Programming cell growth into different cluster shapes using diffusible signals.

Advances in genetic engineering technologies have allowed the construction of artificial genetic circuits, which have been used to generate spatial patterns of differential gene expression. However, the question of how cells can be programmed, and how complex the rules need to be, to achieve a desir...

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Autores principales:	Yipei Guo, Mor Nitzan, Michael P Brenner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/85a2c56e6abf4ee6bfbf13147ce55aa5
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id	oai:doaj.org-article:85a2c56e6abf4ee6bfbf13147ce55aa5
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spelling	oai:doaj.org-article:85a2c56e6abf4ee6bfbf13147ce55aa52021-12-02T19:57:58ZProgramming cell growth into different cluster shapes using diffusible signals.1553-734X1553-735810.1371/journal.pcbi.1009576https://doaj.org/article/85a2c56e6abf4ee6bfbf13147ce55aa52021-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pcbi.1009576https://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358Advances in genetic engineering technologies have allowed the construction of artificial genetic circuits, which have been used to generate spatial patterns of differential gene expression. However, the question of how cells can be programmed, and how complex the rules need to be, to achieve a desired tissue morphology has received less attention. Here, we address these questions by developing a mathematical model to study how cells can collectively grow into clusters with different structural morphologies by secreting diffusible signals that can influence cellular growth rates. We formulate how growth regulators can be used to control the formation of cellular protrusions and how the range of achievable structures scales with the number of distinct signals. We show that a single growth inhibitor is insufficient for the formation of multiple protrusions but may be achieved with multiple growth inhibitors, and that other types of signals can regulate the shape of protrusion tips. These examples illustrate how our approach could potentially be used to guide the design of regulatory circuits for achieving a desired target structure.Yipei GuoMor NitzanMichael P BrennerPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 17, Iss 11, p e1009576 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Yipei Guo Mor Nitzan Michael P Brenner Programming cell growth into different cluster shapes using diffusible signals.
description	Advances in genetic engineering technologies have allowed the construction of artificial genetic circuits, which have been used to generate spatial patterns of differential gene expression. However, the question of how cells can be programmed, and how complex the rules need to be, to achieve a desired tissue morphology has received less attention. Here, we address these questions by developing a mathematical model to study how cells can collectively grow into clusters with different structural morphologies by secreting diffusible signals that can influence cellular growth rates. We formulate how growth regulators can be used to control the formation of cellular protrusions and how the range of achievable structures scales with the number of distinct signals. We show that a single growth inhibitor is insufficient for the formation of multiple protrusions but may be achieved with multiple growth inhibitors, and that other types of signals can regulate the shape of protrusion tips. These examples illustrate how our approach could potentially be used to guide the design of regulatory circuits for achieving a desired target structure.
format	article
author	Yipei Guo Mor Nitzan Michael P Brenner
author_facet	Yipei Guo Mor Nitzan Michael P Brenner
author_sort	Yipei Guo
title	Programming cell growth into different cluster shapes using diffusible signals.
title_short	Programming cell growth into different cluster shapes using diffusible signals.
title_full	Programming cell growth into different cluster shapes using diffusible signals.
title_fullStr	Programming cell growth into different cluster shapes using diffusible signals.
title_full_unstemmed	Programming cell growth into different cluster shapes using diffusible signals.
title_sort	programming cell growth into different cluster shapes using diffusible signals.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/85a2c56e6abf4ee6bfbf13147ce55aa5
work_keys_str_mv	AT yipeiguo programmingcellgrowthintodifferentclustershapesusingdiffusiblesignals AT mornitzan programmingcellgrowthintodifferentclustershapesusingdiffusiblesignals AT michaelpbrenner programmingcellgrowthintodifferentclustershapesusingdiffusiblesignals
_version_	1718375788401655808

Programming cell growth into different cluster shapes using diffusible signals.

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