Effect of the gene doublesex of anastrepha on the somatic sexual development of Drosophila.

<h4>Background</h4>The gene doublesex (dsx) is at the bottom of the sex determination genetic cascade and is transcribed in both sexes, but gives rise to two different proteins, DsxF and DsxM, which impose female and male sexual development respectively via the sex-specific regulation of...

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Autores principales:	Mercedes Alvarez, María Fernanda Ruiz, Lucas Sánchez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85bbfc94e3f24731bed32dc7ef579f2e
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spelling	oai:doaj.org-article:85bbfc94e3f24731bed32dc7ef579f2e2021-11-25T06:16:20ZEffect of the gene doublesex of anastrepha on the somatic sexual development of Drosophila.1932-620310.1371/journal.pone.0005141https://doaj.org/article/85bbfc94e3f24731bed32dc7ef579f2e2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19340310/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The gene doublesex (dsx) is at the bottom of the sex determination genetic cascade and is transcribed in both sexes, but gives rise to two different proteins, DsxF and DsxM, which impose female and male sexual development respectively via the sex-specific regulation of the so-called sexual cyto-differentiation genes. The present manuscript addressed the question about the functional conservation of the tephritid Anastrepha DsxF and DsxM proteins to direct the sexual development in Drosophila (Drosophilidae).<h4>Methodology</h4>To express these proteins in Drosophila, the GAL4-UAS system was used. The effect of these proteins was monitored in the sexually dimorphic regions of the fly: the foreleg basitarsus, the 5th, 6th and 7th tergites, and the external terminalia. In addition, we analysed the effect of Anastrepha DsxF and DsxM proteins on the regulation of Drosophila yolk protein genes, which are expressed in the fat body of adult females under the control of dsx.<h4>Conclusions</h4>The Anastrepha DsxF and DsxM proteins transformed doublesex intersexual Drosophila flies into females and males respectively, though this transformation was incomplete and the extent of their influence varied in the different sexually dimorphic regions of the adult fly. The Anastrepha DsxF and DsxM proteins also behaved as activators and repressors, respectively, of the Drosophila yolk protein genes, as do the DsxF and DsxM proteins of Drosophila itself. Finally, the Anastrepha DsxF and DsxM proteins were found to counteract the functions of Drosophila DsxM and DsxF respectively, reflecting the normal behaviour of the latter proteins towards one another. Collectively, these results indicate that the Anastrepha DsxF and DsxM proteins show conserved female and male sex-determination function respectively in Drosophila, though it appears that they cannot fully substitute the latter's own Dsx proteins. This incomplete function might be partly due to a reduced capacity of the Anastrepha Dsx proteins to completely control the Drosophila sexual cyto-differentiation genes, a consequence of the accumulation of divergence between these species resulting in the formation of different co-adapted complexes between the Dsx proteins and their target genes.Mercedes AlvarezMaría Fernanda RuizLucas SánchezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 4, p e5141 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mercedes Alvarez María Fernanda Ruiz Lucas Sánchez Effect of the gene doublesex of anastrepha on the somatic sexual development of Drosophila.
description	<h4>Background</h4>The gene doublesex (dsx) is at the bottom of the sex determination genetic cascade and is transcribed in both sexes, but gives rise to two different proteins, DsxF and DsxM, which impose female and male sexual development respectively via the sex-specific regulation of the so-called sexual cyto-differentiation genes. The present manuscript addressed the question about the functional conservation of the tephritid Anastrepha DsxF and DsxM proteins to direct the sexual development in Drosophila (Drosophilidae).<h4>Methodology</h4>To express these proteins in Drosophila, the GAL4-UAS system was used. The effect of these proteins was monitored in the sexually dimorphic regions of the fly: the foreleg basitarsus, the 5th, 6th and 7th tergites, and the external terminalia. In addition, we analysed the effect of Anastrepha DsxF and DsxM proteins on the regulation of Drosophila yolk protein genes, which are expressed in the fat body of adult females under the control of dsx.<h4>Conclusions</h4>The Anastrepha DsxF and DsxM proteins transformed doublesex intersexual Drosophila flies into females and males respectively, though this transformation was incomplete and the extent of their influence varied in the different sexually dimorphic regions of the adult fly. The Anastrepha DsxF and DsxM proteins also behaved as activators and repressors, respectively, of the Drosophila yolk protein genes, as do the DsxF and DsxM proteins of Drosophila itself. Finally, the Anastrepha DsxF and DsxM proteins were found to counteract the functions of Drosophila DsxM and DsxF respectively, reflecting the normal behaviour of the latter proteins towards one another. Collectively, these results indicate that the Anastrepha DsxF and DsxM proteins show conserved female and male sex-determination function respectively in Drosophila, though it appears that they cannot fully substitute the latter's own Dsx proteins. This incomplete function might be partly due to a reduced capacity of the Anastrepha Dsx proteins to completely control the Drosophila sexual cyto-differentiation genes, a consequence of the accumulation of divergence between these species resulting in the formation of different co-adapted complexes between the Dsx proteins and their target genes.
format	article
author	Mercedes Alvarez María Fernanda Ruiz Lucas Sánchez
author_facet	Mercedes Alvarez María Fernanda Ruiz Lucas Sánchez
author_sort	Mercedes Alvarez
title	Effect of the gene doublesex of anastrepha on the somatic sexual development of Drosophila.
title_short	Effect of the gene doublesex of anastrepha on the somatic sexual development of Drosophila.
title_full	Effect of the gene doublesex of anastrepha on the somatic sexual development of Drosophila.
title_fullStr	Effect of the gene doublesex of anastrepha on the somatic sexual development of Drosophila.
title_full_unstemmed	Effect of the gene doublesex of anastrepha on the somatic sexual development of Drosophila.
title_sort	effect of the gene doublesex of anastrepha on the somatic sexual development of drosophila.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/85bbfc94e3f24731bed32dc7ef579f2e
work_keys_str_mv	AT mercedesalvarez effectofthegenedoublesexofanastrephaonthesomaticsexualdevelopmentofdrosophila AT mariafernandaruiz effectofthegenedoublesexofanastrephaonthesomaticsexualdevelopmentofdrosophila AT lucassanchez effectofthegenedoublesexofanastrephaonthesomaticsexualdevelopmentofdrosophila
_version_	1718413994323083264

Effect of the gene doublesex of anastrepha on the somatic sexual development of Drosophila.

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