LINC00839/miR-144-3p/WTAP (WT1 Associated protein) axis is involved in regulating hepatocellular carcinoma progression

The present work aimed to explore LINC00839 expression level and its function in hepatocellular carcinoma (HCC), and identify the downstream molecular mechanisms. qRT-PCR (Real-Time Quantitative Reverse Transcription PCR) and western blot were employed to detect mRNA and protein levels. Functional i...

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Autores principales:	Xingqin Zhou, Yazhou Chang, Lirong Zhu, Chaoyan Shen, Jing Qian, Renan Chang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	linc00839 mir-144-3p wtap hepatocellular carcinoma Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/8543c3d5f79d46ef98bce352218b851c
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spelling	oai:doaj.org-article:8543c3d5f79d46ef98bce352218b851c2021-12-01T14:41:00ZLINC00839/miR-144-3p/WTAP (WT1 Associated protein) axis is involved in regulating hepatocellular carcinoma progression2165-59792165-598710.1080/21655979.2021.1990578https://doaj.org/article/8543c3d5f79d46ef98bce352218b851c2021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1990578https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987The present work aimed to explore LINC00839 expression level and its function in hepatocellular carcinoma (HCC), and identify the downstream molecular mechanisms. qRT-PCR (Real-Time Quantitative Reverse Transcription PCR) and western blot were employed to detect mRNA and protein levels. Functional investigations were performed by flow cytometric-based apoptosis assay, CCK8 (Cell Counting Kit-8) assay, clone formation assay, Transwell migration and invasion assay. Functional interactions between LINC00839 and miR-144-3p or miR-144-3p and WTAP were validated by dual luciferase reporter assay. siRNA (small interfering RNA) was used for LINC00839 silencing, and microRNA mimic or inhibitor were employed to modulate miR-144-3p activity. LINC00839 was upregulated in HCC cells and tissues. Silencing LINC00839 suppressed the proliferation, invasion, migration of HCC cells and induced apoptosis. Additionally, LINC00839 served as a sponge to negatively impact on miR-144-3p activity, which contributed to the high expression of WTAP (WT1 Associated Protein) and the malignant phenotype of HCC cells. Our study revealed an oncogenic role of LINC00839 in HCC, and identified miR-144-3p/WTAP axis as downstream effectors mediating the oncogenic function of LINC00839. LINC00839 might serve as a potential therapeutic target and prognostic marker for HCC.Xingqin ZhouYazhou ChangLirong ZhuChaoyan ShenJing QianRenan ChangTaylor & Francis Grouparticlelinc00839mir-144-3pwtaphepatocellular carcinomaBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 10849-10861 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	linc00839 mir-144-3p wtap hepatocellular carcinoma Biotechnology TP248.13-248.65
spellingShingle	linc00839 mir-144-3p wtap hepatocellular carcinoma Biotechnology TP248.13-248.65 Xingqin Zhou Yazhou Chang Lirong Zhu Chaoyan Shen Jing Qian Renan Chang LINC00839/miR-144-3p/WTAP (WT1 Associated protein) axis is involved in regulating hepatocellular carcinoma progression
description	The present work aimed to explore LINC00839 expression level and its function in hepatocellular carcinoma (HCC), and identify the downstream molecular mechanisms. qRT-PCR (Real-Time Quantitative Reverse Transcription PCR) and western blot were employed to detect mRNA and protein levels. Functional investigations were performed by flow cytometric-based apoptosis assay, CCK8 (Cell Counting Kit-8) assay, clone formation assay, Transwell migration and invasion assay. Functional interactions between LINC00839 and miR-144-3p or miR-144-3p and WTAP were validated by dual luciferase reporter assay. siRNA (small interfering RNA) was used for LINC00839 silencing, and microRNA mimic or inhibitor were employed to modulate miR-144-3p activity. LINC00839 was upregulated in HCC cells and tissues. Silencing LINC00839 suppressed the proliferation, invasion, migration of HCC cells and induced apoptosis. Additionally, LINC00839 served as a sponge to negatively impact on miR-144-3p activity, which contributed to the high expression of WTAP (WT1 Associated Protein) and the malignant phenotype of HCC cells. Our study revealed an oncogenic role of LINC00839 in HCC, and identified miR-144-3p/WTAP axis as downstream effectors mediating the oncogenic function of LINC00839. LINC00839 might serve as a potential therapeutic target and prognostic marker for HCC.
format	article
author	Xingqin Zhou Yazhou Chang Lirong Zhu Chaoyan Shen Jing Qian Renan Chang
author_facet	Xingqin Zhou Yazhou Chang Lirong Zhu Chaoyan Shen Jing Qian Renan Chang
author_sort	Xingqin Zhou
title	LINC00839/miR-144-3p/WTAP (WT1 Associated protein) axis is involved in regulating hepatocellular carcinoma progression
title_short	LINC00839/miR-144-3p/WTAP (WT1 Associated protein) axis is involved in regulating hepatocellular carcinoma progression
title_full	LINC00839/miR-144-3p/WTAP (WT1 Associated protein) axis is involved in regulating hepatocellular carcinoma progression
title_fullStr	LINC00839/miR-144-3p/WTAP (WT1 Associated protein) axis is involved in regulating hepatocellular carcinoma progression
title_full_unstemmed	LINC00839/miR-144-3p/WTAP (WT1 Associated protein) axis is involved in regulating hepatocellular carcinoma progression
title_sort	linc00839/mir-144-3p/wtap (wt1 associated protein) axis is involved in regulating hepatocellular carcinoma progression
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8543c3d5f79d46ef98bce352218b851c
work_keys_str_mv	AT xingqinzhou linc00839mir1443pwtapwt1associatedproteinaxisisinvolvedinregulatinghepatocellularcarcinomaprogression AT yazhouchang linc00839mir1443pwtapwt1associatedproteinaxisisinvolvedinregulatinghepatocellularcarcinomaprogression AT lirongzhu linc00839mir1443pwtapwt1associatedproteinaxisisinvolvedinregulatinghepatocellularcarcinomaprogression AT chaoyanshen linc00839mir1443pwtapwt1associatedproteinaxisisinvolvedinregulatinghepatocellularcarcinomaprogression AT jingqian linc00839mir1443pwtapwt1associatedproteinaxisisinvolvedinregulatinghepatocellularcarcinomaprogression AT renanchang linc00839mir1443pwtapwt1associatedproteinaxisisinvolvedinregulatinghepatocellularcarcinomaprogression
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LINC00839/miR-144-3p/WTAP (WT1 Associated protein) axis is involved in regulating hepatocellular carcinoma progression

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