Expression profile of intestinal stem cell markers in colitis-associated carcinogenesis

Abstract The intestinal epithelium has two distinct two stem cell populations, namely, crypt base columnar (CBC) cells and +4 cells. Several specific markers have been identified for each stem cell population. In this study, we examined the expression profiles of these markers in colitis-associated...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hye Sung Kim, Cheol Lee, Woo Ho Kim, Young Hee Maeng, Bo Gun Jang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85953e3678274e40ae074975d1f1feec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:85953e3678274e40ae074975d1f1feec
record_format	dspace
spelling	oai:doaj.org-article:85953e3678274e40ae074975d1f1feec2021-12-02T16:06:58ZExpression profile of intestinal stem cell markers in colitis-associated carcinogenesis10.1038/s41598-017-06900-x2045-2322https://doaj.org/article/85953e3678274e40ae074975d1f1feec2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06900-xhttps://doaj.org/toc/2045-2322Abstract The intestinal epithelium has two distinct two stem cell populations, namely, crypt base columnar (CBC) cells and +4 cells. Several specific markers have been identified for each stem cell population. In this study, we examined the expression profiles of these markers in colitis-associated carcinogenesis (CAC) to investigate whether they can be used as biomarkers for the early detection of dysplasia. The expression of intestinal stem cell (ISC) markers was measured by real-time polymerase chain reaction during CAC that was induced by azoxymethane and dextran sodium sulfate treatment. CBC stem cell markers increased continuously with tumor development, whereas a +4 cell expression profile was not present. CBC stem cell population was suppressed in the acute colitis and then expanded to repopulate the crypts during the regeneration period. Notably, RNA in situ hybridization revealed that all dysplasia and cancer samples showed increased expression of CBC stem cell markers in more than one-third of the tumor height, whereas regenerative glands had CBC stem cell markers confined to the lower one-third of the crypt. These results suggest that CBC stem cell markers could be a useful tool for the early detection of colitis-induced tumors.Hye Sung KimCheol LeeWoo Ho KimYoung Hee MaengBo Gun JangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hye Sung Kim Cheol Lee Woo Ho Kim Young Hee Maeng Bo Gun Jang Expression profile of intestinal stem cell markers in colitis-associated carcinogenesis
description	Abstract The intestinal epithelium has two distinct two stem cell populations, namely, crypt base columnar (CBC) cells and +4 cells. Several specific markers have been identified for each stem cell population. In this study, we examined the expression profiles of these markers in colitis-associated carcinogenesis (CAC) to investigate whether they can be used as biomarkers for the early detection of dysplasia. The expression of intestinal stem cell (ISC) markers was measured by real-time polymerase chain reaction during CAC that was induced by azoxymethane and dextran sodium sulfate treatment. CBC stem cell markers increased continuously with tumor development, whereas a +4 cell expression profile was not present. CBC stem cell population was suppressed in the acute colitis and then expanded to repopulate the crypts during the regeneration period. Notably, RNA in situ hybridization revealed that all dysplasia and cancer samples showed increased expression of CBC stem cell markers in more than one-third of the tumor height, whereas regenerative glands had CBC stem cell markers confined to the lower one-third of the crypt. These results suggest that CBC stem cell markers could be a useful tool for the early detection of colitis-induced tumors.
format	article
author	Hye Sung Kim Cheol Lee Woo Ho Kim Young Hee Maeng Bo Gun Jang
author_facet	Hye Sung Kim Cheol Lee Woo Ho Kim Young Hee Maeng Bo Gun Jang
author_sort	Hye Sung Kim
title	Expression profile of intestinal stem cell markers in colitis-associated carcinogenesis
title_short	Expression profile of intestinal stem cell markers in colitis-associated carcinogenesis
title_full	Expression profile of intestinal stem cell markers in colitis-associated carcinogenesis
title_fullStr	Expression profile of intestinal stem cell markers in colitis-associated carcinogenesis
title_full_unstemmed	Expression profile of intestinal stem cell markers in colitis-associated carcinogenesis
title_sort	expression profile of intestinal stem cell markers in colitis-associated carcinogenesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/85953e3678274e40ae074975d1f1feec
work_keys_str_mv	AT hyesungkim expressionprofileofintestinalstemcellmarkersincolitisassociatedcarcinogenesis AT cheollee expressionprofileofintestinalstemcellmarkersincolitisassociatedcarcinogenesis AT woohokim expressionprofileofintestinalstemcellmarkersincolitisassociatedcarcinogenesis AT youngheemaeng expressionprofileofintestinalstemcellmarkersincolitisassociatedcarcinogenesis AT bogunjang expressionprofileofintestinalstemcellmarkersincolitisassociatedcarcinogenesis
_version_	1718384787606994944

Expression profile of intestinal stem cell markers in colitis-associated carcinogenesis

Ejemplares similares