Live cell dynamics of the NF-Y transcription factor

Abstract Transcription factors (TFs) are core players in the control of gene expression, evolutionarily selected to recognise a subset of specific DNA sequences and nucleate the recruitment of the transcriptional machinery. How TFs assemble and move in the nucleus to locate and bind their DNA target...

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Autores principales:	David G. Priest, Andrea Bernardini, Jieqiong Lou, Roberto Mantovani, Elizabeth Hinde
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8593dee152d94a6781418e818c37be23
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spelling	oai:doaj.org-article:8593dee152d94a6781418e818c37be232021-12-02T14:47:37ZLive cell dynamics of the NF-Y transcription factor10.1038/s41598-021-90081-12045-2322https://doaj.org/article/8593dee152d94a6781418e818c37be232021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90081-1https://doaj.org/toc/2045-2322Abstract Transcription factors (TFs) are core players in the control of gene expression, evolutionarily selected to recognise a subset of specific DNA sequences and nucleate the recruitment of the transcriptional machinery. How TFs assemble and move in the nucleus to locate and bind their DNA targets and cause a transcriptional response, remains mostly unclear. NF-Y is a highly conserved, heterotrimeric TF with important roles in both housekeeping and lineage-specific gene expression, functioning as a promoter organiser. Despite a large number of biochemical, structural and genomic studies of NF-Y, there is a lack of experiments in single living cells; therefore, basic assumptions of NF-Y biology remain unproven in vivo. Here we employ a series of dynamic fluorescence microscopy methods (FLIM-FRET, NB, RICS and FRAP) to study NF-Y dynamics and complex formation in live cells. Specifically, we provide quantitative measurement of NF-Y subunit association and diffusion kinetics in the nucleus that collectively suggest NF-Y to move and bind chromatin as a trimeric complex in vivo.David G. PriestAndrea BernardiniJieqiong LouRoberto MantovaniElizabeth HindeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David G. Priest Andrea Bernardini Jieqiong Lou Roberto Mantovani Elizabeth Hinde Live cell dynamics of the NF-Y transcription factor
description	Abstract Transcription factors (TFs) are core players in the control of gene expression, evolutionarily selected to recognise a subset of specific DNA sequences and nucleate the recruitment of the transcriptional machinery. How TFs assemble and move in the nucleus to locate and bind their DNA targets and cause a transcriptional response, remains mostly unclear. NF-Y is a highly conserved, heterotrimeric TF with important roles in both housekeeping and lineage-specific gene expression, functioning as a promoter organiser. Despite a large number of biochemical, structural and genomic studies of NF-Y, there is a lack of experiments in single living cells; therefore, basic assumptions of NF-Y biology remain unproven in vivo. Here we employ a series of dynamic fluorescence microscopy methods (FLIM-FRET, NB, RICS and FRAP) to study NF-Y dynamics and complex formation in live cells. Specifically, we provide quantitative measurement of NF-Y subunit association and diffusion kinetics in the nucleus that collectively suggest NF-Y to move and bind chromatin as a trimeric complex in vivo.
format	article
author	David G. Priest Andrea Bernardini Jieqiong Lou Roberto Mantovani Elizabeth Hinde
author_facet	David G. Priest Andrea Bernardini Jieqiong Lou Roberto Mantovani Elizabeth Hinde
author_sort	David G. Priest
title	Live cell dynamics of the NF-Y transcription factor
title_short	Live cell dynamics of the NF-Y transcription factor
title_full	Live cell dynamics of the NF-Y transcription factor
title_fullStr	Live cell dynamics of the NF-Y transcription factor
title_full_unstemmed	Live cell dynamics of the NF-Y transcription factor
title_sort	live cell dynamics of the nf-y transcription factor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8593dee152d94a6781418e818c37be23
work_keys_str_mv	AT davidgpriest livecelldynamicsofthenfytranscriptionfactor AT andreabernardini livecelldynamicsofthenfytranscriptionfactor AT jieqionglou livecelldynamicsofthenfytranscriptionfactor AT robertomantovani livecelldynamicsofthenfytranscriptionfactor AT elizabethhinde livecelldynamicsofthenfytranscriptionfactor
_version_	1718389521332043776

Live cell dynamics of the NF-Y transcription factor

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