Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes

Abstract High-throughput nucleic acid sequencing has greatly accelerated the discovery of viruses in the environment. Mosquitoes, because of their public health importance, are among those organisms whose viromes are being intensively characterized. Despite the deluge of sequence information, our un...

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Autores principales:	Panpim Thongsripong, James Angus Chandler, Pattamaporn Kittayapong, Bruce A. Wilcox, Durrell D. Kapan, Shannon N. Bennett
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85502251e4654e4587b91f4868f7c76a
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spelling	oai:doaj.org-article:85502251e4654e4587b91f4868f7c76a2021-12-02T18:27:48ZMetagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes10.1038/s41598-021-87122-02045-2322https://doaj.org/article/85502251e4654e4587b91f4868f7c76a2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87122-0https://doaj.org/toc/2045-2322Abstract High-throughput nucleic acid sequencing has greatly accelerated the discovery of viruses in the environment. Mosquitoes, because of their public health importance, are among those organisms whose viromes are being intensively characterized. Despite the deluge of sequence information, our understanding of the major drivers influencing the ecology of mosquito viromes remains limited. Using methods to increase the relative proportion of microbial RNA coupled with RNA-seq we characterize RNA viruses and other symbionts of three mosquito species collected along a rural to urban habitat gradient in Thailand. The full factorial study design allows us to explicitly investigate the relative importance of host species and habitat in structuring viral communities. We found that the pattern of virus presence was defined primarily by host species rather than by geographic locations or habitats. Our result suggests that insect-associated viruses display relatively narrow host ranges but are capable of spreading through a mosquito population at the geographical scale of our study. We also detected various single-celled and multicellular microorganisms such as bacteria, alveolates, fungi, and nematodes. Our study emphasizes the importance of including ecological information in viromic studies in order to gain further insights into viral ecology in systems where host specificity is driving both viral ecology and evolution.Panpim ThongsripongJames Angus ChandlerPattamaporn KittayapongBruce A. WilcoxDurrell D. KapanShannon N. BennettNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Panpim Thongsripong James Angus Chandler Pattamaporn Kittayapong Bruce A. Wilcox Durrell D. Kapan Shannon N. Bennett Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes
description	Abstract High-throughput nucleic acid sequencing has greatly accelerated the discovery of viruses in the environment. Mosquitoes, because of their public health importance, are among those organisms whose viromes are being intensively characterized. Despite the deluge of sequence information, our understanding of the major drivers influencing the ecology of mosquito viromes remains limited. Using methods to increase the relative proportion of microbial RNA coupled with RNA-seq we characterize RNA viruses and other symbionts of three mosquito species collected along a rural to urban habitat gradient in Thailand. The full factorial study design allows us to explicitly investigate the relative importance of host species and habitat in structuring viral communities. We found that the pattern of virus presence was defined primarily by host species rather than by geographic locations or habitats. Our result suggests that insect-associated viruses display relatively narrow host ranges but are capable of spreading through a mosquito population at the geographical scale of our study. We also detected various single-celled and multicellular microorganisms such as bacteria, alveolates, fungi, and nematodes. Our study emphasizes the importance of including ecological information in viromic studies in order to gain further insights into viral ecology in systems where host specificity is driving both viral ecology and evolution.
format	article
author	Panpim Thongsripong James Angus Chandler Pattamaporn Kittayapong Bruce A. Wilcox Durrell D. Kapan Shannon N. Bennett
author_facet	Panpim Thongsripong James Angus Chandler Pattamaporn Kittayapong Bruce A. Wilcox Durrell D. Kapan Shannon N. Bennett
author_sort	Panpim Thongsripong
title	Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes
title_short	Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes
title_full	Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes
title_fullStr	Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes
title_full_unstemmed	Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes
title_sort	metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/85502251e4654e4587b91f4868f7c76a
work_keys_str_mv	AT panpimthongsripong metagenomicshotgunsequencingrevealshostspeciesasanimportantdriverofviromecompositioninmosquitoes AT jamesanguschandler metagenomicshotgunsequencingrevealshostspeciesasanimportantdriverofviromecompositioninmosquitoes AT pattamapornkittayapong metagenomicshotgunsequencingrevealshostspeciesasanimportantdriverofviromecompositioninmosquitoes AT bruceawilcox metagenomicshotgunsequencingrevealshostspeciesasanimportantdriverofviromecompositioninmosquitoes AT durrelldkapan metagenomicshotgunsequencingrevealshostspeciesasanimportantdriverofviromecompositioninmosquitoes AT shannonnbennett metagenomicshotgunsequencingrevealshostspeciesasanimportantdriverofviromecompositioninmosquitoes
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Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes

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