Real-time analysis and visualization for single-molecule based super-resolution microscopy.

Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy. We demonstrate a functional method for real-time reconstruction with automatic feedback control, without compromising the localization accuracy. C...

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Autores principales:	Adel Kechkar, Deepak Nair, Mike Heilemann, Daniel Choquet, Jean-Baptiste Sibarita
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/856c9f6c559e4c109817076017f1043c
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spelling	oai:doaj.org-article:856c9f6c559e4c109817076017f1043c2021-11-18T07:47:21ZReal-time analysis and visualization for single-molecule based super-resolution microscopy.1932-620310.1371/journal.pone.0062918https://doaj.org/article/856c9f6c559e4c109817076017f1043c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23646160/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy. We demonstrate a functional method for real-time reconstruction with automatic feedback control, without compromising the localization accuracy. Compatible with high frame rates of EM-CCD cameras, it relies on a wavelet segmentation algorithm, together with a mix of CPU/GPU implementation. A combination with Gaussian fitting allows direct access to 3D localization. Automatic feedback control ensures optimal molecule density throughout the acquisition process. With this method, we significantly improve the efficiency and feasibility of localization-based super-resolution microscopy.Adel KechkarDeepak NairMike HeilemannDaniel ChoquetJean-Baptiste SibaritaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62918 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adel Kechkar Deepak Nair Mike Heilemann Daniel Choquet Jean-Baptiste Sibarita Real-time analysis and visualization for single-molecule based super-resolution microscopy.
description	Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy. We demonstrate a functional method for real-time reconstruction with automatic feedback control, without compromising the localization accuracy. Compatible with high frame rates of EM-CCD cameras, it relies on a wavelet segmentation algorithm, together with a mix of CPU/GPU implementation. A combination with Gaussian fitting allows direct access to 3D localization. Automatic feedback control ensures optimal molecule density throughout the acquisition process. With this method, we significantly improve the efficiency and feasibility of localization-based super-resolution microscopy.
format	article
author	Adel Kechkar Deepak Nair Mike Heilemann Daniel Choquet Jean-Baptiste Sibarita
author_facet	Adel Kechkar Deepak Nair Mike Heilemann Daniel Choquet Jean-Baptiste Sibarita
author_sort	Adel Kechkar
title	Real-time analysis and visualization for single-molecule based super-resolution microscopy.
title_short	Real-time analysis and visualization for single-molecule based super-resolution microscopy.
title_full	Real-time analysis and visualization for single-molecule based super-resolution microscopy.
title_fullStr	Real-time analysis and visualization for single-molecule based super-resolution microscopy.
title_full_unstemmed	Real-time analysis and visualization for single-molecule based super-resolution microscopy.
title_sort	real-time analysis and visualization for single-molecule based super-resolution microscopy.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/856c9f6c559e4c109817076017f1043c
work_keys_str_mv	AT adelkechkar realtimeanalysisandvisualizationforsinglemoleculebasedsuperresolutionmicroscopy AT deepaknair realtimeanalysisandvisualizationforsinglemoleculebasedsuperresolutionmicroscopy AT mikeheilemann realtimeanalysisandvisualizationforsinglemoleculebasedsuperresolutionmicroscopy AT danielchoquet realtimeanalysisandvisualizationforsinglemoleculebasedsuperresolutionmicroscopy AT jeanbaptistesibarita realtimeanalysisandvisualizationforsinglemoleculebasedsuperresolutionmicroscopy
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Real-time analysis and visualization for single-molecule based super-resolution microscopy.

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