Genome-wide association study identified candidate genes for seed size and seed composition improvement in M. truncatula

Abstract Grain legumes are highly valuable plant species, as they produce seeds with high protein content. Increasing seed protein production and improving seed nutritional quality represent an agronomical challenge in order to promote plant protein consumption of a growing population. In this study...

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Autores principales:	Zhijuan Chen, Vanessa Lancon-Verdier, Christine Le Signor, Yi-Min She, Yun Kang, Jerome Verdier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85bbd0906f0c4f42a5157291885db19d
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spelling	oai:doaj.org-article:85bbd0906f0c4f42a5157291885db19d2021-12-02T10:54:30ZGenome-wide association study identified candidate genes for seed size and seed composition improvement in M. truncatula10.1038/s41598-021-83581-72045-2322https://doaj.org/article/85bbd0906f0c4f42a5157291885db19d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83581-7https://doaj.org/toc/2045-2322Abstract Grain legumes are highly valuable plant species, as they produce seeds with high protein content. Increasing seed protein production and improving seed nutritional quality represent an agronomical challenge in order to promote plant protein consumption of a growing population. In this study, we used the genetic diversity, naturally present in Medicago truncatula, a model plant for legumes, to identify genes/loci regulating seed traits. Indeed, using sequencing data of 162 accessions from the Medicago HAPMAP collection, we performed genome-wide association study for 32 seed traits related to seed size and seed composition such as seed protein content/concentration, sulfur content/concentration. Using different GWAS and postGWAS methods, we identified 79 quantitative trait nucleotides (QTNs) as regulating seed size, 41 QTNs for seed composition related to nitrogen (i.e. storage protein) and sulfur (i.e. sulfur-containing amino acid) concentrations/contents. Furthermore, a strong positive correlation between seed size and protein content was revealed within the selected Medicago HAPMAP collection. In addition, several QTNs showed highly significant associations in different seed phenotypes for further functional validation studies, including one near an RNA-Binding Domain protein, which represents a valuable candidate as central regulator determining both seed size and composition. Finally, our findings in M. truncatula represent valuable resources to be exploitable in many legume crop species such as pea, common bean, and soybean due to its high synteny, which enable rapid transfer of these results into breeding programs and eventually help the improvement of legume grain production.Zhijuan ChenVanessa Lancon-VerdierChristine Le SignorYi-Min SheYun KangJerome VerdierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhijuan Chen Vanessa Lancon-Verdier Christine Le Signor Yi-Min She Yun Kang Jerome Verdier Genome-wide association study identified candidate genes for seed size and seed composition improvement in M. truncatula
description	Abstract Grain legumes are highly valuable plant species, as they produce seeds with high protein content. Increasing seed protein production and improving seed nutritional quality represent an agronomical challenge in order to promote plant protein consumption of a growing population. In this study, we used the genetic diversity, naturally present in Medicago truncatula, a model plant for legumes, to identify genes/loci regulating seed traits. Indeed, using sequencing data of 162 accessions from the Medicago HAPMAP collection, we performed genome-wide association study for 32 seed traits related to seed size and seed composition such as seed protein content/concentration, sulfur content/concentration. Using different GWAS and postGWAS methods, we identified 79 quantitative trait nucleotides (QTNs) as regulating seed size, 41 QTNs for seed composition related to nitrogen (i.e. storage protein) and sulfur (i.e. sulfur-containing amino acid) concentrations/contents. Furthermore, a strong positive correlation between seed size and protein content was revealed within the selected Medicago HAPMAP collection. In addition, several QTNs showed highly significant associations in different seed phenotypes for further functional validation studies, including one near an RNA-Binding Domain protein, which represents a valuable candidate as central regulator determining both seed size and composition. Finally, our findings in M. truncatula represent valuable resources to be exploitable in many legume crop species such as pea, common bean, and soybean due to its high synteny, which enable rapid transfer of these results into breeding programs and eventually help the improvement of legume grain production.
format	article
author	Zhijuan Chen Vanessa Lancon-Verdier Christine Le Signor Yi-Min She Yun Kang Jerome Verdier
author_facet	Zhijuan Chen Vanessa Lancon-Verdier Christine Le Signor Yi-Min She Yun Kang Jerome Verdier
author_sort	Zhijuan Chen
title	Genome-wide association study identified candidate genes for seed size and seed composition improvement in M. truncatula
title_short	Genome-wide association study identified candidate genes for seed size and seed composition improvement in M. truncatula
title_full	Genome-wide association study identified candidate genes for seed size and seed composition improvement in M. truncatula
title_fullStr	Genome-wide association study identified candidate genes for seed size and seed composition improvement in M. truncatula
title_full_unstemmed	Genome-wide association study identified candidate genes for seed size and seed composition improvement in M. truncatula
title_sort	genome-wide association study identified candidate genes for seed size and seed composition improvement in m. truncatula
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/85bbd0906f0c4f42a5157291885db19d
work_keys_str_mv	AT zhijuanchen genomewideassociationstudyidentifiedcandidategenesforseedsizeandseedcompositionimprovementinmtruncatula AT vanessalanconverdier genomewideassociationstudyidentifiedcandidategenesforseedsizeandseedcompositionimprovementinmtruncatula AT christinelesignor genomewideassociationstudyidentifiedcandidategenesforseedsizeandseedcompositionimprovementinmtruncatula AT yiminshe genomewideassociationstudyidentifiedcandidategenesforseedsizeandseedcompositionimprovementinmtruncatula AT yunkang genomewideassociationstudyidentifiedcandidategenesforseedsizeandseedcompositionimprovementinmtruncatula AT jeromeverdier genomewideassociationstudyidentifiedcandidategenesforseedsizeandseedcompositionimprovementinmtruncatula
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Genome-wide association study identified candidate genes for seed size and seed composition improvement in M. truncatula

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