The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII

Abstract Photosynthesis is tightly regulated in order to withstand dynamic light environments. Under high light intensities, a mechanism known as non-photochemical quenching (NPQ) dissipates excess excitation energy, protecting the photosynthetic machinery from damage. An obstacle that lies in the w...

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Autores principales:	Lauren Nicol, Roberta Croce
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85784a06f2774c49ba2955845b7494c0
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spelling	oai:doaj.org-article:85784a06f2774c49ba2955845b7494c02021-12-02T18:17:42ZThe PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII10.1038/s41598-021-86975-92045-2322https://doaj.org/article/85784a06f2774c49ba2955845b7494c02021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86975-9https://doaj.org/toc/2045-2322Abstract Photosynthesis is tightly regulated in order to withstand dynamic light environments. Under high light intensities, a mechanism known as non-photochemical quenching (NPQ) dissipates excess excitation energy, protecting the photosynthetic machinery from damage. An obstacle that lies in the way of understanding the molecular mechanism of NPQ is the large gap between in vitro and in vivo studies. On the one hand, the complexity of the photosynthetic membrane makes it challenging to obtain molecular information from in vivo experiments. On the other hand, a suitable in vitro system for the study of quenching is not available. Here we have developed a minimal NPQ system using proteoliposomes. With this, we demonstrate that the combination of low pH and PsbS is both necessary and sufficient to induce quenching in LHCII, the main antenna complex of plants. This proteoliposome system can be further exploited to gain more insight into how PsbS and other factors (e.g. zeaxanthin) influence the quenching mechanism observed in LHCII.Lauren NicolRoberta CroceNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lauren Nicol Roberta Croce The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII
description	Abstract Photosynthesis is tightly regulated in order to withstand dynamic light environments. Under high light intensities, a mechanism known as non-photochemical quenching (NPQ) dissipates excess excitation energy, protecting the photosynthetic machinery from damage. An obstacle that lies in the way of understanding the molecular mechanism of NPQ is the large gap between in vitro and in vivo studies. On the one hand, the complexity of the photosynthetic membrane makes it challenging to obtain molecular information from in vivo experiments. On the other hand, a suitable in vitro system for the study of quenching is not available. Here we have developed a minimal NPQ system using proteoliposomes. With this, we demonstrate that the combination of low pH and PsbS is both necessary and sufficient to induce quenching in LHCII, the main antenna complex of plants. This proteoliposome system can be further exploited to gain more insight into how PsbS and other factors (e.g. zeaxanthin) influence the quenching mechanism observed in LHCII.
format	article
author	Lauren Nicol Roberta Croce
author_facet	Lauren Nicol Roberta Croce
author_sort	Lauren Nicol
title	The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII
title_short	The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII
title_full	The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII
title_fullStr	The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII
title_full_unstemmed	The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII
title_sort	psbs protein and low ph are necessary and sufficient to induce quenching in the light-harvesting complex of plants lhcii
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/85784a06f2774c49ba2955845b7494c0
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The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII

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