Genome-wide identification and tissue-specific expression analysis of UDP-glycosyltransferases genes confirm their abundance in Cicer arietinum (Chickpea) genome.

UDP-glycosyltransferases (EC 2.4.1.x; UGTs) are enzymes coded by an important gene family of higher plants. They are involved in the modification of secondary metabolites, phytohormones, and xenobiotics by transfer of sugar moieties from an activated nucleotide molecule to a wide range of acceptors....

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Autores principales:	Ranu Sharma, Vimal Rawat, C G Suresh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/854d494046f4426aa283e2714b32868f
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spelling	oai:doaj.org-article:854d494046f4426aa283e2714b32868f2021-11-25T05:57:41ZGenome-wide identification and tissue-specific expression analysis of UDP-glycosyltransferases genes confirm their abundance in Cicer arietinum (Chickpea) genome.1932-620310.1371/journal.pone.0109715https://doaj.org/article/854d494046f4426aa283e2714b32868f2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109715https://doaj.org/toc/1932-6203UDP-glycosyltransferases (EC 2.4.1.x; UGTs) are enzymes coded by an important gene family of higher plants. They are involved in the modification of secondary metabolites, phytohormones, and xenobiotics by transfer of sugar moieties from an activated nucleotide molecule to a wide range of acceptors. This modification regulates various functions like detoxification of xenobiotics, hormone homeostasis, and biosynthesis of secondary metabolites. Here, we describe the identification of 96 UGT genes in Cicer arietinum (CaUGT) and report their tissue-specific differential expression based on publically available RNA-seq and expressed sequence tag data. This analysis has established medium to high expression of 84 CaUGTs and low expression of 12 CaUGTs. We identified several closely related orthologs of CaUGTs in other genomes and compared their exon-intron arrangement. An attempt was made to assign functional specificity to chickpea UGTs by comparing substrate binding sites with experimentally determined specificity. These findings will assist in precise selection of candidate genes for various applications and understanding functional genomics of chickpea.Ranu SharmaVimal RawatC G SureshPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109715 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ranu Sharma Vimal Rawat C G Suresh Genome-wide identification and tissue-specific expression analysis of UDP-glycosyltransferases genes confirm their abundance in Cicer arietinum (Chickpea) genome.
description	UDP-glycosyltransferases (EC 2.4.1.x; UGTs) are enzymes coded by an important gene family of higher plants. They are involved in the modification of secondary metabolites, phytohormones, and xenobiotics by transfer of sugar moieties from an activated nucleotide molecule to a wide range of acceptors. This modification regulates various functions like detoxification of xenobiotics, hormone homeostasis, and biosynthesis of secondary metabolites. Here, we describe the identification of 96 UGT genes in Cicer arietinum (CaUGT) and report their tissue-specific differential expression based on publically available RNA-seq and expressed sequence tag data. This analysis has established medium to high expression of 84 CaUGTs and low expression of 12 CaUGTs. We identified several closely related orthologs of CaUGTs in other genomes and compared their exon-intron arrangement. An attempt was made to assign functional specificity to chickpea UGTs by comparing substrate binding sites with experimentally determined specificity. These findings will assist in precise selection of candidate genes for various applications and understanding functional genomics of chickpea.
format	article
author	Ranu Sharma Vimal Rawat C G Suresh
author_facet	Ranu Sharma Vimal Rawat C G Suresh
author_sort	Ranu Sharma
title	Genome-wide identification and tissue-specific expression analysis of UDP-glycosyltransferases genes confirm their abundance in Cicer arietinum (Chickpea) genome.
title_short	Genome-wide identification and tissue-specific expression analysis of UDP-glycosyltransferases genes confirm their abundance in Cicer arietinum (Chickpea) genome.
title_full	Genome-wide identification and tissue-specific expression analysis of UDP-glycosyltransferases genes confirm their abundance in Cicer arietinum (Chickpea) genome.
title_fullStr	Genome-wide identification and tissue-specific expression analysis of UDP-glycosyltransferases genes confirm their abundance in Cicer arietinum (Chickpea) genome.
title_full_unstemmed	Genome-wide identification and tissue-specific expression analysis of UDP-glycosyltransferases genes confirm their abundance in Cicer arietinum (Chickpea) genome.
title_sort	genome-wide identification and tissue-specific expression analysis of udp-glycosyltransferases genes confirm their abundance in cicer arietinum (chickpea) genome.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/854d494046f4426aa283e2714b32868f
work_keys_str_mv	AT ranusharma genomewideidentificationandtissuespecificexpressionanalysisofudpglycosyltransferasesgenesconfirmtheirabundanceincicerarietinumchickpeagenome AT vimalrawat genomewideidentificationandtissuespecificexpressionanalysisofudpglycosyltransferasesgenesconfirmtheirabundanceincicerarietinumchickpeagenome AT cgsuresh genomewideidentificationandtissuespecificexpressionanalysisofudpglycosyltransferasesgenesconfirmtheirabundanceincicerarietinumchickpeagenome
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Genome-wide identification and tissue-specific expression analysis of UDP-glycosyltransferases genes confirm their abundance in Cicer arietinum (Chickpea) genome.

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