Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures

Yersinia enterocolitica is considered one of the most prevalent pathogens transmitted through milk and milk products. Therefore, we aimed to detect the prevalence of these bacteria in cheese and ice cream and study the influence of refrigeration and freezing on its growth patterns. A total of 80 sam...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Salwa S.Thabet, Manal H.Thabet
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	cheese ice cream pcr virulence genes y. enterocolitica Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.154573 https://doaj.org/article/857111bf818d4cf7b3dd80c417010458
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:857111bf818d4cf7b3dd80c417010458
record_format	dspace
spelling	oai:doaj.org-article:857111bf818d4cf7b3dd80c4170104582021-12-02T14:26:18ZViability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatureshttps://dx.doi.org/10.21608/javs.2021.1545731687-40722090-3308https://doaj.org/article/857111bf818d4cf7b3dd80c4170104582021-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_154573.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Yersinia enterocolitica is considered one of the most prevalent pathogens transmitted through milk and milk products. Therefore, we aimed to detect the prevalence of these bacteria in cheese and ice cream and study the influence of refrigeration and freezing on its growth patterns. A total of 80 samples of cheese and ice cream were collected from Assiut city, Egypt (40 samples each). The collected samples were examined for the isolation of Y. enterocolitica by the classical culture method and improved by the PCR technique. The incidence of Y. enterocolitica was 17.5 and 25.0 % in the examined cheese and ice cream samples by culture method, respectively, its prevalence in the tested cheese and ice cream samples basing on PCR were 7.5 and 15% since 42.8 and 60.0% of the isolated Y. enterocolotica were confirmed positive. Bio- typing and serotyping of the isolated strains revealed that 8 out of the confirmed strains were pathogenic ; Y. enterocolitica serotype O: 3 was the most prevalent strain, and all of the pathogenic strains carried the virulent ail gene. Nearly similar growth patterns of Y. enterocolitica were recorded during storage of cheese at 4 ± 2C and 30 ± 2ºC and, a significant difference was observed in the 3rd week, the organism found to be survived for 18 weeks with a mean value of 7.4 ± 0.5 and 6.9 ± 0.16 log cfu/ g for cheese stored at 4 ± 2C and 30 ± 2C respectively. In the case of ice cream, there was a significant difference between the behavior of Y. enterocolitica during freezing storage at -6 ± 2C and -18± 2ºC in the 2nd week, the mean value for the organism count on the 16th week was 5.3 ± 0.26 and 5.6 ± 0.39 log cfu/ g, respectively.Salwa S.ThabetManal H.ThabetEgyptian Society for Animal Managementarticlecheeseice creampcrvirulence genesy. enterocoliticaZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 2, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cheese ice cream pcr virulence genes y. enterocolitica Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	cheese ice cream pcr virulence genes y. enterocolitica Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Salwa S.Thabet Manal H.Thabet Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures
description	Yersinia enterocolitica is considered one of the most prevalent pathogens transmitted through milk and milk products. Therefore, we aimed to detect the prevalence of these bacteria in cheese and ice cream and study the influence of refrigeration and freezing on its growth patterns. A total of 80 samples of cheese and ice cream were collected from Assiut city, Egypt (40 samples each). The collected samples were examined for the isolation of Y. enterocolitica by the classical culture method and improved by the PCR technique. The incidence of Y. enterocolitica was 17.5 and 25.0 % in the examined cheese and ice cream samples by culture method, respectively, its prevalence in the tested cheese and ice cream samples basing on PCR were 7.5 and 15% since 42.8 and 60.0% of the isolated Y. enterocolotica were confirmed positive. Bio- typing and serotyping of the isolated strains revealed that 8 out of the confirmed strains were pathogenic ; Y. enterocolitica serotype O: 3 was the most prevalent strain, and all of the pathogenic strains carried the virulent ail gene. Nearly similar growth patterns of Y. enterocolitica were recorded during storage of cheese at 4 ± 2C and 30 ± 2ºC and, a significant difference was observed in the 3rd week, the organism found to be survived for 18 weeks with a mean value of 7.4 ± 0.5 and 6.9 ± 0.16 log cfu/ g for cheese stored at 4 ± 2C and 30 ± 2C respectively. In the case of ice cream, there was a significant difference between the behavior of Y. enterocolitica during freezing storage at -6 ± 2C and -18± 2ºC in the 2nd week, the mean value for the organism count on the 16th week was 5.3 ± 0.26 and 5.6 ± 0.39 log cfu/ g, respectively.
format	article
author	Salwa S.Thabet Manal H.Thabet
author_facet	Salwa S.Thabet Manal H.Thabet
author_sort	Salwa S.Thabet
title	Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures
title_short	Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures
title_full	Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures
title_fullStr	Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures
title_full_unstemmed	Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures
title_sort	viability of the isolated yersinia enterocolitica strains from damietta cheese and ice cream at different refrigeration and freezing temperatures
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.154573 https://doaj.org/article/857111bf818d4cf7b3dd80c417010458
work_keys_str_mv	AT salwasthabet viabilityoftheisolatedyersiniaenterocoliticastrainsfromdamiettacheeseandicecreamatdifferentrefrigerationandfreezingtemperatures AT manalhthabet viabilityoftheisolatedyersiniaenterocoliticastrainsfromdamiettacheeseandicecreamatdifferentrefrigerationandfreezingtemperatures
_version_	1718391379009208320

Viability of The Isolated Yersinia enterocolitica Strains from Damietta Cheese and Ice Cream at Different Refrigeration and Freezing Temperatures

Ejemplares similares