Magnetic nanoparticles from Magnetospirillum gryphiswaldense increase the efficacy of thermotherapy in a model of colon carcinoma.

Magnetic nanoparticles (MNPs) are capable of generate heating power under the influence of alternating magnetic fields (AMF); this behaviour recently opened new scenarios for advanced biomedical applications, mainly as new promising tumor therapies. In this paper we have tested magnetic nanoparticle...

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Autores principales: Silvia Mannucci, Leonardo Ghin, Giamaica Conti, Stefano Tambalo, Alessandro Lascialfari, Tomas Orlando, Donatella Benati, Paolo Bernardi, Nico Betterle, Roberto Bassi, Pasquina Marzola, Andrea Sbarbati
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spelling oai:doaj.org-article:856b073c1634465ba4c5866a07b39ca42021-11-25T05:57:45ZMagnetic nanoparticles from Magnetospirillum gryphiswaldense increase the efficacy of thermotherapy in a model of colon carcinoma.1932-620310.1371/journal.pone.0108959https://doaj.org/article/856b073c1634465ba4c5866a07b39ca42014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108959https://doaj.org/toc/1932-6203Magnetic nanoparticles (MNPs) are capable of generate heating power under the influence of alternating magnetic fields (AMF); this behaviour recently opened new scenarios for advanced biomedical applications, mainly as new promising tumor therapies. In this paper we have tested magnetic nanoparticles called magnetosomes (MNs): a class of MNPs naturally produced by magnetotactic bacteria. We extracted MNs from Magnetospirillum gryphiswaldense strain MSR-1 and tested the interaction with cellular elements and anti-neoplastic activity both in vitro and in vivo, with the aim of developing new therapeutic approaches for neoplastic diseases. In vitro experiments performed on Human Colon Carcinoma HT-29 cell cultures demonstrated a strong uptake of MNs with no evident signs of cytotoxicity and revealed three phases in the interaction: adherence, transport and accumulation in Golgi vesicles. In vivo studies were performed on subcutaneous tumors in mice; in this model MNs are administered by direct injection in the tumor volume, then a protocol consisting of three exposures to an AMF rated at 187 kHz and 23kA/m is carried out on alternate days, over a week. Tumors were monitored by Magnetic Resonance Imaging (MRI) to obtain information about MNs distribution and possible tissue modifications induced by hyperthermia. Histological analysis showed fibrous and necrotic areas close to MNs injection sites in mice subjected to a complete thermotherapy protocol. These results, although concerning a specific tumor model, could be useful to further investigate the feasibility and efficacy of protocols based on MFH. Magnetic nanoparticles naturally produced and extracted from bacteria seem to be promising candidates for theranostic applications in cancer therapy.Silvia MannucciLeonardo GhinGiamaica ContiStefano TambaloAlessandro LascialfariTomas OrlandoDonatella BenatiPaolo BernardiNico BetterleRoberto BassiPasquina MarzolaAndrea SbarbatiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e108959 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Silvia Mannucci
Leonardo Ghin
Giamaica Conti
Stefano Tambalo
Alessandro Lascialfari
Tomas Orlando
Donatella Benati
Paolo Bernardi
Nico Betterle
Roberto Bassi
Pasquina Marzola
Andrea Sbarbati
Magnetic nanoparticles from Magnetospirillum gryphiswaldense increase the efficacy of thermotherapy in a model of colon carcinoma.
description Magnetic nanoparticles (MNPs) are capable of generate heating power under the influence of alternating magnetic fields (AMF); this behaviour recently opened new scenarios for advanced biomedical applications, mainly as new promising tumor therapies. In this paper we have tested magnetic nanoparticles called magnetosomes (MNs): a class of MNPs naturally produced by magnetotactic bacteria. We extracted MNs from Magnetospirillum gryphiswaldense strain MSR-1 and tested the interaction with cellular elements and anti-neoplastic activity both in vitro and in vivo, with the aim of developing new therapeutic approaches for neoplastic diseases. In vitro experiments performed on Human Colon Carcinoma HT-29 cell cultures demonstrated a strong uptake of MNs with no evident signs of cytotoxicity and revealed three phases in the interaction: adherence, transport and accumulation in Golgi vesicles. In vivo studies were performed on subcutaneous tumors in mice; in this model MNs are administered by direct injection in the tumor volume, then a protocol consisting of three exposures to an AMF rated at 187 kHz and 23kA/m is carried out on alternate days, over a week. Tumors were monitored by Magnetic Resonance Imaging (MRI) to obtain information about MNs distribution and possible tissue modifications induced by hyperthermia. Histological analysis showed fibrous and necrotic areas close to MNs injection sites in mice subjected to a complete thermotherapy protocol. These results, although concerning a specific tumor model, could be useful to further investigate the feasibility and efficacy of protocols based on MFH. Magnetic nanoparticles naturally produced and extracted from bacteria seem to be promising candidates for theranostic applications in cancer therapy.
format article
author Silvia Mannucci
Leonardo Ghin
Giamaica Conti
Stefano Tambalo
Alessandro Lascialfari
Tomas Orlando
Donatella Benati
Paolo Bernardi
Nico Betterle
Roberto Bassi
Pasquina Marzola
Andrea Sbarbati
author_facet Silvia Mannucci
Leonardo Ghin
Giamaica Conti
Stefano Tambalo
Alessandro Lascialfari
Tomas Orlando
Donatella Benati
Paolo Bernardi
Nico Betterle
Roberto Bassi
Pasquina Marzola
Andrea Sbarbati
author_sort Silvia Mannucci
title Magnetic nanoparticles from Magnetospirillum gryphiswaldense increase the efficacy of thermotherapy in a model of colon carcinoma.
title_short Magnetic nanoparticles from Magnetospirillum gryphiswaldense increase the efficacy of thermotherapy in a model of colon carcinoma.
title_full Magnetic nanoparticles from Magnetospirillum gryphiswaldense increase the efficacy of thermotherapy in a model of colon carcinoma.
title_fullStr Magnetic nanoparticles from Magnetospirillum gryphiswaldense increase the efficacy of thermotherapy in a model of colon carcinoma.
title_full_unstemmed Magnetic nanoparticles from Magnetospirillum gryphiswaldense increase the efficacy of thermotherapy in a model of colon carcinoma.
title_sort magnetic nanoparticles from magnetospirillum gryphiswaldense increase the efficacy of thermotherapy in a model of colon carcinoma.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/856b073c1634465ba4c5866a07b39ca4
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