Investigation of real-time photorepair activity on DNA via surface plasmon resonance.

The cyclobutane pyrimidine dimer (CPD) and 6-4 lesion formations along with the specific breaks on strands are the most common type of DNA damage caused by Ultraviolet light (UV) irradiation. CPD photolyase I and II construct two subfamilies of flavoproteins, which have recognition and repair capabi...

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Autores principales:	Rıza Kizilel, Enis Demir, Selimcan Azizoglu, Hande Asimgil, Ibrahim Halil Kavakli, Seda Kizilel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85a97e2125064f8eaca6bfb0d68196cf
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spelling	oai:doaj.org-article:85a97e2125064f8eaca6bfb0d68196cf2021-11-18T07:07:05ZInvestigation of real-time photorepair activity on DNA via surface plasmon resonance.1932-620310.1371/journal.pone.0044392https://doaj.org/article/85a97e2125064f8eaca6bfb0d68196cf2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952969/?tool=EBIhttps://doaj.org/toc/1932-6203The cyclobutane pyrimidine dimer (CPD) and 6-4 lesion formations along with the specific breaks on strands are the most common type of DNA damage caused by Ultraviolet light (UV) irradiation. CPD photolyase I and II construct two subfamilies of flavoproteins, which have recognition and repair capabilities of CPD sites on both single stranded (ssDNA) and double stranded DNA (dsDNA) with the aid of blue light energy. The other types of flavoprotein family consist of cryptochromes (CRY) that act as photoreceptors in plants, or circadian rhythm regulators in animals. Recent findings showed that a specific type of Cryptochrome-Drosophila, Arabidopsis, Synechocystis, Human (CRY-DASH) has photorepair activity on ssDNA. In this work, real-time interactions between CRY-DASH and ss/dsDNA as well as the interactions between Vibrio cholerae photolyase (VcPHR) and ss/dsDNA were investigated using Surface Plasmon Resonance (SPR). The interactions were then characterized and compared in order to investigate the effect of different types of flavoprotein on UV damaged ss/dsDNA. SPR results confirm the specific binding of VcPHR and CRY-DASH with UV treated DNA. This study is the first instance to quantify the interactions of UV treated and untreated DNA with flavoproteins.Rıza KizilelEnis DemirSelimcan AzizogluHande AsimgilIbrahim Halil KavakliSeda KizilelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e44392 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rıza Kizilel Enis Demir Selimcan Azizoglu Hande Asimgil Ibrahim Halil Kavakli Seda Kizilel Investigation of real-time photorepair activity on DNA via surface plasmon resonance.
description	The cyclobutane pyrimidine dimer (CPD) and 6-4 lesion formations along with the specific breaks on strands are the most common type of DNA damage caused by Ultraviolet light (UV) irradiation. CPD photolyase I and II construct two subfamilies of flavoproteins, which have recognition and repair capabilities of CPD sites on both single stranded (ssDNA) and double stranded DNA (dsDNA) with the aid of blue light energy. The other types of flavoprotein family consist of cryptochromes (CRY) that act as photoreceptors in plants, or circadian rhythm regulators in animals. Recent findings showed that a specific type of Cryptochrome-Drosophila, Arabidopsis, Synechocystis, Human (CRY-DASH) has photorepair activity on ssDNA. In this work, real-time interactions between CRY-DASH and ss/dsDNA as well as the interactions between Vibrio cholerae photolyase (VcPHR) and ss/dsDNA were investigated using Surface Plasmon Resonance (SPR). The interactions were then characterized and compared in order to investigate the effect of different types of flavoprotein on UV damaged ss/dsDNA. SPR results confirm the specific binding of VcPHR and CRY-DASH with UV treated DNA. This study is the first instance to quantify the interactions of UV treated and untreated DNA with flavoproteins.
format	article
author	Rıza Kizilel Enis Demir Selimcan Azizoglu Hande Asimgil Ibrahim Halil Kavakli Seda Kizilel
author_facet	Rıza Kizilel Enis Demir Selimcan Azizoglu Hande Asimgil Ibrahim Halil Kavakli Seda Kizilel
author_sort	Rıza Kizilel
title	Investigation of real-time photorepair activity on DNA via surface plasmon resonance.
title_short	Investigation of real-time photorepair activity on DNA via surface plasmon resonance.
title_full	Investigation of real-time photorepair activity on DNA via surface plasmon resonance.
title_fullStr	Investigation of real-time photorepair activity on DNA via surface plasmon resonance.
title_full_unstemmed	Investigation of real-time photorepair activity on DNA via surface plasmon resonance.
title_sort	investigation of real-time photorepair activity on dna via surface plasmon resonance.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/85a97e2125064f8eaca6bfb0d68196cf
work_keys_str_mv	AT rızakizilel investigationofrealtimephotorepairactivityondnaviasurfaceplasmonresonance AT enisdemir investigationofrealtimephotorepairactivityondnaviasurfaceplasmonresonance AT selimcanazizoglu investigationofrealtimephotorepairactivityondnaviasurfaceplasmonresonance AT handeasimgil investigationofrealtimephotorepairactivityondnaviasurfaceplasmonresonance AT ibrahimhalilkavakli investigationofrealtimephotorepairactivityondnaviasurfaceplasmonresonance AT sedakizilel investigationofrealtimephotorepairactivityondnaviasurfaceplasmonresonance
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Investigation of real-time photorepair activity on DNA via surface plasmon resonance.

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