Species-Specific Recognition of <italic toggle="yes">Sulfolobales</italic> Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns

ABSTRACT The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-specific cellular aggregates. Within these aggregates, cells exchange DNA to repair DNA double-strand breaks via homologous recombination. Substitution of the Sulfolobus acidocaldarius pilin subunits UpsA a...

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Autores principales:	Marleen van Wolferen, Asif Shajahan, Kristina Heinrich, Susanne Brenzinger, Ian M. Black, Alexander Wagner, Ariane Briegel, Parastoo Azadi, Sonja-Verena Albers
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	type IV pili archaea Sulfolobus DNA exchange glycosylation species-specific recognition Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/85b42167652c4126972a5439f180f1d7
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spelling	oai:doaj.org-article:85b42167652c4126972a5439f180f1d72021-11-15T15:57:02ZSpecies-Specific Recognition of <italic toggle="yes">Sulfolobales</italic> Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns10.1128/mBio.03014-192150-7511https://doaj.org/article/85b42167652c4126972a5439f180f1d72020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.03014-19https://doaj.org/toc/2150-7511ABSTRACT The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-specific cellular aggregates. Within these aggregates, cells exchange DNA to repair DNA double-strand breaks via homologous recombination. Substitution of the Sulfolobus acidocaldarius pilin subunits UpsA and UpsB with their homologs from Sulfolobus tokodaii showed that these subunits facilitate species-specific aggregation. A region of low conservation within the UpsA homologs is primarily important for this specificity. Aggregation assays in the presence of different sugars showed the importance of N-glycosylation in the recognition process. In addition, the N-glycan decorating the S-layer of S. tokodaii is different from the one of S. acidocaldarius. Therefore, each Sulfolobus species seems to have developed a unique UpsA binding pocket and unique N-glycan composition to ensure aggregation and, consequently, also DNA exchange with cells from only the same species, which is essential for DNA repair by homologous recombination. IMPORTANCE Type IV pili can be found on the cell surface of many archaea and bacteria where they play important roles in different processes. The UV-inducible pili system of Sulfolobales (Ups) pili from the crenarchaeal Sulfolobales species are essential in establishing species-specific mating partners, thereby assisting in genome stability. With this work, we show that different Sulfolobus species have specific regions in their Ups pili subunits, which allow them to interact only with cells from the same species. Additionally, different Sulfolobus species have unique surface-layer N-glycosylation patterns. We propose that the unique features of each species allow the recognition of specific mating partners. This knowledge for the first time gives insights into the molecular basis of archaeal self-recognition.Marleen van WolferenAsif ShajahanKristina HeinrichSusanne BrenzingerIan M. BlackAlexander WagnerAriane BriegelParastoo AzadiSonja-Verena AlbersAmerican Society for Microbiologyarticletype IV piliarchaeaSulfolobusDNA exchangeglycosylationspecies-specific recognitionMicrobiologyQR1-502ENmBio, Vol 11, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	type IV pili archaea Sulfolobus DNA exchange glycosylation species-specific recognition Microbiology QR1-502
spellingShingle	type IV pili archaea Sulfolobus DNA exchange glycosylation species-specific recognition Microbiology QR1-502 Marleen van Wolferen Asif Shajahan Kristina Heinrich Susanne Brenzinger Ian M. Black Alexander Wagner Ariane Briegel Parastoo Azadi Sonja-Verena Albers Species-Specific Recognition of <italic toggle="yes">Sulfolobales</italic> Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns
description	ABSTRACT The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-specific cellular aggregates. Within these aggregates, cells exchange DNA to repair DNA double-strand breaks via homologous recombination. Substitution of the Sulfolobus acidocaldarius pilin subunits UpsA and UpsB with their homologs from Sulfolobus tokodaii showed that these subunits facilitate species-specific aggregation. A region of low conservation within the UpsA homologs is primarily important for this specificity. Aggregation assays in the presence of different sugars showed the importance of N-glycosylation in the recognition process. In addition, the N-glycan decorating the S-layer of S. tokodaii is different from the one of S. acidocaldarius. Therefore, each Sulfolobus species seems to have developed a unique UpsA binding pocket and unique N-glycan composition to ensure aggregation and, consequently, also DNA exchange with cells from only the same species, which is essential for DNA repair by homologous recombination. IMPORTANCE Type IV pili can be found on the cell surface of many archaea and bacteria where they play important roles in different processes. The UV-inducible pili system of Sulfolobales (Ups) pili from the crenarchaeal Sulfolobales species are essential in establishing species-specific mating partners, thereby assisting in genome stability. With this work, we show that different Sulfolobus species have specific regions in their Ups pili subunits, which allow them to interact only with cells from the same species. Additionally, different Sulfolobus species have unique surface-layer N-glycosylation patterns. We propose that the unique features of each species allow the recognition of specific mating partners. This knowledge for the first time gives insights into the molecular basis of archaeal self-recognition.
format	article
author	Marleen van Wolferen Asif Shajahan Kristina Heinrich Susanne Brenzinger Ian M. Black Alexander Wagner Ariane Briegel Parastoo Azadi Sonja-Verena Albers
author_facet	Marleen van Wolferen Asif Shajahan Kristina Heinrich Susanne Brenzinger Ian M. Black Alexander Wagner Ariane Briegel Parastoo Azadi Sonja-Verena Albers
author_sort	Marleen van Wolferen
title	Species-Specific Recognition of <italic toggle="yes">Sulfolobales</italic> Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns
title_short	Species-Specific Recognition of <italic toggle="yes">Sulfolobales</italic> Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns
title_full	Species-Specific Recognition of <italic toggle="yes">Sulfolobales</italic> Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns
title_fullStr	Species-Specific Recognition of <italic toggle="yes">Sulfolobales</italic> Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns
title_full_unstemmed	Species-Specific Recognition of <italic toggle="yes">Sulfolobales</italic> Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns
title_sort	species-specific recognition of <italic toggle="yes">sulfolobales</italic> mediated by uv-inducible pili and s-layer glycosylation patterns
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/85b42167652c4126972a5439f180f1d7
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Species-Specific Recognition of <italic toggle="yes">Sulfolobales</italic> Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns

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