Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid

A combined vaccine of Equine influenza (EI), equine herpesvirus–1 (EHV-1), rabies virus and tetanus toxoid adjuvanted with saponin and Alhydrogel was prepared. Quality control testing of such vaccine revealed that it was free from foreign contaminants, safe for Guinea-pigs, mice and mares. It was in...

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Autores principales:	Safaa A. Warda, Eman M. Ebied, Nashwa K. Madkour, Fatma F.Warda, Omaima A.A. Al-Shamandy, Wafaa R. Abdelaziz, Hala El Sawy, Attyat M. Kotb
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	vaccine equine influenza herpesvirus-1 rabies tetanus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.140096 https://doaj.org/article/858867481ddc4fe596e154da14536b1f
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spelling	oai:doaj.org-article:858867481ddc4fe596e154da14536b1f2021-12-02T14:02:57ZTrials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoidhttps://dx.doi.org/10.21608/javs.2021.1400961687-40722090-3308https://doaj.org/article/858867481ddc4fe596e154da14536b1f2021-01-01T00:00:00Zhttps://javs.journals.ekb.eg/article_140096.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308A combined vaccine of Equine influenza (EI), equine herpesvirus–1 (EHV-1), rabies virus and tetanus toxoid adjuvanted with saponin and Alhydrogel was prepared. Quality control testing of such vaccine revealed that it was free from foreign contaminants, safe for Guinea-pigs, mice and mares. It was inoculated into Guinea -pigs as a preliminary evaluation for its potency and it was proven safe and potent. Also, it exceeded the permissible protective level allowed to be used for the vaccination of horses. Two groups of mares were used for evaluating the potency of the vaccine. The first one received two doses (5 ml) of the prepared vaccine with 4 weeks interval while the 2nd group was kept without vaccination as control. The mean haemagglutination inhibition (HI) influenza antibody titer reached its maximum at the 2nd month post-vaccination (MPV); 2048 while EHV-1 antibodies reached the peak at the 3rd MPV as recorded by ELISA and neutralizing indices; 1790 and 3.50. Rabies antibodies were detectable in vaccinated mares by the 2nd week post-vaccination showing titers of 16 and 1.0 by serum neutralization test (SNT) and ELISA, respectively recording their peaks (128 by SNT and 2.15 by ELISA) at the 2nd MPV. Tetanus antitoxic titer increased till reaching the peak at the 2nd MPV (40IU/ml) as determined by toxin neutralization test. Depending on these results, it could be concluded that the prepared inactivated EI, EHV-1, rabies and tetanus vaccine is safe and potent for mares providing them with good protective levels of specific antibodies against the 4 used antigens up to 6 months.Safaa A. WardaEman M. Ebied Nashwa K. MadkourFatma F.WardaOmaima A.A. Al-ShamandyWafaa R. AbdelazizHala El SawyAttyat M. KotbEgyptian Society for Animal Managementarticlevaccineequine influenzaherpesvirus-1rabiestetanusZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 1, Pp 38-44 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vaccine equine influenza herpesvirus-1 rabies tetanus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	vaccine equine influenza herpesvirus-1 rabies tetanus Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Safaa A. Warda Eman M. Ebied Nashwa K. Madkour Fatma F.Warda Omaima A.A. Al-Shamandy Wafaa R. Abdelaziz Hala El Sawy Attyat M. Kotb Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid
description	A combined vaccine of Equine influenza (EI), equine herpesvirus–1 (EHV-1), rabies virus and tetanus toxoid adjuvanted with saponin and Alhydrogel was prepared. Quality control testing of such vaccine revealed that it was free from foreign contaminants, safe for Guinea-pigs, mice and mares. It was inoculated into Guinea -pigs as a preliminary evaluation for its potency and it was proven safe and potent. Also, it exceeded the permissible protective level allowed to be used for the vaccination of horses. Two groups of mares were used for evaluating the potency of the vaccine. The first one received two doses (5 ml) of the prepared vaccine with 4 weeks interval while the 2nd group was kept without vaccination as control. The mean haemagglutination inhibition (HI) influenza antibody titer reached its maximum at the 2nd month post-vaccination (MPV); 2048 while EHV-1 antibodies reached the peak at the 3rd MPV as recorded by ELISA and neutralizing indices; 1790 and 3.50. Rabies antibodies were detectable in vaccinated mares by the 2nd week post-vaccination showing titers of 16 and 1.0 by serum neutralization test (SNT) and ELISA, respectively recording their peaks (128 by SNT and 2.15 by ELISA) at the 2nd MPV. Tetanus antitoxic titer increased till reaching the peak at the 2nd MPV (40IU/ml) as determined by toxin neutralization test. Depending on these results, it could be concluded that the prepared inactivated EI, EHV-1, rabies and tetanus vaccine is safe and potent for mares providing them with good protective levels of specific antibodies against the 4 used antigens up to 6 months.
format	article
author	Safaa A. Warda Eman M. Ebied Nashwa K. Madkour Fatma F.Warda Omaima A.A. Al-Shamandy Wafaa R. Abdelaziz Hala El Sawy Attyat M. Kotb
author_facet	Safaa A. Warda Eman M. Ebied Nashwa K. Madkour Fatma F.Warda Omaima A.A. Al-Shamandy Wafaa R. Abdelaziz Hala El Sawy Attyat M. Kotb
author_sort	Safaa A. Warda
title	Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid
title_short	Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid
title_full	Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid
title_fullStr	Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid
title_full_unstemmed	Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid
title_sort	trials for preparation of combined vaccine of equine influenza, equine herpesvirus–1, rabies virus and tetanus toxoid
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.140096 https://doaj.org/article/858867481ddc4fe596e154da14536b1f
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Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid

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