The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation

Abstract Sporulation in Dictyostelium fruiting bodies evolved from amoebozoan encystation with both being induced by cAMP acting on PKA, but with downstream components still being unknown. Using tagged mutagenesis to find missing pathway components, we identified a sporeless mutant defective in a nu...

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Autores principales:	Yoko Yamada, Andrew Cassidy, Pauline Schaap
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85790f3ed99d4d13a1eae699f40d23b4
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spelling	oai:doaj.org-article:85790f3ed99d4d13a1eae699f40d23b42021-12-02T15:07:59ZThe transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation10.1038/s41598-018-24915-w2045-2322https://doaj.org/article/85790f3ed99d4d13a1eae699f40d23b42018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24915-whttps://doaj.org/toc/2045-2322Abstract Sporulation in Dictyostelium fruiting bodies evolved from amoebozoan encystation with both being induced by cAMP acting on PKA, but with downstream components still being unknown. Using tagged mutagenesis to find missing pathway components, we identified a sporeless mutant defective in a nuclear protein, SpaA. Expression of prespore genes was strongly reduced in spaA- cells, while expression of many spore stage genes was absent. Chromatin immunoprecipitation (ChIP) of a SpaA-YFP gene fusion showed that (pre)spore gene promoters bind directly to SpaA, identifying SpaA as a transcriptional regulator. SpaA dependent spore gene expression required PKA in vivo and was stimulated in vitro by the membrane-permeant PKA agonist 8Br-cAMP. The PKA agonist also promoted SpaA binding to (pre)spore promoters, placing SpaA downstream of PKA. Sequencing of SpaA-YFP ChIPed DNA fragments revealed that SpaA binds at least 117 (pre)spore promoters, including those of other transcription factors that activate some spore genes. These factors are not in turn required for spaA expression, identifying SpaA as the major trancriptional inducer of sporulation.Yoko YamadaAndrew CassidyPauline SchaapNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoko Yamada Andrew Cassidy Pauline Schaap The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation
description	Abstract Sporulation in Dictyostelium fruiting bodies evolved from amoebozoan encystation with both being induced by cAMP acting on PKA, but with downstream components still being unknown. Using tagged mutagenesis to find missing pathway components, we identified a sporeless mutant defective in a nuclear protein, SpaA. Expression of prespore genes was strongly reduced in spaA- cells, while expression of many spore stage genes was absent. Chromatin immunoprecipitation (ChIP) of a SpaA-YFP gene fusion showed that (pre)spore gene promoters bind directly to SpaA, identifying SpaA as a transcriptional regulator. SpaA dependent spore gene expression required PKA in vivo and was stimulated in vitro by the membrane-permeant PKA agonist 8Br-cAMP. The PKA agonist also promoted SpaA binding to (pre)spore promoters, placing SpaA downstream of PKA. Sequencing of SpaA-YFP ChIPed DNA fragments revealed that SpaA binds at least 117 (pre)spore promoters, including those of other transcription factors that activate some spore genes. These factors are not in turn required for spaA expression, identifying SpaA as the major trancriptional inducer of sporulation.
format	article
author	Yoko Yamada Andrew Cassidy Pauline Schaap
author_facet	Yoko Yamada Andrew Cassidy Pauline Schaap
author_sort	Yoko Yamada
title	The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation
title_short	The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation
title_full	The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation
title_fullStr	The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation
title_full_unstemmed	The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation
title_sort	transcription factor spores absent a is a pka dependent inducer of dictyostelium sporulation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/85790f3ed99d4d13a1eae699f40d23b4
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The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation

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