Finnish Campylobacter jejuni strains of multilocus sequence type ST-22 complex have two lineages with different characteristics.

<h4>Background</h4>Campylobacter jejuni is the major cause of human bacterial gastroenteritis worldwide, and in a minority of cases, post-infectious complications may occur. ST-22 complex (usually Penner serotype 19) strains have been overrepresented among patients with postinfectious co...

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Autores principales:	Joana Revez, Mirko Rossi, Patrik Ellström, Caroline de Haan, Hilpi Rautelin, Marja-Liisa Hänninen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/856dd1228c9742a4b07698ea63e3115d
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spelling	oai:doaj.org-article:856dd1228c9742a4b07698ea63e3115d2021-11-18T07:35:50ZFinnish Campylobacter jejuni strains of multilocus sequence type ST-22 complex have two lineages with different characteristics.1932-620310.1371/journal.pone.0026880https://doaj.org/article/856dd1228c9742a4b07698ea63e3115d2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22039552/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Campylobacter jejuni is the major cause of human bacterial gastroenteritis worldwide, and in a minority of cases, post-infectious complications may occur. ST-22 complex (usually Penner serotype 19) strains have been overrepresented among patients with postinfectious complications of campylobacteriosis. We here present a characterization of a collection of 27 Finnish C. jejuni strains of ST-22 complex, from humans (22 strains) and animal sources (five strains), with the aim of contributing to our knowledge of the pathogenesis of C. jejuni infections.<h4>Methodology/principal findings</h4>All strains were analyzed by pulsed-field gel electrophoresis (PFGE) genotyping, lipo-oligosaccharide (LOS) locus class, Y-glutamyl transpeptidase (GGT) activity, in vitro biofilm formation ability, invasion and adhesion in HeLa cells and induction of IL-8 production. ST-22 complex contained five STs (ST-22; ST-1947; ST-1966; ST-3892; ST-3996) which were homogeneous in having sialylated LOS class A(1) but on the other hand were distinguished into two major lineages according to the major STs (ST-22 and ST-1947) by different PFGE genotypes and certain other characteristics. All ST-22 strains had similar SmaI PFGE profiles, were GGT positive, and formed biofilms, except one strain, while ST-1947 strains were all GGT negative, did not form biofilm, had significantly higher motility than ST-22 (p<0.05) and had their SmaI PFGE profile. Invasion and adhesion as well as induction of IL-8 production on HeLa cells were strain-dependent characteristics.<h4>Conclusions/significance</h4>ST-22 complex strains, reveal potential for molecular mimicry in host interactions upon infection as they all express sialylated LOS class A(1). The two major STs, ST-22 and ST-1947 formed two homogeneous lineages, which differed from each other both phenotypically and genetically, suggesting that the strains may have evolved separately, perhaps by interacting with different spectra of hosts. Further studies are needed in order to understand if these two lineages are associated with different disease outcomes.Joana RevezMirko RossiPatrik EllströmCaroline de HaanHilpi RautelinMarja-Liisa HänninenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26880 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joana Revez Mirko Rossi Patrik Ellström Caroline de Haan Hilpi Rautelin Marja-Liisa Hänninen Finnish Campylobacter jejuni strains of multilocus sequence type ST-22 complex have two lineages with different characteristics.
description	<h4>Background</h4>Campylobacter jejuni is the major cause of human bacterial gastroenteritis worldwide, and in a minority of cases, post-infectious complications may occur. ST-22 complex (usually Penner serotype 19) strains have been overrepresented among patients with postinfectious complications of campylobacteriosis. We here present a characterization of a collection of 27 Finnish C. jejuni strains of ST-22 complex, from humans (22 strains) and animal sources (five strains), with the aim of contributing to our knowledge of the pathogenesis of C. jejuni infections.<h4>Methodology/principal findings</h4>All strains were analyzed by pulsed-field gel electrophoresis (PFGE) genotyping, lipo-oligosaccharide (LOS) locus class, Y-glutamyl transpeptidase (GGT) activity, in vitro biofilm formation ability, invasion and adhesion in HeLa cells and induction of IL-8 production. ST-22 complex contained five STs (ST-22; ST-1947; ST-1966; ST-3892; ST-3996) which were homogeneous in having sialylated LOS class A(1) but on the other hand were distinguished into two major lineages according to the major STs (ST-22 and ST-1947) by different PFGE genotypes and certain other characteristics. All ST-22 strains had similar SmaI PFGE profiles, were GGT positive, and formed biofilms, except one strain, while ST-1947 strains were all GGT negative, did not form biofilm, had significantly higher motility than ST-22 (p<0.05) and had their SmaI PFGE profile. Invasion and adhesion as well as induction of IL-8 production on HeLa cells were strain-dependent characteristics.<h4>Conclusions/significance</h4>ST-22 complex strains, reveal potential for molecular mimicry in host interactions upon infection as they all express sialylated LOS class A(1). The two major STs, ST-22 and ST-1947 formed two homogeneous lineages, which differed from each other both phenotypically and genetically, suggesting that the strains may have evolved separately, perhaps by interacting with different spectra of hosts. Further studies are needed in order to understand if these two lineages are associated with different disease outcomes.
format	article
author	Joana Revez Mirko Rossi Patrik Ellström Caroline de Haan Hilpi Rautelin Marja-Liisa Hänninen
author_facet	Joana Revez Mirko Rossi Patrik Ellström Caroline de Haan Hilpi Rautelin Marja-Liisa Hänninen
author_sort	Joana Revez
title	Finnish Campylobacter jejuni strains of multilocus sequence type ST-22 complex have two lineages with different characteristics.
title_short	Finnish Campylobacter jejuni strains of multilocus sequence type ST-22 complex have two lineages with different characteristics.
title_full	Finnish Campylobacter jejuni strains of multilocus sequence type ST-22 complex have two lineages with different characteristics.
title_fullStr	Finnish Campylobacter jejuni strains of multilocus sequence type ST-22 complex have two lineages with different characteristics.
title_full_unstemmed	Finnish Campylobacter jejuni strains of multilocus sequence type ST-22 complex have two lineages with different characteristics.
title_sort	finnish campylobacter jejuni strains of multilocus sequence type st-22 complex have two lineages with different characteristics.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/856dd1228c9742a4b07698ea63e3115d
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Finnish Campylobacter jejuni strains of multilocus sequence type ST-22 complex have two lineages with different characteristics.

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