The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin

Abstract XCR1 is selectively expressed on a conventional dendritic cell subset, the cDC1 subset, through phylogenetically distant species. The outcome of antigen-targeting to XCR1 may therefore be similar across species, permitting the translation of results from experimental models to human and vet...

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Autores principales:	Charlotte Deloizy, Even Fossum, Christophe Barnier-Quer, Céline Urien, Tiphany Chrun, Audrey Duval, Maelle Codjovi, Edwige Bouguyon, Pauline Maisonnasse, Pierre-Louis Hervé, Céline Barc, Olivier Boulesteix, Jérémy Pezant, Christophe Chevalier, Nicolas Collin, Marc Dalod, Bjarne Bogen, Nicolas Bertho, Isabelle Schwartz-Cornil
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8572466da2a7445f89ee0eacb55a322c
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spelling	oai:doaj.org-article:8572466da2a7445f89ee0eacb55a322c2021-12-02T15:05:41ZThe anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin10.1038/s41598-017-07372-92045-2322https://doaj.org/article/8572466da2a7445f89ee0eacb55a322c2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07372-9https://doaj.org/toc/2045-2322Abstract XCR1 is selectively expressed on a conventional dendritic cell subset, the cDC1 subset, through phylogenetically distant species. The outcome of antigen-targeting to XCR1 may therefore be similar across species, permitting the translation of results from experimental models to human and veterinary applications. Here we evaluated in pigs the immunogenicity of bivalent protein structures made of XCL1 fused to the external portion of the influenza virus M2 proton pump, which is conserved through strains and a candidate for universal influenza vaccines. Pigs represent a relevant target of such universal vaccines as pigs can be infected by swine, human and avian strains. We found that cDC1 were the only cell type labeled by XCR1-targeted mCherry upon intradermal injection in pig skin. XCR1-targeted M2e induced higher IgG responses in seronegative and seropositive pigs as compared to non-targeted M2e. The IgG response was less significantly enhanced by CpG than by XCR1 targeting, and CpG did not further increase the response elicited by XCR1 targeting. Monophosphoryl lipid A with neutral liposomes did not have significant effect. Thus altogether M2e-targeting to XCR1 shows promises for a trans-species universal influenza vaccine strategy, possibly avoiding the use of classical adjuvants.Charlotte DeloizyEven FossumChristophe Barnier-QuerCéline UrienTiphany ChrunAudrey DuvalMaelle CodjoviEdwige BouguyonPauline MaisonnassePierre-Louis HervéCéline BarcOlivier BoulesteixJérémy PezantChristophe ChevalierNicolas CollinMarc DalodBjarne BogenNicolas BerthoIsabelle Schwartz-CornilNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charlotte Deloizy Even Fossum Christophe Barnier-Quer Céline Urien Tiphany Chrun Audrey Duval Maelle Codjovi Edwige Bouguyon Pauline Maisonnasse Pierre-Louis Hervé Céline Barc Olivier Boulesteix Jérémy Pezant Christophe Chevalier Nicolas Collin Marc Dalod Bjarne Bogen Nicolas Bertho Isabelle Schwartz-Cornil The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin
description	Abstract XCR1 is selectively expressed on a conventional dendritic cell subset, the cDC1 subset, through phylogenetically distant species. The outcome of antigen-targeting to XCR1 may therefore be similar across species, permitting the translation of results from experimental models to human and veterinary applications. Here we evaluated in pigs the immunogenicity of bivalent protein structures made of XCL1 fused to the external portion of the influenza virus M2 proton pump, which is conserved through strains and a candidate for universal influenza vaccines. Pigs represent a relevant target of such universal vaccines as pigs can be infected by swine, human and avian strains. We found that cDC1 were the only cell type labeled by XCR1-targeted mCherry upon intradermal injection in pig skin. XCR1-targeted M2e induced higher IgG responses in seronegative and seropositive pigs as compared to non-targeted M2e. The IgG response was less significantly enhanced by CpG than by XCR1 targeting, and CpG did not further increase the response elicited by XCR1 targeting. Monophosphoryl lipid A with neutral liposomes did not have significant effect. Thus altogether M2e-targeting to XCR1 shows promises for a trans-species universal influenza vaccine strategy, possibly avoiding the use of classical adjuvants.
format	article
author	Charlotte Deloizy Even Fossum Christophe Barnier-Quer Céline Urien Tiphany Chrun Audrey Duval Maelle Codjovi Edwige Bouguyon Pauline Maisonnasse Pierre-Louis Hervé Céline Barc Olivier Boulesteix Jérémy Pezant Christophe Chevalier Nicolas Collin Marc Dalod Bjarne Bogen Nicolas Bertho Isabelle Schwartz-Cornil
author_facet	Charlotte Deloizy Even Fossum Christophe Barnier-Quer Céline Urien Tiphany Chrun Audrey Duval Maelle Codjovi Edwige Bouguyon Pauline Maisonnasse Pierre-Louis Hervé Céline Barc Olivier Boulesteix Jérémy Pezant Christophe Chevalier Nicolas Collin Marc Dalod Bjarne Bogen Nicolas Bertho Isabelle Schwartz-Cornil
author_sort	Charlotte Deloizy
title	The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin
title_short	The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin
title_full	The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin
title_fullStr	The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin
title_full_unstemmed	The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin
title_sort	anti-influenza m2e antibody response is promoted by xcr1 targeting in pig skin
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8572466da2a7445f89ee0eacb55a322c
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The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin

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