Decreased Staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model

Mary C Machado,1 Keiko M Tarquinio,2 Thomas J Webster31School of Engineering, Brown University, Providence, RI; 2Division of Pediatric Critical Care Medicine, Rhode Island Hospital, Providence, RI; 3School of Engineering and Department of Orthopedics, Brown University, Providence, RI, USAAbstract: V...

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Autores principales:	Machado MC, Tarquinio KM, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/853b6820ff86418ab958ea6327b611cf
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spelling	oai:doaj.org-article:853b6820ff86418ab958ea6327b611cf2021-12-02T01:07:52ZDecreased Staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model1176-91141178-2013https://doaj.org/article/853b6820ff86418ab958ea6327b611cf2012-07-01T00:00:00Zhttp://www.dovepress.com/decreased-staphylococcus-aureus-biofilm-formation-on-nanomodified-endo-a10465https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Mary C Machado,1 Keiko M Tarquinio,2 Thomas J Webster31School of Engineering, Brown University, Providence, RI; 2Division of Pediatric Critical Care Medicine, Rhode Island Hospital, Providence, RI; 3School of Engineering and Department of Orthopedics, Brown University, Providence, RI, USAAbstract: Ventilator-associated pneumonia (VAP) is a serious and costly clinical problem. Specifically, receiving mechanical ventilation for over 24 hours increases the risk of VAP and is associated with high morbidity, mortality, and medical costs. Cost-effective endotracheal tubes (ETTs) that are resistant to bacterial infections could help prevent this problem. The objective of this study was to determine differences in the growth of Staphylococcus aureus on nanomodified and unmodified polyvinyl chloride (PVC) ETTs under dynamic airway conditions simulating a ventilated patient. PVC ETTs were modified to have nanometer surface features by soaking them in Rhizopus arrhisus, a fungal lipase. Twenty-four-hour experiments (supported by computational models) showed that airflow conditions within the ETT influenced both the location and the concentration of bacterial growth on the ETTs, especially within areas of tube curvature. More importantly, experiments revealed a 1.5 log reduction in the total number of S. aureus on the novel nanomodified ETTs compared with the conventional ETTs after 24 hours of airflow. This dynamic study showed that lipase etching can create nanorough surface features on PVC ETTs that suppress S. aureus growth, and thus may provide clinicians with an effective and inexpensive tool to combat VAP.Keywords: biofilm, laminar flow, ventilator-associated pneumonia, nanotechnology, endotracheal tubes, S. aureusMachado MCTarquinio KMWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3741-3750 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Machado MC Tarquinio KM Webster TJ Decreased Staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model
description	Mary C Machado,1 Keiko M Tarquinio,2 Thomas J Webster31School of Engineering, Brown University, Providence, RI; 2Division of Pediatric Critical Care Medicine, Rhode Island Hospital, Providence, RI; 3School of Engineering and Department of Orthopedics, Brown University, Providence, RI, USAAbstract: Ventilator-associated pneumonia (VAP) is a serious and costly clinical problem. Specifically, receiving mechanical ventilation for over 24 hours increases the risk of VAP and is associated with high morbidity, mortality, and medical costs. Cost-effective endotracheal tubes (ETTs) that are resistant to bacterial infections could help prevent this problem. The objective of this study was to determine differences in the growth of Staphylococcus aureus on nanomodified and unmodified polyvinyl chloride (PVC) ETTs under dynamic airway conditions simulating a ventilated patient. PVC ETTs were modified to have nanometer surface features by soaking them in Rhizopus arrhisus, a fungal lipase. Twenty-four-hour experiments (supported by computational models) showed that airflow conditions within the ETT influenced both the location and the concentration of bacterial growth on the ETTs, especially within areas of tube curvature. More importantly, experiments revealed a 1.5 log reduction in the total number of S. aureus on the novel nanomodified ETTs compared with the conventional ETTs after 24 hours of airflow. This dynamic study showed that lipase etching can create nanorough surface features on PVC ETTs that suppress S. aureus growth, and thus may provide clinicians with an effective and inexpensive tool to combat VAP.Keywords: biofilm, laminar flow, ventilator-associated pneumonia, nanotechnology, endotracheal tubes, S. aureus
format	article
author	Machado MC Tarquinio KM Webster TJ
author_facet	Machado MC Tarquinio KM Webster TJ
author_sort	Machado MC
title	Decreased Staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model
title_short	Decreased Staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model
title_full	Decreased Staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model
title_fullStr	Decreased Staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model
title_full_unstemmed	Decreased Staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model
title_sort	decreased staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/853b6820ff86418ab958ea6327b611cf
work_keys_str_mv	AT machadomc decreasedstaphylococcusaureusbiofilmformationonnanomodifiedendotrachealtubesadynamicairwaymodel AT tarquiniokm decreasedstaphylococcusaureusbiofilmformationonnanomodifiedendotrachealtubesadynamicairwaymodel AT webstertj decreasedstaphylococcusaureusbiofilmformationonnanomodifiedendotrachealtubesadynamicairwaymodel
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Decreased Staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model

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