Modulation of Production of Th1/Th2 Cytokines in Peripheral Blood Mononuclear Cells and Neutrophils by Hepatitis C Virus Infection in Chronically Infected Patients

This study investigated the influence of Hepatitis C virus (HCV) infection on the cytokine production profiles of the peripheral blood monoculear cells (PBMC) and neutrophils in chronically naïve HCV-infected patients. Seventy-five genotype-4 naïve HCV-infected patients (HCV+) and healthy subjects (...

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Autores principales:	Sahar Essa, Iqbal Siddique, Motaz Saad, Raj Raghupathy
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hepatitis C virus HCV genotypes neutrophils Th1 Th2 liver disease progression Medicine R
Acceso en línea:	https://doaj.org/article/85b0b7514abf469a9e0cbd422925f382
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spelling	oai:doaj.org-article:85b0b7514abf469a9e0cbd422925f3822021-11-25T18:38:54ZModulation of Production of Th1/Th2 Cytokines in Peripheral Blood Mononuclear Cells and Neutrophils by Hepatitis C Virus Infection in Chronically Infected Patients10.3390/pathogens101115192076-0817https://doaj.org/article/85b0b7514abf469a9e0cbd422925f3822021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1519https://doaj.org/toc/2076-0817This study investigated the influence of Hepatitis C virus (HCV) infection on the cytokine production profiles of the peripheral blood monoculear cells (PBMC) and neutrophils in chronically naïve HCV-infected patients. Seventy-five genotype-4 naïve HCV-infected patients (HCV+) and healthy subjects (HCV−) were enrolled. The neutrophils and the PBMC were separated by density gradient sedimentation and stimulated with a mitogen. The culture supernatants were evaluated for levels of IFN-α, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, and TNF-α using anti-cytokine antibody MACSPlex capture beads. The PBMC cytokine profiles of HCV+ patients showed significantly lower mean values for IFN-γ, IL-2, IL-6, IL-9, and IL-10 (<i>p</i> < 0.0001) as compared to HCV− subjects. In contrast, HCV+ patients showed higher mean levels of PBMC cytokine values for IL-5 and TNF-α (<i>p</i> < 0.0001). As for neutrophils, HCV+ patients showed significantly lower mean levels of IFN-α, IFN-γ, IL-2, IL-4, IL-6, IL-9, and IL-10 (<i>p</i> < 0.0001). In contrast, the neutrophils from HCV+ patients showed higher mean levels of IL-5, IL-12, and TNF-α (<i>p</i> < 0.0001). Th1–Th2 cytokine ratios suggested a lower Th1 bias in HCV+ subjects as compared to HCV− subjects. Our results suggest that chronic HCV infection brings about an immunomodulatory effect not only on neutrophils, but also to a lower extent on PBMCsSahar EssaIqbal SiddiqueMotaz SaadRaj RaghupathyMDPI AGarticlehepatitis C virusHCV genotypesneutrophilsTh1Th2liver disease progressionMedicineRENPathogens, Vol 10, Iss 1519, p 1519 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis C virus HCV genotypes neutrophils Th1 Th2 liver disease progression Medicine R
spellingShingle	hepatitis C virus HCV genotypes neutrophils Th1 Th2 liver disease progression Medicine R Sahar Essa Iqbal Siddique Motaz Saad Raj Raghupathy Modulation of Production of Th1/Th2 Cytokines in Peripheral Blood Mononuclear Cells and Neutrophils by Hepatitis C Virus Infection in Chronically Infected Patients
description	This study investigated the influence of Hepatitis C virus (HCV) infection on the cytokine production profiles of the peripheral blood monoculear cells (PBMC) and neutrophils in chronically naïve HCV-infected patients. Seventy-five genotype-4 naïve HCV-infected patients (HCV+) and healthy subjects (HCV−) were enrolled. The neutrophils and the PBMC were separated by density gradient sedimentation and stimulated with a mitogen. The culture supernatants were evaluated for levels of IFN-α, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, and TNF-α using anti-cytokine antibody MACSPlex capture beads. The PBMC cytokine profiles of HCV+ patients showed significantly lower mean values for IFN-γ, IL-2, IL-6, IL-9, and IL-10 (<i>p</i> < 0.0001) as compared to HCV− subjects. In contrast, HCV+ patients showed higher mean levels of PBMC cytokine values for IL-5 and TNF-α (<i>p</i> < 0.0001). As for neutrophils, HCV+ patients showed significantly lower mean levels of IFN-α, IFN-γ, IL-2, IL-4, IL-6, IL-9, and IL-10 (<i>p</i> < 0.0001). In contrast, the neutrophils from HCV+ patients showed higher mean levels of IL-5, IL-12, and TNF-α (<i>p</i> < 0.0001). Th1–Th2 cytokine ratios suggested a lower Th1 bias in HCV+ subjects as compared to HCV− subjects. Our results suggest that chronic HCV infection brings about an immunomodulatory effect not only on neutrophils, but also to a lower extent on PBMCs
format	article
author	Sahar Essa Iqbal Siddique Motaz Saad Raj Raghupathy
author_facet	Sahar Essa Iqbal Siddique Motaz Saad Raj Raghupathy
author_sort	Sahar Essa
title	Modulation of Production of Th1/Th2 Cytokines in Peripheral Blood Mononuclear Cells and Neutrophils by Hepatitis C Virus Infection in Chronically Infected Patients
title_short	Modulation of Production of Th1/Th2 Cytokines in Peripheral Blood Mononuclear Cells and Neutrophils by Hepatitis C Virus Infection in Chronically Infected Patients
title_full	Modulation of Production of Th1/Th2 Cytokines in Peripheral Blood Mononuclear Cells and Neutrophils by Hepatitis C Virus Infection in Chronically Infected Patients
title_fullStr	Modulation of Production of Th1/Th2 Cytokines in Peripheral Blood Mononuclear Cells and Neutrophils by Hepatitis C Virus Infection in Chronically Infected Patients
title_full_unstemmed	Modulation of Production of Th1/Th2 Cytokines in Peripheral Blood Mononuclear Cells and Neutrophils by Hepatitis C Virus Infection in Chronically Infected Patients
title_sort	modulation of production of th1/th2 cytokines in peripheral blood mononuclear cells and neutrophils by hepatitis c virus infection in chronically infected patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/85b0b7514abf469a9e0cbd422925f382
work_keys_str_mv	AT saharessa modulationofproductionofth1th2cytokinesinperipheralbloodmononuclearcellsandneutrophilsbyhepatitiscvirusinfectioninchronicallyinfectedpatients AT iqbalsiddique modulationofproductionofth1th2cytokinesinperipheralbloodmononuclearcellsandneutrophilsbyhepatitiscvirusinfectioninchronicallyinfectedpatients AT motazsaad modulationofproductionofth1th2cytokinesinperipheralbloodmononuclearcellsandneutrophilsbyhepatitiscvirusinfectioninchronicallyinfectedpatients AT rajraghupathy modulationofproductionofth1th2cytokinesinperipheralbloodmononuclearcellsandneutrophilsbyhepatitiscvirusinfectioninchronicallyinfectedpatients
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Modulation of Production of Th1/Th2 Cytokines in Peripheral Blood Mononuclear Cells and Neutrophils by Hepatitis C Virus Infection in Chronically Infected Patients

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