Pre-injection of Zebrafish (Danio rerio) tnfb Polyclonal Antibody Decreases the Mortality of Vibrio vulnificus Infected Zebrafish

Tumor necrosis factor (TNF) plays an important role in an inflammatory cytokine storm. Over-secretion of TNF by the host in response to infection aggravates the disease. TNF expression level is positively correlated with the mortality caused by some bacterial infections. Therefore, using TNF antibod...

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Autores principales:	Suyi Li, Cong Jiang, Hua Chen, Lijuan Zhang, Ling Ke, Xu Chen, Chentao Lin
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	tnfb polyclonal antibody Vibrio vulnificus immunity zebrafish Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/853b32e839f540cb8213e043ab504e6c
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spelling	oai:doaj.org-article:853b32e839f540cb8213e043ab504e6c2021-11-18T09:13:52ZPre-injection of Zebrafish (Danio rerio) tnfb Polyclonal Antibody Decreases the Mortality of Vibrio vulnificus Infected Zebrafish2297-176910.3389/fvets.2021.741242https://doaj.org/article/853b32e839f540cb8213e043ab504e6c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.741242/fullhttps://doaj.org/toc/2297-1769Tumor necrosis factor (TNF) plays an important role in an inflammatory cytokine storm. Over-secretion of TNF by the host in response to infection aggravates the disease. TNF expression level is positively correlated with the mortality caused by some bacterial infections. Therefore, using TNF antibody may alleviate the inflammation to resist bacterial infections. The function of fish TNF-b antibody in bacterial infection is still unclear. In this study, infection models of Vibrio vulnificus FJ03-X2 strain with high pathogenicity and strong virulence were established in zebrafish (Danio rerio) fibroblast cell line (ZF4 cells) and zebrafish. Zebrafish tnfb (Zetnf-b) gene was cloned and expressed by Escherichia coli BL21 (DE3), and Zetnf-b polyclonal antibody was prepared. Pre-injection of Zetnf-b polyclonal antibody and AG-126 before infecting with V. vulnificus could increase the survival rate of zebrafish by 36.6 and 46.7%, respectively. Pre-injection of Zetnf-b polyclonal antibody could effectively decrease the mortality of zebrafish infected by V. vulnificus. Thus, TNF polyclonal antibody therapy could be considered as an effective strategy to control V. vulnificus in fish.Suyi LiCong JiangHua ChenLijuan ZhangLing KeXu ChenChentao LinChentao LinFrontiers Media S.A.articletnfbpolyclonal antibodyVibrio vulnificusimmunityzebrafishVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	tnfb polyclonal antibody Vibrio vulnificus immunity zebrafish Veterinary medicine SF600-1100
spellingShingle	tnfb polyclonal antibody Vibrio vulnificus immunity zebrafish Veterinary medicine SF600-1100 Suyi Li Cong Jiang Hua Chen Lijuan Zhang Ling Ke Xu Chen Chentao Lin Chentao Lin Pre-injection of Zebrafish (Danio rerio) tnfb Polyclonal Antibody Decreases the Mortality of Vibrio vulnificus Infected Zebrafish
description	Tumor necrosis factor (TNF) plays an important role in an inflammatory cytokine storm. Over-secretion of TNF by the host in response to infection aggravates the disease. TNF expression level is positively correlated with the mortality caused by some bacterial infections. Therefore, using TNF antibody may alleviate the inflammation to resist bacterial infections. The function of fish TNF-b antibody in bacterial infection is still unclear. In this study, infection models of Vibrio vulnificus FJ03-X2 strain with high pathogenicity and strong virulence were established in zebrafish (Danio rerio) fibroblast cell line (ZF4 cells) and zebrafish. Zebrafish tnfb (Zetnf-b) gene was cloned and expressed by Escherichia coli BL21 (DE3), and Zetnf-b polyclonal antibody was prepared. Pre-injection of Zetnf-b polyclonal antibody and AG-126 before infecting with V. vulnificus could increase the survival rate of zebrafish by 36.6 and 46.7%, respectively. Pre-injection of Zetnf-b polyclonal antibody could effectively decrease the mortality of zebrafish infected by V. vulnificus. Thus, TNF polyclonal antibody therapy could be considered as an effective strategy to control V. vulnificus in fish.
format	article
author	Suyi Li Cong Jiang Hua Chen Lijuan Zhang Ling Ke Xu Chen Chentao Lin Chentao Lin
author_facet	Suyi Li Cong Jiang Hua Chen Lijuan Zhang Ling Ke Xu Chen Chentao Lin Chentao Lin
author_sort	Suyi Li
title	Pre-injection of Zebrafish (Danio rerio) tnfb Polyclonal Antibody Decreases the Mortality of Vibrio vulnificus Infected Zebrafish
title_short	Pre-injection of Zebrafish (Danio rerio) tnfb Polyclonal Antibody Decreases the Mortality of Vibrio vulnificus Infected Zebrafish
title_full	Pre-injection of Zebrafish (Danio rerio) tnfb Polyclonal Antibody Decreases the Mortality of Vibrio vulnificus Infected Zebrafish
title_fullStr	Pre-injection of Zebrafish (Danio rerio) tnfb Polyclonal Antibody Decreases the Mortality of Vibrio vulnificus Infected Zebrafish
title_full_unstemmed	Pre-injection of Zebrafish (Danio rerio) tnfb Polyclonal Antibody Decreases the Mortality of Vibrio vulnificus Infected Zebrafish
title_sort	pre-injection of zebrafish (danio rerio) tnfb polyclonal antibody decreases the mortality of vibrio vulnificus infected zebrafish
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/853b32e839f540cb8213e043ab504e6c
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Pre-injection of Zebrafish (Danio rerio) tnfb Polyclonal Antibody Decreases the Mortality of Vibrio vulnificus Infected Zebrafish

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