Molecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia

It focused on the antiviral immune regulation of biofilm-localized protein kinase Dbf2p-related kinase 1 (NDR1) in viral pneumonia. Mouse alveolar monocyte RAW264.7 was used as blank control, and viral pneumonia cell model was prepared by infecting cells with respiratory syncytial virus (RSV). NDR1...

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Autores principales:	Haifeng Wang, Lina Bu, Fang Shu, Yun Bai, Feixiao Xue, Shanshan Shi, Daqing Sun
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	ndr1 il17 respiratory syncytial virus raw264.7 inflammatory responses immune response Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/8553e2cc64e343dbb30cc840d5291a28
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spelling	oai:doaj.org-article:8553e2cc64e343dbb30cc840d5291a282021-11-04T15:51:54ZMolecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia2165-59792165-598710.1080/21655979.2021.1996316https://doaj.org/article/8553e2cc64e343dbb30cc840d5291a282021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1996316https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987It focused on the antiviral immune regulation of biofilm-localized protein kinase Dbf2p-related kinase 1 (NDR1) in viral pneumonia. Mouse alveolar monocyte RAW264.7 was used as blank control, and viral pneumonia cell model was prepared by infecting cells with respiratory syncytial virus (RSV). NDR1 overexpression vector and siRNA interference sequences were synthesized, and overexpression/silence NDR1 cell model was fabricated. 50ng/mL interleukin 17 (IL-17) was given to stimulate. Enzyme-linked immuno sorbent assay (ELISA), quantitative reverse transcription PCR (RT-qRCR), and Western blot were performed to detect cytokines and chemokines, mRNA of inflammatory factors, and signal molecule protein expression. Notably, RSV infection increased RSV-F mRNA in RAW264.7 cells and reduced NDR1 mRNA and protein. Secretion levels of IL-6, interferon β (IFN-β), chemokine (C-X-C motif) ligand 2 (CXCL2), and chemokine (C-C motif) ligand 2 (CCL20) increased in the model group versus blank control (P<0.05). IL-6, IFN-β, tumor necrosis factor α (TNF-α), and toll-like receptor 3 (TLR3) mRNA were up-regulated (P<0.05). Extracellular signal-regulated kinase (ERK1/2), p38 protein phosphorylation, human recombinant 1 (TBK1), and nuclear factor kappa-B (NF-κB) protein levels increased (P<0.05). After overexpression of NDR1, the secretion levels of cytokines and chemokines, inflammatory factors mRNA, and signal molecule protein increased significantly. After NDR1 was silenced, cytokines and chemokines, inflammatory factors mRNA, and signal molecule protein were not significantly different versus blank control group (P>0.05). In short, NDR1 regulated innate immune response to viral pneumonia induced by IL-17, which can be used as a new target for treatment of IL-17-induced inflammatory response and autoimmune diseases.Haifeng WangLina BuFang ShuYun BaiFeixiao XueShanshan ShiDaqing SunTaylor & Francis Grouparticlendr1il17respiratory syncytial virusraw264.7inflammatory responsesimmune responseBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ndr1 il17 respiratory syncytial virus raw264.7 inflammatory responses immune response Biotechnology TP248.13-248.65
spellingShingle	ndr1 il17 respiratory syncytial virus raw264.7 inflammatory responses immune response Biotechnology TP248.13-248.65 Haifeng Wang Lina Bu Fang Shu Yun Bai Feixiao Xue Shanshan Shi Daqing Sun Molecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia
description	It focused on the antiviral immune regulation of biofilm-localized protein kinase Dbf2p-related kinase 1 (NDR1) in viral pneumonia. Mouse alveolar monocyte RAW264.7 was used as blank control, and viral pneumonia cell model was prepared by infecting cells with respiratory syncytial virus (RSV). NDR1 overexpression vector and siRNA interference sequences were synthesized, and overexpression/silence NDR1 cell model was fabricated. 50ng/mL interleukin 17 (IL-17) was given to stimulate. Enzyme-linked immuno sorbent assay (ELISA), quantitative reverse transcription PCR (RT-qRCR), and Western blot were performed to detect cytokines and chemokines, mRNA of inflammatory factors, and signal molecule protein expression. Notably, RSV infection increased RSV-F mRNA in RAW264.7 cells and reduced NDR1 mRNA and protein. Secretion levels of IL-6, interferon β (IFN-β), chemokine (C-X-C motif) ligand 2 (CXCL2), and chemokine (C-C motif) ligand 2 (CCL20) increased in the model group versus blank control (P<0.05). IL-6, IFN-β, tumor necrosis factor α (TNF-α), and toll-like receptor 3 (TLR3) mRNA were up-regulated (P<0.05). Extracellular signal-regulated kinase (ERK1/2), p38 protein phosphorylation, human recombinant 1 (TBK1), and nuclear factor kappa-B (NF-κB) protein levels increased (P<0.05). After overexpression of NDR1, the secretion levels of cytokines and chemokines, inflammatory factors mRNA, and signal molecule protein increased significantly. After NDR1 was silenced, cytokines and chemokines, inflammatory factors mRNA, and signal molecule protein were not significantly different versus blank control group (P>0.05). In short, NDR1 regulated innate immune response to viral pneumonia induced by IL-17, which can be used as a new target for treatment of IL-17-induced inflammatory response and autoimmune diseases.
format	article
author	Haifeng Wang Lina Bu Fang Shu Yun Bai Feixiao Xue Shanshan Shi Daqing Sun
author_facet	Haifeng Wang Lina Bu Fang Shu Yun Bai Feixiao Xue Shanshan Shi Daqing Sun
author_sort	Haifeng Wang
title	Molecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia
title_short	Molecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia
title_full	Molecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia
title_fullStr	Molecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia
title_full_unstemmed	Molecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia
title_sort	molecular mechanism of biofilm locator protein kinase dbf2p-related kinase 1 in regulating innate immune response to interleukin 17-induced viral pneumonia
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8553e2cc64e343dbb30cc840d5291a28
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Molecular Mechanism of Biofilm Locator Protein Kinase Dbf2p-related kinase 1 in Regulating Innate Immune Response to Interleukin 17-induced Viral Pneumonia

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