SNP-based assessment of genetic purity and diversity in maize hybrid breeding.

Assessment of genetic purity of parental inbred lines and their resultant F1 hybrids is an essential quality control check in maize hybrid breeding, variety release and seed production. In this study, genetic purity, parent-offspring relationship and diversity among the inbred lines were assessed us...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chimwemwe Josia, Kingstone Mashingaidze, Assefa B Amelework, Aleck Kondwakwenda, Cousin Musvosvi, Julia Sibiya
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/856d61a5c02345c7a145ae7863e6b56e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:856d61a5c02345c7a145ae7863e6b56e
record_format	dspace
spelling	oai:doaj.org-article:856d61a5c02345c7a145ae7863e6b56e2021-12-02T20:15:19ZSNP-based assessment of genetic purity and diversity in maize hybrid breeding.1932-620310.1371/journal.pone.0249505https://doaj.org/article/856d61a5c02345c7a145ae7863e6b56e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0249505https://doaj.org/toc/1932-6203Assessment of genetic purity of parental inbred lines and their resultant F1 hybrids is an essential quality control check in maize hybrid breeding, variety release and seed production. In this study, genetic purity, parent-offspring relationship and diversity among the inbred lines were assessed using 92 single-nucleotide polymorphism (SNP) markers. A total of 188 maize genotypes, comprising of 26 inbred lines, four doubled haploid (DH) lines and 158 single-cross maize hybrids were investigated in this study using Kompetitive Allele Specific Polymerase Chain Reaction (KASP) genotyping assays. The bi-allelic data was analyzed for genetic purity and diversity parameters using GenAlex software. The SNP markers were highly polymorphic and 90% had polymorphic information content (PIC) values of > 0.3. Pairwise genetic distances among the lines ranged from 0.05 to 0.56, indicating a high level of dissimilarity among the inbred lines. A maximum genetic distance of (0.56) was observed between inbred lines CKDHL0089 and CML443 while the lowest (0.05) was between I-42 and I-40. The majority (67%) of the inbred lines studied were genetically pure with residual heterozygosity of <5%, while only 33% had heterozygosity levels of >5%. Inbred lines, which were not pure, require purification through further inbreeding. Cluster analysis partitioned the lines into three distinct genetic clusters with the potential to contribute new beneficial alleles to the maize breeding program. Out of the 68 hybrids (43%) that passed the parent-offspring test, seven hybrids namely; SCHP29, SCHP95, SCHP94, SCHP134, SCHP44, SCHP114 and SCHP126, were selected as potential candidates for further evaluation and release due to their outstanding yield performance.Chimwemwe JosiaKingstone MashingaidzeAssefa B AmeleworkAleck KondwakwendaCousin MusvosviJulia SibiyaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0249505 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chimwemwe Josia Kingstone Mashingaidze Assefa B Amelework Aleck Kondwakwenda Cousin Musvosvi Julia Sibiya SNP-based assessment of genetic purity and diversity in maize hybrid breeding.
description	Assessment of genetic purity of parental inbred lines and their resultant F1 hybrids is an essential quality control check in maize hybrid breeding, variety release and seed production. In this study, genetic purity, parent-offspring relationship and diversity among the inbred lines were assessed using 92 single-nucleotide polymorphism (SNP) markers. A total of 188 maize genotypes, comprising of 26 inbred lines, four doubled haploid (DH) lines and 158 single-cross maize hybrids were investigated in this study using Kompetitive Allele Specific Polymerase Chain Reaction (KASP) genotyping assays. The bi-allelic data was analyzed for genetic purity and diversity parameters using GenAlex software. The SNP markers were highly polymorphic and 90% had polymorphic information content (PIC) values of > 0.3. Pairwise genetic distances among the lines ranged from 0.05 to 0.56, indicating a high level of dissimilarity among the inbred lines. A maximum genetic distance of (0.56) was observed between inbred lines CKDHL0089 and CML443 while the lowest (0.05) was between I-42 and I-40. The majority (67%) of the inbred lines studied were genetically pure with residual heterozygosity of <5%, while only 33% had heterozygosity levels of >5%. Inbred lines, which were not pure, require purification through further inbreeding. Cluster analysis partitioned the lines into three distinct genetic clusters with the potential to contribute new beneficial alleles to the maize breeding program. Out of the 68 hybrids (43%) that passed the parent-offspring test, seven hybrids namely; SCHP29, SCHP95, SCHP94, SCHP134, SCHP44, SCHP114 and SCHP126, were selected as potential candidates for further evaluation and release due to their outstanding yield performance.
format	article
author	Chimwemwe Josia Kingstone Mashingaidze Assefa B Amelework Aleck Kondwakwenda Cousin Musvosvi Julia Sibiya
author_facet	Chimwemwe Josia Kingstone Mashingaidze Assefa B Amelework Aleck Kondwakwenda Cousin Musvosvi Julia Sibiya
author_sort	Chimwemwe Josia
title	SNP-based assessment of genetic purity and diversity in maize hybrid breeding.
title_short	SNP-based assessment of genetic purity and diversity in maize hybrid breeding.
title_full	SNP-based assessment of genetic purity and diversity in maize hybrid breeding.
title_fullStr	SNP-based assessment of genetic purity and diversity in maize hybrid breeding.
title_full_unstemmed	SNP-based assessment of genetic purity and diversity in maize hybrid breeding.
title_sort	snp-based assessment of genetic purity and diversity in maize hybrid breeding.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/856d61a5c02345c7a145ae7863e6b56e
work_keys_str_mv	AT chimwemwejosia snpbasedassessmentofgeneticpurityanddiversityinmaizehybridbreeding AT kingstonemashingaidze snpbasedassessmentofgeneticpurityanddiversityinmaizehybridbreeding AT assefabamelework snpbasedassessmentofgeneticpurityanddiversityinmaizehybridbreeding AT aleckkondwakwenda snpbasedassessmentofgeneticpurityanddiversityinmaizehybridbreeding AT cousinmusvosvi snpbasedassessmentofgeneticpurityanddiversityinmaizehybridbreeding AT juliasibiya snpbasedassessmentofgeneticpurityanddiversityinmaizehybridbreeding
_version_	1718374592439910400

SNP-based assessment of genetic purity and diversity in maize hybrid breeding.

Ejemplares similares