Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography

Arifiyanto D, Basyuni M, Sumardi, Putri LAP, Siregar ES, Risnasari I, Syahputra I. 2017. Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography. Biodiversitas 18: 1487- 1492. The problems that have been faced by...

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Autores principales: DENI ARIFIYANTO, MOHAMMAD BASYUNI, SUMARDI SUMARDI, LOLLIE AGUSTINA PANCAWARASWATI PUTRI, ETTI SARTINA SIREGAR, IWAN RISNASARI, INDRA SYAHPUTRA
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Publicado: MBI & UNS Solo 2017
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spelling oai:doaj.org-article:85763af1d03545a5b47fb10385b343fd2021-11-16T14:00:12ZShort Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography1412-033X2085-472210.13057/biodiv/d180426https://doaj.org/article/85763af1d03545a5b47fb10385b343fd2017-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2103https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Arifiyanto D, Basyuni M, Sumardi, Putri LAP, Siregar ES, Risnasari I, Syahputra I. 2017. Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography. Biodiversitas 18: 1487- 1492. The problems that have been faced by palm oil breeders are the length of time and high costs to discover the type of palm oil (Elaeis guineensis) fruit namely Dura, Pisifera, or Tenera, before using as a seed parent. These conditions are ineffective and add to the cost of maintenance and the production of seedling is more expensive. The present study describes the occurrence and cluster analysis of palm oil fruits using two-dimensional thin layer chromatography (2D-TLC). The leaves and fruit on each fruit mesocarp and shell, commercial seed on each fruit type were sampled through direct determination, plant nurseries, commercial seed and unknown type of palm oil. 2D-TLC chromatograms of hexane extracts showed diversity in palm oil fruits: Dura had ficaprenol-type polyprenol (C50-C60) and no carbon chain-length of polyprenol and dolichols (C85-C100) were found. In Tenera polyprenols of C45-C60 and C90-C100 occurred and dolichols of C85-C105 as well, where polyprenols of C45 and C105 and dolichol of C105 found in Tenera were not detected in Pisifera. To confirm these findings, cluster analysis was drawn using the UPGMA method. The dendrogram demonstrated that the three types of palm oil were grouped to fruit type, suggesting that the occurrence of polyisoprenoids in palm oil fruits were chemotaxonomically significant.DENI ARIFIYANTOMOHAMMAD BASYUNISUMARDI SUMARDILOLLIE AGUSTINA PANCAWARASWATI PUTRIETTI SARTINA SIREGARIWAN RISNASARIINDRA SYAHPUTRAMBI & UNS Soloarticlechemotaxonomic markerelaeis guineensisteneratwo-dimensional thin layer chromatographyBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 4, Pp 1487-1492 (2017)
institution DOAJ
collection DOAJ
language EN
topic chemotaxonomic marker
elaeis guineensis
tenera
two-dimensional thin layer chromatography
Biology (General)
QH301-705.5
spellingShingle chemotaxonomic marker
elaeis guineensis
tenera
two-dimensional thin layer chromatography
Biology (General)
QH301-705.5
DENI ARIFIYANTO
MOHAMMAD BASYUNI
SUMARDI SUMARDI
LOLLIE AGUSTINA PANCAWARASWATI PUTRI
ETTI SARTINA SIREGAR
IWAN RISNASARI
INDRA SYAHPUTRA
Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography
description Arifiyanto D, Basyuni M, Sumardi, Putri LAP, Siregar ES, Risnasari I, Syahputra I. 2017. Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography. Biodiversitas 18: 1487- 1492. The problems that have been faced by palm oil breeders are the length of time and high costs to discover the type of palm oil (Elaeis guineensis) fruit namely Dura, Pisifera, or Tenera, before using as a seed parent. These conditions are ineffective and add to the cost of maintenance and the production of seedling is more expensive. The present study describes the occurrence and cluster analysis of palm oil fruits using two-dimensional thin layer chromatography (2D-TLC). The leaves and fruit on each fruit mesocarp and shell, commercial seed on each fruit type were sampled through direct determination, plant nurseries, commercial seed and unknown type of palm oil. 2D-TLC chromatograms of hexane extracts showed diversity in palm oil fruits: Dura had ficaprenol-type polyprenol (C50-C60) and no carbon chain-length of polyprenol and dolichols (C85-C100) were found. In Tenera polyprenols of C45-C60 and C90-C100 occurred and dolichols of C85-C105 as well, where polyprenols of C45 and C105 and dolichol of C105 found in Tenera were not detected in Pisifera. To confirm these findings, cluster analysis was drawn using the UPGMA method. The dendrogram demonstrated that the three types of palm oil were grouped to fruit type, suggesting that the occurrence of polyisoprenoids in palm oil fruits were chemotaxonomically significant.
format article
author DENI ARIFIYANTO
MOHAMMAD BASYUNI
SUMARDI SUMARDI
LOLLIE AGUSTINA PANCAWARASWATI PUTRI
ETTI SARTINA SIREGAR
IWAN RISNASARI
INDRA SYAHPUTRA
author_facet DENI ARIFIYANTO
MOHAMMAD BASYUNI
SUMARDI SUMARDI
LOLLIE AGUSTINA PANCAWARASWATI PUTRI
ETTI SARTINA SIREGAR
IWAN RISNASARI
INDRA SYAHPUTRA
author_sort DENI ARIFIYANTO
title Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography
title_short Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography
title_full Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography
title_fullStr Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography
title_full_unstemmed Short Communication: Occurrence and cluster analysis of palm oil (Elaeis guineensis) fruit type using two-dimensional thin layer chromatography
title_sort short communication: occurrence and cluster analysis of palm oil (elaeis guineensis) fruit type using two-dimensional thin layer chromatography
publisher MBI & UNS Solo
publishDate 2017
url https://doaj.org/article/85763af1d03545a5b47fb10385b343fd
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