionBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1135, p 1135 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Bos taurus</i> spermiogram male fertility capacitation acrosome reaction Biology (General) QH301-705.5
spellingShingle	<i>Bos taurus</i> spermiogram male fertility capacitation acrosome reaction Biology (General) QH301-705.5 Jesús L. Yániz Inmaculada Palacín Miguel A. Silvestre Carlos Olegario Hidalgo Carolina Tamargo Pilar Santolaria Ability of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls
description	The objective of the present study was to investigate whether fertility differences in bulls are reflected in variations of sperm quality when analysing only one ejaculate per male. Two experiments were performed. In the first experiment, frozen semen samples from 20 adult bulls were tested; 10 bulls had high field fertility and 10 bulls had low field fertility. Analyses of sperm motility, membrane integrity, and membrane–acrosome integrity with the ISAS3Fun method were performed. Sperm morphometry of the fluorescence sperm subpopulations obtained with the ISAS3Fun method was also analysed. Significant differences between high- and low-fertility groups were only found with the ISAS3Fun technique, specifically in sperm acrosome integrity, the proportion of spermatozoa with an intact acrosome and damaged membrane, and in sperm head width of spermatozoa with intact structures. Discriminant analyses allowed us to correctly classify 90% of sperm samples in their fertility group using sperm quality parameters. Given that only the results obtained with the ISAS3Fun technique were related to bull fertility, we performed a second experiment aimed to validate the efficacy of this technique to detect the acrosomal integrity of bull spermatozoa, comparing them with the conventional FITC-PNA/propidium iodide (PNA/PI) combination under capacitating conditions. The results indicated that the ISAS3Fun combination provided an accurate assessment of both viability and acrosomal integrity for ejaculated spermatozoa, while the PNA/PI combination underestimated the extension of acrosomal damage due to false negatives. It was concluded that the simultaneous assessment of sperm plasma membranes and acrosome integrity with the ISAS3Fun method is precise and seems to have a greater potential to discriminate between high- and low-fertility bulls than more conventional in vitro sperm quality tests.
format	article
author	Jesús L. Yániz Inmaculada Palacín Miguel A. Silvestre Carlos Olegario Hidalgo Carolina Tamargo Pilar Santolaria
author_facet	Jesús L. Yániz Inmaculada Palacín Miguel A. Silvestre Carlos Olegario Hidalgo Carolina Tamargo Pilar Santolaria
author_sort	Jesús L. Yániz
title	Ability of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls
title_short	Ability of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls
title_full	Ability of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls
title_fullStr	Ability of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls
title_full_unstemmed	Ability of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls
title_sort	ability of the isas3fun method to detect sperm acrosome integrity and its potential to discriminate between high and low field fertility bulls
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/853a369637da40f2a7d94d21b53a64f3
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Ability of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls

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