Identification of novel avian influenza virus derived CD8+ T-cell epitopes.

Avian influenza virus (AIV) infection is a continuing threat to both humans and poultry. Influenza virus specific CD8+ T cells are associated with protection against homologous and heterologous influenza strains. In contrast to what has been described for humans and mice, knowledge on epitope-specif...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sylvia S N Reemers, Daphne A van Haarlem, Alice J A M Sijts, Lonneke Vervelde, Christine A Jansen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/857db7ed68ce4d98908e34a37b8cb26d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:857db7ed68ce4d98908e34a37b8cb26d
record_format	dspace
spelling	oai:doaj.org-article:857db7ed68ce4d98908e34a37b8cb26d2021-11-18T07:26:55ZIdentification of novel avian influenza virus derived CD8+ T-cell epitopes.1932-620310.1371/journal.pone.0031953https://doaj.org/article/857db7ed68ce4d98908e34a37b8cb26d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22384112/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Avian influenza virus (AIV) infection is a continuing threat to both humans and poultry. Influenza virus specific CD8+ T cells are associated with protection against homologous and heterologous influenza strains. In contrast to what has been described for humans and mice, knowledge on epitope-specific CD8+ T cells in chickens is limited. Therefore, we set out to identify AIV-specific CD8+ T-cell epitopes. Epitope predictions based on anchor residues resulted in 33 candidate epitopes. MHC I inbred chickens were infected with a low pathogenic AIV strain and sacrificed at 5, 7, 10 and 14 days post infection (dpi). Lymphocytes isolated from lung, spleen and blood were stimulated ex vivo with AIV-specific pooled or individual peptides and the production of IFNγ was determined by ELIspot. This resulted in the identification of 12 MHC B12-restricted, 3 B4-restricted and 1 B19-restricted AIV- specific CD8+ T-cell epitopes. In conclusion, we have identified novel AIV-derived CD8+ T-cell epitopes for several inbred chicken strains. This knowledge can be used to study the role of CD8+ T cells against AIV infection in a natural host for influenza, and may be important for vaccine development.Sylvia S N ReemersDaphne A van HaarlemAlice J A M SijtsLonneke VerveldeChristine A JansenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31953 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sylvia S N Reemers Daphne A van Haarlem Alice J A M Sijts Lonneke Vervelde Christine A Jansen Identification of novel avian influenza virus derived CD8+ T-cell epitopes.
description	Avian influenza virus (AIV) infection is a continuing threat to both humans and poultry. Influenza virus specific CD8+ T cells are associated with protection against homologous and heterologous influenza strains. In contrast to what has been described for humans and mice, knowledge on epitope-specific CD8+ T cells in chickens is limited. Therefore, we set out to identify AIV-specific CD8+ T-cell epitopes. Epitope predictions based on anchor residues resulted in 33 candidate epitopes. MHC I inbred chickens were infected with a low pathogenic AIV strain and sacrificed at 5, 7, 10 and 14 days post infection (dpi). Lymphocytes isolated from lung, spleen and blood were stimulated ex vivo with AIV-specific pooled or individual peptides and the production of IFNγ was determined by ELIspot. This resulted in the identification of 12 MHC B12-restricted, 3 B4-restricted and 1 B19-restricted AIV- specific CD8+ T-cell epitopes. In conclusion, we have identified novel AIV-derived CD8+ T-cell epitopes for several inbred chicken strains. This knowledge can be used to study the role of CD8+ T cells against AIV infection in a natural host for influenza, and may be important for vaccine development.
format	article
author	Sylvia S N Reemers Daphne A van Haarlem Alice J A M Sijts Lonneke Vervelde Christine A Jansen
author_facet	Sylvia S N Reemers Daphne A van Haarlem Alice J A M Sijts Lonneke Vervelde Christine A Jansen
author_sort	Sylvia S N Reemers
title	Identification of novel avian influenza virus derived CD8+ T-cell epitopes.
title_short	Identification of novel avian influenza virus derived CD8+ T-cell epitopes.
title_full	Identification of novel avian influenza virus derived CD8+ T-cell epitopes.
title_fullStr	Identification of novel avian influenza virus derived CD8+ T-cell epitopes.
title_full_unstemmed	Identification of novel avian influenza virus derived CD8+ T-cell epitopes.
title_sort	identification of novel avian influenza virus derived cd8+ t-cell epitopes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/857db7ed68ce4d98908e34a37b8cb26d
work_keys_str_mv	AT sylviasnreemers identificationofnovelavianinfluenzavirusderivedcd8tcellepitopes AT daphneavanhaarlem identificationofnovelavianinfluenzavirusderivedcd8tcellepitopes AT alicejamsijts identificationofnovelavianinfluenzavirusderivedcd8tcellepitopes AT lonnekevervelde identificationofnovelavianinfluenzavirusderivedcd8tcellepitopes AT christineajansen identificationofnovelavianinfluenzavirusderivedcd8tcellepitopes
_version_	1718423422392860672

Identification of novel avian influenza virus derived CD8+ T-cell epitopes.

Ejemplares similares