Effectiveness of Augmentative Biological Control of Streptomyces griseorubens UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against Neoscytalidium dimidiatum

To manage stem canker disease on royal poinciana, actinobacterial isolates were used as biological control agents (BCAs) based on their strong in vitro inhibitory effects against Neoscytalidiumdimidiatum. Streptomyces griseorubens<...

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Autores principales:	Bader M. Al Hamad, Seham M. Al Raish, Gaber A. Ramadan, Esam Eldin Saeed, Shaikha S. A. Alameri, Salima S. Al Senaani, Synan F. AbuQamar, Khaled A. El-Tarabily
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ACC deaminase actinobacteria biological control agent <i>Neoscytalidium dimidiatum</i> stem canker <i>Streptomyces</i> Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/85a6d9526c084e77aa0db8831d814b84
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spelling	oai:doaj.org-article:85a6d9526c084e77aa0db8831d814b842021-11-25T18:05:26ZEffectiveness of Augmentative Biological Control of <i>Streptomyces griseorubens</i> UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against <i>Neoscytalidium dimidiatum</i>10.3390/jof71108852309-608Xhttps://doaj.org/article/85a6d9526c084e77aa0db8831d814b842021-10-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/885https://doaj.org/toc/2309-608XTo manage stem canker disease on royal poinciana, actinobacterial isolates were used as biological control agents (BCAs) based on their strong <i>in vitro</i> inhibitory effects against <i>Neoscytalidium</i><i>dimidiatum</i>. <i>Streptomyces griseorubens</i> UAE2 and <i>Streptomyces wuyuanensis</i> UAE1 had the ability to produce antifungal compounds and cell-wall-degrading enzymes (CWDEs). Only <i>S. griseorubens</i>, however, restored the activity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD). <i>In vivo</i> apple fruit bioassay showed that lesion development was successfully constrained by either isolates on fruits inoculated with <i>N. dimidiatum</i>. In our greenhouse and container nursery experiments, <i>S. griseorubens</i> showed almost complete suppression of disease symptoms. This was evident when the preventive treatment of <i>S. griseorubens</i> significantly (<i>p</i> < 0.05) reduced the numbers of conidia of <i>N. dimidiatum</i> and defoliated leaves of royal poinciana seedlings to lesser levels than when <i>S. wuyuanensis</i> was applied, but comparable to control treatments (no pathogen). The disease management of stem canker was also associated with significant (<i>p</i> < 0.05) decreases in ACC levels in royal poinciana stems when <i>S. griseorubens</i> was applied compared to the non-ACCD-producing <i>S. wuyuanensis</i>. This study is the first to report the superiority of antagonistic actinobacteria to enhance their effectiveness as BCAs not only for producing antifungal metabolites and CWDEs but also for secreting ACCD.Bader M. Al HamadSeham M. Al RaishGaber A. RamadanEsam Eldin SaeedShaikha S. A. AlameriSalima S. Al SenaaniSynan F. AbuQamarKhaled A. El-TarabilyMDPI AGarticleACC deaminaseactinobacteriabiological control agent<i>Neoscytalidium dimidiatum</i>stem canker<i>Streptomyces</i>Biology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 885, p 885 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ACC deaminase actinobacteria biological control agent <i>Neoscytalidium dimidiatum</i> stem canker <i>Streptomyces</i> Biology (General) QH301-705.5
spellingShingle	ACC deaminase actinobacteria biological control agent <i>Neoscytalidium dimidiatum</i> stem canker <i>Streptomyces</i> Biology (General) QH301-705.5 Bader M. Al Hamad Seham M. Al Raish Gaber A. Ramadan Esam Eldin Saeed Shaikha S. A. Alameri Salima S. Al Senaani Synan F. AbuQamar Khaled A. El-Tarabily Effectiveness of Augmentative Biological Control of <i>Streptomyces griseorubens</i> UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against <i>Neoscytalidium dimidiatum</i>
description	To manage stem canker disease on royal poinciana, actinobacterial isolates were used as biological control agents (BCAs) based on their strong <i>in vitro</i> inhibitory effects against <i>Neoscytalidium</i><i>dimidiatum</i>. <i>Streptomyces griseorubens</i> UAE2 and <i>Streptomyces wuyuanensis</i> UAE1 had the ability to produce antifungal compounds and cell-wall-degrading enzymes (CWDEs). Only <i>S. griseorubens</i>, however, restored the activity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD). <i>In vivo</i> apple fruit bioassay showed that lesion development was successfully constrained by either isolates on fruits inoculated with <i>N. dimidiatum</i>. In our greenhouse and container nursery experiments, <i>S. griseorubens</i> showed almost complete suppression of disease symptoms. This was evident when the preventive treatment of <i>S. griseorubens</i> significantly (<i>p</i> < 0.05) reduced the numbers of conidia of <i>N. dimidiatum</i> and defoliated leaves of royal poinciana seedlings to lesser levels than when <i>S. wuyuanensis</i> was applied, but comparable to control treatments (no pathogen). The disease management of stem canker was also associated with significant (<i>p</i> < 0.05) decreases in ACC levels in royal poinciana stems when <i>S. griseorubens</i> was applied compared to the non-ACCD-producing <i>S. wuyuanensis</i>. This study is the first to report the superiority of antagonistic actinobacteria to enhance their effectiveness as BCAs not only for producing antifungal metabolites and CWDEs but also for secreting ACCD.
format	article
author	Bader M. Al Hamad Seham M. Al Raish Gaber A. Ramadan Esam Eldin Saeed Shaikha S. A. Alameri Salima S. Al Senaani Synan F. AbuQamar Khaled A. El-Tarabily
author_facet	Bader M. Al Hamad Seham M. Al Raish Gaber A. Ramadan Esam Eldin Saeed Shaikha S. A. Alameri Salima S. Al Senaani Synan F. AbuQamar Khaled A. El-Tarabily
author_sort	Bader M. Al Hamad
title	Effectiveness of Augmentative Biological Control of <i>Streptomyces griseorubens</i> UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against <i>Neoscytalidium dimidiatum</i>
title_short	Effectiveness of Augmentative Biological Control of <i>Streptomyces griseorubens</i> UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against <i>Neoscytalidium dimidiatum</i>
title_full	Effectiveness of Augmentative Biological Control of <i>Streptomyces griseorubens</i> UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against <i>Neoscytalidium dimidiatum</i>
title_fullStr	Effectiveness of Augmentative Biological Control of <i>Streptomyces griseorubens</i> UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against <i>Neoscytalidium dimidiatum</i>
title_full_unstemmed	Effectiveness of Augmentative Biological Control of <i>Streptomyces griseorubens</i> UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against <i>Neoscytalidium dimidiatum</i>
title_sort	effectiveness of augmentative biological control of <i>streptomyces griseorubens</i> uae2 depends on 1-aminocyclopropane-1-carboxylic acid deaminase activity against <i>neoscytalidium dimidiatum</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/85a6d9526c084e77aa0db8831d814b84
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Effectiveness of Augmentative Biological Control of <i>Streptomyces griseorubens</i> UAE2 Depends on 1-Aminocyclopropane-1-Carboxylic Acid Deaminase Activity against <i>Neoscytalidium dimidiatum</i>

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