Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy

Abstract Leprosy reactions are immune processes that cause neural damage in individuals with leprosy. As periodontitis is an infectious disease related to its development, specific antibodies to periodontal pathogens must be evaluated to better understand the humoral mechanisms underlying this relat...

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Autores principales:	Mariana Costa Calheira, Soraya Castro Trindade, Michelle Miranda Lopes Falcão, Luciana Sales Conceição Barbosa, Gislene Regina Batista Carvalho, Paulo Roberto Lima Machado, Isaac Suzart Gomes-Filho, Elisangela de Jesus Campos, Paulo Cirino de Carvalho-Filho, Márcia Tosta Xavier, Antonio Pedro Fróes de Farias, José Tadeu Raynal Rocha Filho, Johelle de Santana Passos-Soares
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Leprosy Periodontitis Saliva Immunoassay protocols Enzyme-linked immunosorbent assay P. gingivalis Biotechnology TP248.13-248.65 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/85711476866b4d71b73f5978c9051736
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spelling	oai:doaj.org-article:85711476866b4d71b73f5978c90517362021-11-21T12:09:05ZImmunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy10.1186/s13568-021-01312-72191-0855https://doaj.org/article/85711476866b4d71b73f5978c90517362021-11-01T00:00:00Zhttps://doi.org/10.1186/s13568-021-01312-7https://doaj.org/toc/2191-0855Abstract Leprosy reactions are immune processes that cause neural damage in individuals with leprosy. As periodontitis is an infectious disease related to its development, specific antibodies to periodontal pathogens must be evaluated to better understand the humoral mechanisms underlying this relationship. Therefore, the objective of this study was to standardize an immunoassay to measure IgA specific to P. gingivalis antigens in the saliva of individuals with leprosy. An ELISA checkerboard titration was performed. A validation test involving 53 individuals with leprosy, 24 with and 19 without periodontitis, was conducted and a ROC curve constructed to calculate sensitivity and specificity. The coefficient of the optical densities was 2.21 and 2.66 for P. gingivalis crude extract and the recombinant protein HmuY, respectively. Sensitivity and specificity for the P. gingivalis crude extract were 66.7% and 73.7%, respectively, and for HmuY, were 62.5% and 52.6%, respectively. Specific recognition of P. gingivalis occurred predominantly in individuals with periodontitis, which validates the use of this test for studying periodontitis in individuals with leprosy. Trial registration CAEE 64476117.3.0000.0049, 21/07/2017, retrospectively registeredMariana Costa CalheiraSoraya Castro TrindadeMichelle Miranda Lopes FalcãoLuciana Sales Conceição BarbosaGislene Regina Batista CarvalhoPaulo Roberto Lima MachadoIsaac Suzart Gomes-FilhoElisangela de Jesus CamposPaulo Cirino de Carvalho-FilhoMárcia Tosta XavierAntonio Pedro Fróes de FariasJosé Tadeu Raynal Rocha FilhoJohelle de Santana Passos-SoaresSpringerOpenarticleLeprosyPeriodontitisSalivaImmunoassay protocolsEnzyme-linked immunosorbent assayP. gingivalisBiotechnologyTP248.13-248.65MicrobiologyQR1-502ENAMB Express, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Leprosy Periodontitis Saliva Immunoassay protocols Enzyme-linked immunosorbent assay P. gingivalis Biotechnology TP248.13-248.65 Microbiology QR1-502
spellingShingle	Leprosy Periodontitis Saliva Immunoassay protocols Enzyme-linked immunosorbent assay P. gingivalis Biotechnology TP248.13-248.65 Microbiology QR1-502 Mariana Costa Calheira Soraya Castro Trindade Michelle Miranda Lopes Falcão Luciana Sales Conceição Barbosa Gislene Regina Batista Carvalho Paulo Roberto Lima Machado Isaac Suzart Gomes-Filho Elisangela de Jesus Campos Paulo Cirino de Carvalho-Filho Márcia Tosta Xavier Antonio Pedro Fróes de Farias José Tadeu Raynal Rocha Filho Johelle de Santana Passos-Soares Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy
description	Abstract Leprosy reactions are immune processes that cause neural damage in individuals with leprosy. As periodontitis is an infectious disease related to its development, specific antibodies to periodontal pathogens must be evaluated to better understand the humoral mechanisms underlying this relationship. Therefore, the objective of this study was to standardize an immunoassay to measure IgA specific to P. gingivalis antigens in the saliva of individuals with leprosy. An ELISA checkerboard titration was performed. A validation test involving 53 individuals with leprosy, 24 with and 19 without periodontitis, was conducted and a ROC curve constructed to calculate sensitivity and specificity. The coefficient of the optical densities was 2.21 and 2.66 for P. gingivalis crude extract and the recombinant protein HmuY, respectively. Sensitivity and specificity for the P. gingivalis crude extract were 66.7% and 73.7%, respectively, and for HmuY, were 62.5% and 52.6%, respectively. Specific recognition of P. gingivalis occurred predominantly in individuals with periodontitis, which validates the use of this test for studying periodontitis in individuals with leprosy. Trial registration CAEE 64476117.3.0000.0049, 21/07/2017, retrospectively registered
format	article
author	Mariana Costa Calheira Soraya Castro Trindade Michelle Miranda Lopes Falcão Luciana Sales Conceição Barbosa Gislene Regina Batista Carvalho Paulo Roberto Lima Machado Isaac Suzart Gomes-Filho Elisangela de Jesus Campos Paulo Cirino de Carvalho-Filho Márcia Tosta Xavier Antonio Pedro Fróes de Farias José Tadeu Raynal Rocha Filho Johelle de Santana Passos-Soares
author_facet	Mariana Costa Calheira Soraya Castro Trindade Michelle Miranda Lopes Falcão Luciana Sales Conceição Barbosa Gislene Regina Batista Carvalho Paulo Roberto Lima Machado Isaac Suzart Gomes-Filho Elisangela de Jesus Campos Paulo Cirino de Carvalho-Filho Márcia Tosta Xavier Antonio Pedro Fróes de Farias José Tadeu Raynal Rocha Filho Johelle de Santana Passos-Soares
author_sort	Mariana Costa Calheira
title	Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy
title_short	Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy
title_full	Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy
title_fullStr	Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy
title_full_unstemmed	Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy
title_sort	immunoassay standardization for the detection of immunoglobulin a (iga) against porphyromonas gingivalis antigens in saliva of individuals with and without leprosy
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/85711476866b4d71b73f5978c9051736
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Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy

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