Intracellular Trafficking and Persistence of <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Requires Transcription Factor EB

ABSTRACT Acinetobacter baumannii is a significant human pathogen associated with hospital-acquired infections. While adhesion, an initial and important step in A. baumannii infection, is well characterized, the intracellular trafficking of this pathogen inside host cells remains poorly studied. Here...

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Autores principales:	Raquel Parra-Millán, David Guerrero-Gómez, Rafael Ayerbe-Algaba, Maria Eugenia Pachón-Ibáñez, Antonio Miranda-Vizuete, Jerónimo Pachón, Younes Smani
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Acinetobacter baumannii Caenorhabditis elegans HLH-30 TFEB bacterial invasion Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/85b7e0b2cec04d61af9ca136ffb56114
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spelling	oai:doaj.org-article:85b7e0b2cec04d61af9ca136ffb561142021-11-15T15:22:14ZIntracellular Trafficking and Persistence of <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Requires Transcription Factor EB10.1128/mSphere.00106-182379-5042https://doaj.org/article/85b7e0b2cec04d61af9ca136ffb561142018-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00106-18https://doaj.org/toc/2379-5042ABSTRACT Acinetobacter baumannii is a significant human pathogen associated with hospital-acquired infections. While adhesion, an initial and important step in A. baumannii infection, is well characterized, the intracellular trafficking of this pathogen inside host cells remains poorly studied. Here, we demonstrate that transcription factor EB (TFEB) is activated after A. baumannii infection of human lung epithelial cells (A549). We also show that TFEB is required for the invasion and persistence inside A549 cells. Consequently, lysosomal biogenesis and autophagy activation were observed after TFEB activation which could increase the death of A549 cells. In addition, using the Caenorhabditis elegans infection model by A. baumannii, the TFEB orthologue HLH-30 was required for survival of the nematode to infection, although nuclear translocation of HLH-30 was not required. These results identify TFEB as a conserved key factor in the pathogenesis of A. baumannii. IMPORTANCE Adhesion is an initial and important step in Acinetobacter baumannii infections. However, the mechanism of entrance and persistence inside host cells is unclear and remains to be understood. In this study, we report that, in addition to its known role in host defense against Gram-positive bacterial infection, TFEB also plays an important role in the intracellular trafficking of A. baumannii in host cells. TFEB was activated shortly after A. baumannii infection and is required for its persistence within host cells. Additionally, using the C. elegans infection model by A. baumannii, the TFEB orthologue HLH-30 was required for survival of the nematode to infection, although nuclear translocation of HLH-30 was not required.Raquel Parra-MillánDavid Guerrero-GómezRafael Ayerbe-AlgabaMaria Eugenia Pachón-IbáñezAntonio Miranda-VizueteJerónimo PachónYounes SmaniAmerican Society for MicrobiologyarticleAcinetobacter baumanniiCaenorhabditis elegansHLH-30TFEBbacterial invasionMicrobiologyQR1-502ENmSphere, Vol 3, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acinetobacter baumannii Caenorhabditis elegans HLH-30 TFEB bacterial invasion Microbiology QR1-502
spellingShingle	Acinetobacter baumannii Caenorhabditis elegans HLH-30 TFEB bacterial invasion Microbiology QR1-502 Raquel Parra-Millán David Guerrero-Gómez Rafael Ayerbe-Algaba Maria Eugenia Pachón-Ibáñez Antonio Miranda-Vizuete Jerónimo Pachón Younes Smani Intracellular Trafficking and Persistence of <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Requires Transcription Factor EB
description	ABSTRACT Acinetobacter baumannii is a significant human pathogen associated with hospital-acquired infections. While adhesion, an initial and important step in A. baumannii infection, is well characterized, the intracellular trafficking of this pathogen inside host cells remains poorly studied. Here, we demonstrate that transcription factor EB (TFEB) is activated after A. baumannii infection of human lung epithelial cells (A549). We also show that TFEB is required for the invasion and persistence inside A549 cells. Consequently, lysosomal biogenesis and autophagy activation were observed after TFEB activation which could increase the death of A549 cells. In addition, using the Caenorhabditis elegans infection model by A. baumannii, the TFEB orthologue HLH-30 was required for survival of the nematode to infection, although nuclear translocation of HLH-30 was not required. These results identify TFEB as a conserved key factor in the pathogenesis of A. baumannii. IMPORTANCE Adhesion is an initial and important step in Acinetobacter baumannii infections. However, the mechanism of entrance and persistence inside host cells is unclear and remains to be understood. In this study, we report that, in addition to its known role in host defense against Gram-positive bacterial infection, TFEB also plays an important role in the intracellular trafficking of A. baumannii in host cells. TFEB was activated shortly after A. baumannii infection and is required for its persistence within host cells. Additionally, using the C. elegans infection model by A. baumannii, the TFEB orthologue HLH-30 was required for survival of the nematode to infection, although nuclear translocation of HLH-30 was not required.
format	article
author	Raquel Parra-Millán David Guerrero-Gómez Rafael Ayerbe-Algaba Maria Eugenia Pachón-Ibáñez Antonio Miranda-Vizuete Jerónimo Pachón Younes Smani
author_facet	Raquel Parra-Millán David Guerrero-Gómez Rafael Ayerbe-Algaba Maria Eugenia Pachón-Ibáñez Antonio Miranda-Vizuete Jerónimo Pachón Younes Smani
author_sort	Raquel Parra-Millán
title	Intracellular Trafficking and Persistence of <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Requires Transcription Factor EB
title_short	Intracellular Trafficking and Persistence of <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Requires Transcription Factor EB
title_full	Intracellular Trafficking and Persistence of <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Requires Transcription Factor EB
title_fullStr	Intracellular Trafficking and Persistence of <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Requires Transcription Factor EB
title_full_unstemmed	Intracellular Trafficking and Persistence of <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Requires Transcription Factor EB
title_sort	intracellular trafficking and persistence of <named-content content-type="genus-species">acinetobacter baumannii</named-content> requires transcription factor eb
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/85b7e0b2cec04d61af9ca136ffb56114
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Intracellular Trafficking and Persistence of <named-content content-type="genus-species">Acinetobacter baumannii</named-content> Requires Transcription Factor EB

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